
TY  - JOUR
A1  - Iannello, RC 
A1  - Young, JC 
A1  - Sumarsono, S 
A1  - Tymms, MJ 
A1  - Kola, I 
T1  - Mouse testis Pdha-2 promoter upstream sequences confer tissue-and temporal-specific activity in transgenic mice
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1994
VL  - 6
IS  - 5
SP  - 599
EP  - 604
KW  - 
UR  - https://doi.org/10.1071/RD9940599 
N2  - Spermatogenesis is a complex process requiring the coordinate expression of a number of testis-specific genes. One of these, Pdha-2, codes for the murine testis-specific isoform of the E1 alpha subunit of the pyruvate dehydrogenase complex. To elucidate the mechanisms regulating its expression in vivo, we have begun to investigate the Pdha-2 promoter in transgenic mice. In this paper, a construct containing 3.0 kb of promoter and upstream sequences is reported to be sufficient for directing the testis-specific expression of a CAT reporter gene in mice harbouring the transgene. Similarly to the endogenous Pdha-2, the CAT gene is expressed in testis in a stage-specific manner. However, the 3.0-kb Pdha-2 promoter is not active in somatic tissue suggesting that repressor elements may be present within these sequences. 
ER  -

