
TY  - JOUR
A1  - Kusaka, M 
A1  - Sperelakis, N 
T1  - Stimulation of Ca2+ current by phorbol esters in rat myometrial cells is dependent on intracellular Ca2+ concentration
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 1996
VL  - 8
IS  - 8
SP  - 1147
EP  - 1152
KW  - 
UR  - https://doi.org/10.1071/RD9961147 
N2  - The effects of phorbol esters on the L-type Ca2+ current (ICa(L)) were investigated using nystatin-perforated patch and standard whole-cell voltage clamp in uterine smooth muscle cells isolated from late-pregnant rats. Using nystatin-perforated patch to maintain the integrity of the cytosol components, phorbol 12-myristate 13-acetate (PMA, 300 nM) increased ICa(L). When the standard whole-cell voltage clamp was used, the effect of PMA was dependent on the Ca2+ concentration in the pipette solution: PMA enhanced ICa(L) at pCa 6 and pCa 7 but not at pCa 10 or pCa 8. The effect of PMA was reversed by a selective inhibitor of protein kinase C, calphostin-C (500 nM). It is concluded that phorbol esters stimulate ICa(L) in uterine muscle cells and that the isoform of protein kinase C involved in this effect is Ca2+ dependent. This mechanism may be involved in the regulation of uterine contraction during pregnancy. 
ER  -

