
TY  - JOUR
A1  - Shao, X 
A1  - Kikuchi, K 
A1  - Watari, E 
A1  - Norose, Y 
A1  - Araki, T 
A1  - Yokomuro, K 
T1  - Changes in M-CSF-like activity during chicken embryonic development
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1996
VL  - 8
IS  - 1
SP  - 103
EP  - 109
KW  - 
UR  - https://doi.org/10.1071/RD9960103 
N2  - We have established a method for separation of chicken bone-marrow cells using Percoll density gradient centrifugation, and have developed a new method for determining chicken M-CSF-like activity employing a liquid culture. Using this method, we determined M-CSF-like activities in egg yolk, chorioallantoic fluid (CAF) and amniotic fluid (AmF), and studied the effects of M-CSF on development of chicken embryos. M-CSF-like activity in egg yolk was at a high level before the incubation of the egg; it began to decrease on the third day of incubation and rapidly decreased on the fourth day, and no significant activity was detected after the tenth day of incubation. M-CSF-like activity in CAF was very low, and it exhibited almost no change during development. No M-CSF-like activity was detected in AmF throughout the experimental period. 
ER  -

