
TY  - JOUR
A1  - Cleary, M. 
A1  - West, M. 
A1  - Shaw, J. 
A1  - Jenkin, G. 
A1  - Trounson, A. 
T1  - <emph type="2">In vitro</emph> maturation of oocytes from non-stimulated common wombats
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 2003
VL  - 15
IS  - 5
SP  - 303
EP  - 310
KW  - 
UR  - https://doi.org/10.1071/RD03042 
N2  - Assisted reproductive techniques, such as in vitro oocyte maturation in conjunction with in vitro fertilisation, may be used as a tool to manipulate reproduction. Using the common wombat as a model for the critically endangered northern hairy-nosed wombat, the present study examined whether oocyte maturation could be achieved under field conditions. At the time of collection, no oocytes were at the metaphase II (MII) stage (0/42). After 60 h culture using the submarine incubation system, 34% of oocytes (24/70) matured to telophase/MII, as indicated by the presence of a polar body. The proportion of oocytes that reached MII was higher for oocytes collected from follicles &#62;2 mm in diameter compared with follicles &#60;2 mm (40% v. 22%, respectively). The presence of cumulus cells alone did not influence the maturation potential. Oocytes without cumulus cells collected from follicles &#62;2 mm in diameter had the highest maturation rate (58%). Maturation was not affected by the reproductive status of the common wombat or a delay of up to 5 h before oocyte collection. In conclusion, the present study demonstrated that oocytes collected from non-stimulated common wombats can mature to MII in culture. 
ER  -

