
%0 Journal Article
%A Kim, U. H. 
%A Suh, G. H. 
%A Kim, I. H. 
%T 235 FOLLICULAR WAVE EMERGENCE AND DEVELOPMENT FOLLOWING ESTRADIOL PLUS PROGESTERONE AT A GROWING OR A STATIC PHASE OF THE DOMINANT FOLLICLE IN CIDR-TREATED, LACTATING DAIRY COWS
%J Reproduction, Fertility and Development
%D 2005/12/14
%V 18
%N 2
%P 225-226
%K 
%R https://doi.org/10.1071/RDV18N2AB235
%U https://www.publish.csiro.au/paper/RDV18N2AB235 
%X Treatment with estradiol benzoate (EB), compared to GnRH, in a controlled internal drug release (CIDR)-based timed AI protocol induced relatively asynchronous follicular wave emergence, resulting in ovulation of smaller ovulating follicles (Kim et al. 2005 Theriogenology 63, 260-268). In this study, we compared new follicular wave emergence and development following treatment with EB (2 mg) plus progesterone (50 mg) at a growing (Day 3 after ovulation) or a static (Day 8) phase of the dominant follicle during the first follicular wave in CIDR-treated, lactating dairy cows. Previously synchronized, lactating dairy cows received CIDRs (InterAg, Hamilton, New Zealand; Day 0) 3 days after ovulation and were assigned to two treatment groups and a control group. Cows in the T1 group (n = 11) received 2 mg EB (SY Esrone; Samyang, Seoul, Korea) and 50 mg P4 (SY Ovaron; Samyang) i.m., and those in the control group (n = 10) received no treatment at that time. Other previously synchronized cows received a CIDR (Day 0) with injections of 2 mg EB plus 50 mg P4 i.m. 8 days after ovulation (T2 group, n = 10). Thereafter, all cows received PGF2± (Lutalyse; Pharmacia &amp; Upjohn, Puurs, Belgium) at the time of CIDR removal on Day 7, and GnRH (Conceral; Dongbang Co., Seoul, Korea) on Day 9. Ovaries of each cow were examined by transrectal ultrasonography (Sonoace 600 with 7.5 MHz linear-array transducer; Medison Co., Ltd, Seoul, Korea) every 24 h from Days 0 to 9, on Day 11, and on Day 14 of treatment to observe changes in ovarian structures (follicles and corpora lutea (CL)). Data were analyzed by Fisher's exact test, Kruskal-Wallis test, and ANOVA using an SAS program (SAS Institute, Inc., Cary, NC, USA). Follicular wave emergence occurred within 7 days in 3/10 of the control group, 9/11 of the T1 group, and 9/10 of the T2 group (P < 0.05). The interval to wave emergence did not differ (P > 0.05) among groups (3.7 ± 1.2, 4.8 ± 0.5, 5.9 ± 0.3 days, respectively). While the diameters of dominant follicles on Day 0 were greater (P < 0.01) in the T2 group (14.3 ± 1.2 mm) than in the control (9.4 ± 0.6 mm) and T1 (10.5 ± 0.7 mm) group, diameters of preovulatory follicles on Day 9 were significantly smaller (P < 0.01) in the T1 (13.6 ± 0.7 mm) and T2 (12.3 ± 0.5 mm) groups than in the control group (16.4 ± 0.8 mm). Synchronized ovulation by 40 h after the GnRH injection (Day 11) occurred in 10/10 of the control group, 10/11 of the T1 group, and 9/10 of the T2 group (P > 0.05). In conclusion, administration of 2 mg EB plus 50 mg P4 at both a growing and a static phase of the dominant follicle during the first follicular wave results in synchronous follicular wave emergence and development and also synchronized ovulation in CIDR-treated, lactating dairy cows. 
