
%0 Journal Article
%A Raghu, H. M. 
%A Nandi, S. 
%A Reddy, S. M. 
%T Follicle size and oocyte diameter in relation to developmental competence of buffalo oocytes <emph type="2">in vitro</emph>
%J Reproduction, Fertility and Development
%D 2002/02/21
%V 14
%N 1
%P 55-61
%K IVC, IVF, IVM.
%R https://doi.org/10.1071/RD01060
%U https://www.publish.csiro.au/paper/RD01060 
%X Follicular size, oocyte morphology and diameter were investigated for their possible relationship with in vitro developmental competence of buffalo oocytes. Cumulus oocytes complexes (COCs), aspirated from small (&#60;3 mm), medium (3&#8211;8 mm) and large (&#62;8 mm) follicles of normal ovaries and cystic ovarian follicles of abattoir-derived ovaries, were graded for their morphological appearance and were cultured to assess their developmental competence. The influence of cystic follicles on maturational competence of COCs recovered from co-existing follicles of cystic ovaries was studied. The mean diameter of oocytes from follicles of different size were examined, and the influence of oocyte diameter&#8212;(i) &#60;126 m; (ii) 127&#8211;144 m; (ii) 145&#8211;162 m; and (iv) &#62;163 m&#8212;on in vitro maturation, cleavage and embryo yield was studied. Results suggested that increased fertilization, cleavage and embryo development were significantly (P&#60;0.05) higher in COCs aspirated from large follicles, followed by medium and small-sized normal follicles, and the presence of cystic follicles had no significant (P&#60;0.05) effect on the maturation competence of the COCs recovered from co-existing follicles. The mean diameter of the buffalo oocyte obtained from normal ovaries was found to be 146.4 m and the rate of blastocyst production in vitro was significantly higher (P&#60;0.05) in oocytes with diameters greater than 145 m. In conclusion, the larger the size of the follicles and oocytes, the greater the developmental competence in vitro of buffalo oocytes. 
