
TY  - JOUR
A1  - Byrne, A. T. 
A1  - Southgate, J. 
A1  - Brison, D. R. 
A1  - Leese, H. J. 
T1  - Effects of insulin-like growth factors I and II on tumour-necrosis-factor-<font face="symbol">a</font>-induced apoptosis in early murine embryos
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 2002
VL  - 14
IS  - 2
SP  - 79
EP  - 83
KW  - blastocyst, IGF, TNF&#945;.
UR  - https://doi.org/10.1071/RD01015 
N2  - The proposition that members of the insulin-like growth factor superfamily act as rescue factors from apoptosis in murine preimplantation embryos was tested. The cytokine tumour necrosis factor &#945; (TNF&#945;) was used to induce apoptosis. Zygotes were cultured for 5 days to the blastocyst stage in the presence or absence of TNF&#945; and in the presence or absence of the insulin-like growth factors, IGF-I or IGF-II. Tumour necrosis factor &#945; significantly increased the percentage of apoptotic cells and reduced the total cell count in Day 5 blastocysts. When IGF-I or IGF-II were added to the culture medium in the presence of TNF&#945;, the cell number and apoptotic dead cell index (DCI) were restored to control values. Insulin-like growth factor-I alone had a greater effect on total cell number than IGF-II alone, but did not significantly decrease the apoptotic DCI. In contrast, IGF-II significantly reduced the number of apoptotic cells. This study shows that IGFs may play a role as apoptotic survival factors in the early mouse embryo. 
ER  -

