
%0 Journal Article
%A Shao, X 
%A Kikuchi, K 
%A Watari, E 
%A Norose, Y 
%A Araki, T 
%A Yokomuro, K 
%T Changes in M-CSF-like activity during chicken embryonic development
%J Reproduction, Fertility and Development
%D 1996/02/01
%V 8
%N 1
%P 103-109
%K 
%R https://doi.org/10.1071/RD9960103
%U https://www.publish.csiro.au/paper/RD9960103 
%X We have established a method for separation of chicken bone-marrow cells using Percoll density gradient centrifugation, and have developed a new method for determining chicken M-CSF-like activity employing a liquid culture. Using this method, we determined M-CSF-like activities in egg yolk, chorioallantoic fluid (CAF) and amniotic fluid (AmF), and studied the effects of M-CSF on development of chicken embryos. M-CSF-like activity in egg yolk was at a high level before the incubation of the egg; it began to decrease on the third day of incubation and rapidly decreased on the fourth day, and no significant activity was detected after the tenth day of incubation. M-CSF-like activity in CAF was very low, and it exhibited almost no change during development. No M-CSF-like activity was detected in AmF throughout the experimental period. 
