
TY  - JOUR
A1  - Simasko, Steven M. 
A1  - Braileanu, Gheorghe T. 
A1  - Speth, Robert C. 
A1  - Mirando, Mark A. 
A1  - Daubert, Daisy 
A1  - Hu, Jianbo 
T1  - Angiotensin II increases intracellular calcium concentration in pig endometrial stromal cells through type 1 angiotensin receptors, but does not stimulate phospholipase C activity or prostaglandin F<emph type="8">2&#945;</emph> secretion
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 2002
VL  - 14
IS  - 4
SP  - 199
EP  - 205
KW  - endometrium, oxytocin.
UR  - https://doi.org/10.1071/RD02003 
N2  - Although the presence of endometrial receptors for angiotensin (Ang) II has been demonstrated, a specific function for AngII in the uterus has not been identified. Cytosolic free Ca2+ concentration [Ca2+]i, phospholipase C (PLC) activity and prostaglandin (PG) F2&#945; secretion in response to AngII and oxytocin (OT) were measured in pig endometrial stromal cells collected 16 days after oestrus. Treatment with 100 nm OT or AngII increased (P&#60;0.001) [Ca2+]i in stromal cells similarly (720 v. 690 m, respectively). Subsequent administration of OT or AngII to the same cells induced smaller [Ca2+]i increases (25% or 35% of the initial responses, respectively) that occurred only if the second exposure to the same agent took place at least 5 min after the first. When administered sequentially, OT and AngII each induced a full response within 1 min of the previous treatment, regardless of which peptide was applied first. Whereas OT increased PLC activity and PGF2&#945; secretion in stromal cells (P&#60;0.01), AngII did not increase either PLC activity or PGF2&#945; secretion. Type 1 AngII (AT1) receptors were present on stromal cells, whereas AT2 receptors were absent. Therefore, the effect of AngII in stromal cells was mediated via AT1 receptors. That AngII increased [Ca2+]i in stromal cells, but did not increase PLC or PGF2&#945; secretion, indicates that either AngII releases a pool of Ca2+ through a mechanism that is not mediated by PLC and is not involved in PGF2&#945; secretion or that a mechanism for PGF2&#945; production other than one involving Ca2+ may exist. 
ER  -

