
TY  - JOUR
A1  - Camargo, L. S. A. 
A1  - Viana, J. H. M. 
A1  - Sa, W. F. 
A1  - Ferreira, A. M. 
A1  - Ramos, A. A. 
A1  - Freitas, C. 
A1  - Vale Filho, V. R. 
T1  - 272 DEVELOPMENTAL COMPETENCE OF OOCYTES OBTAINED FROM <i>BOS TAURUS</i> AND <i>BOS INDICUS</i> DAIRY COWS RAISED IN A TROPICAL CLIMATE
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 2005
VL  - 18
IS  - 2
SP  - 243
EP  - 244
KW  - 
UR  - https://doi.org/10.1071/RDV18N2AB272 
N2  - The effects of heat stress on Bos taurus reproductive performance in tropical and subtropical regions are well known, and have been associated with lower oocyte developmental capacity. The aim of this study was to evaluate the developmental competence of oocytes from Bos taurus (Holstein) and Bos indicus (Gyr) dairy cows raised in a Brazilian tropical region, located at 21&deg;35&prime;S latitude, 43&deg;51&prime;W longitude, and 435 meters altitude. Cumulus&ndash;oocyte complexes (COCs) were recovered by oocyte pickup (OPU) from mature non-lactating Holstein (n &equals; 9) and Gyr (n &equals; 13) donor cows between the end of spring and the beginning of autumn, with at least two OPU sessions/cow. COCs were in vitro-maturated in TCM-199 (GIBCO, Grand Island, NY, USA) with 10&percnt; inactivated estrus cow serum for 24 h under 5&percnt; CO2 at 38.5&deg;C in air. Bos taurus and Bos indicus semen with similar cleavage rates, previously evaluated by in vitro fertilization with oocytes obtained from slaughterhouse ovaries, were used to reduce bull effect. Holstein and Gyr spermatozoa were obtained through swim-up method and co-incubated with Holstein (n &equals; 390) and Gyr (n &equals; 505) oocytes, respectively, in Fert-TALP medium (Parrish et al. 1988 Biol. Reprod. 38, 1171&ndash;1180) supplemented with 10 &mu;g/mL heparin (Sigma-Aldrich, Sao Paulo, Brazil) and 6 mg/mL fatty acid-free bovine albumin (Sigma) for 18 h in 5&percnt; CO2 at 38.5&deg;C in air. Presumptive zygotes were co-cultured with their own cumulus cells in CR2aa medium (Wilkinson et al. 1996 Theriogenology 45, 41&ndash;49) supplemented with 10&percnt; fetal calf serum in humid atmosphere of 5&percnt; CO2 at 38.5&deg;C in air. On Day 7 to 8 of co-culture, Gyr and Holstein blastocysts were assessed and those classified as grade 1 (IETS Manual) were transferred to synchronized Bos indicus &times; Bos taurus crossbred recipients managed under the same nutritional and environmental conditions. Pregnancy diagnosis was performed between 35 and 50 days after estrus. Cleavage, blastocyst, and pregnancy rates were analyzed by chi-square test. Cleavage and blastocyst rates were greater (P < 0.05) in Gyr than in Holstein (66.7&percnt; vs. 53.1&percnt; for cleavage and 19.6&percnt; vs. 10.8&percnt; for blastocyst, respectively), but the pregnancy rate was similar (P > 0.05; 44.5&percnt; vs. 60&percnt; for Gyr and Holstein, respectively). These results show that Gyr oocytes obtained in a tropical region have greater developmental capacity than Holstein oocytes, suggesting an interaction between genotype and environment that influences the success of an in vitro embryo production program; nevertheless, the blastocyst viability after transferring to recipients is similar for both breeds. 
ER  -

