
%0 Journal Article
%A McCoard, S. A. 
%A McNabb, W. C. 
%A Peterson, S. W. 
%A McCutcheon, S. N. 
%A Harris, P. M. 
%T Muscle growth, cell number, type and morphometry in single and twin fetal lambs during mid to late gestation
%J Reproduction, Fertility and Development
%D 2001/04/27
%V 12
%N 6
%P 319-327
%K development, myofibre hypertrophy, ontogeny, sheep.
%R https://doi.org/10.1071/RD99059
%U https://www.publish.csiro.au/paper/RD99059 
%X Muscle growth, myofibre number, type and morphometry were studied in large hindlimb muscles of single and twin fetal lambs during mid to late gestation. Placental insufficiency, evident by lower total placentome weight and number per fetus, resulted in reduced fetal weights from 100 to 140 days gestation in twins compared with singletons (at 140 days: 5016 108 g v. 5750 246 g, respectively; P&#60;0.05). However, competition between littermates did not consistently reduce muscle mass (15&#8211;22%) until 140 days gestation. Apparent myofibre number increased with age, indicating that the full complement of myofibres in some large hindlimb muscles may be achieved during early postnatal life. Litter size did not impact on apparent myofibre number in the semitendinosus, plantaris or gastrocnemius muscles. However, a transient effect on myofibre number in the adductor femoris muscle was observed from 80&#8211;120 days gestation. The phenotypic maturation of myofibres was unaffected by increasing litter size. Smaller muscle mass in twins was associated with smaller myofibre cross-sectional area in the semitendinosus, adductor femoris and gastrocnemius muscles at 140 days gestation. A similar trend was observed for the plantaris muscle. These results indicate that while competition between littermates for nutrients in late gestation can impact on both fetal and muscle mass, the fetus has the capacity to buffer against the effects of restricted nutrient supply on myofibre hyperplasia and phenotypic maturation, but myofibre hypertrophy is compromised. 
