
TY  - JOUR
A1  - Wales, RG 
A1  - Du, ZF 
T1  - Contribution of the pentose phosphate pathway to glucose utilization by preimplantation sheep embryos
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1993
VL  - 5
IS  - 3
SP  - 329
EP  - 340
KW  - 
UR  - https://doi.org/10.1071/RD9930329 
N2  - The activity of the pentose phosphate pathway of glucose metabolism in early sheep embryos and in the structures of the advanced conceptus from Day 13 to Day 19 of pregnancy was measured quantitatively during a 2.5-h incubation with glucose as sole energy source. For embryos during cleavage, activity of this pathway accounted for 6-9% of total glucose utilized. The proportion of glucose metabolized through the pentose pathway fell progressively with development and by Day 19 represented 1-2% of glucose turnover. However, total turnover of glucose increased eight fold between the 2-cell and blastocyst stage and the amount of glucose processed through the pentose pathway increased over this time despite the fall in the proportion utilized in this way. In contrast, glucose turnover by the advanced embryo and its extra embryonic membranes progressively decreased as the structures developed. As a result, estimates of the amount of glucose utilized through the pathway per microgram dried weight per hour declined to low values at Day 19 following the peak in activity at about the time of blastulation. Trophoblast and yolk sac processed less glucose through the pentose pathway per microgram dried weight than embryonic tissue but the allantois was similar to the embryo. Overall, the pentose pathway accounted for a relatively constant proportion of the CO2 produced from glucose under these experimental conditions with values generally between 15 and 20% of total CO2 produced. When activities in the components of the advanced conceptus were expressed as the total amount of glucose processed through the pathway per hour, turnover in the embryo, allantois and yolk sac increased progressively with time. By contrast, there was a substantial trough in the activity of the trophoblast on Day 17 of pregnancy. 
ER  -

