
TY  - JOUR
A1  - Galik, P. K. 
A1  - Gard, J. A. 
A1  - Spencer, T. S. 
A1  - Marley, M. S. 
A1  - Stringfellow, D. A. 
A1  - Givens, M. D. 
A1  - Edmondson, M. A. 
T1  - 153 EFFECTS OF OVINE INTERFERON-&#946; ON REPLICATION OF BOVINE VIRAL DIARRHEA VIRUS AND BOVINE HERPESVIRUS-1
JF  - Reproduction, Fertility and Development
JO  - Reprod. Fertil. Dev.
Y1  - 2007
VL  - 20
IS  - 1
SP  - 156
EP  - 157
KW  - 
UR  - https://doi.org/10.1071/RDV20N1AB153 
N2  - Bovine viral diarrhea virus (BVDV) and bovine herpesvirus-1 (BHV-1) are the most commonly isolated viruses from abattoir-origin materials utilized in embryo production and known to associate with zona pellucida-intact (ZP-I) embryos after exposure and washing. Some evidence indicates that developing embryos may produce substances that are able to inhibit viral replication in adjacent cells. Interferons such as recombinant human interferon-&#945; are known to have anti-BVDV activity but no effect against BHV-1. In some preliminary studies, bovine interferon-&#964; has shown antiviral activities against BVDV but not against BHV-1. However, interferon-&#964; in other species has not been evaluated for anti-BVDV and anti-BHV-1 effects. Thus, the objective of this study was to evaluate the cytotoxicity and anti-viral effect of ovine interferon-&#964; against a non-cytopathic high affinity strain of BVDV (SD-1) and BHV-1 (Colorado) in cell culture. Serial dilutions (1:10) beginning with an initial concentration of 1 mg mL&#8211;1 of interferon-&#964; were made in 96-well plates and then Madin Darby bovine kidney (MDBK) cells were seeded in the wells. Cells and interferon-&#964; were incubated at 37.5°C in 5% CO2 and air for 24 h prior to addition of virus. The following concentrations of BVDV were added to the wells: 6000, 3500, 1000, 625, and 350 cell culture infective doses (CCID50) (50% endpoint) per well. In addition, four viral concentrations of BHV-1, 1000, 500, 250, and 100 CCID50/mL were evaluated in separate cell cultures. Virus isolation was utilized to determine if the addition of interferon-&#964; decreased the amount of infective virus. Ovine interferon-&#964; produced no observable cytotoxicity in MDBK cells in any of the assays. Also, the three highest concentrations of interferon-&#964; significantly decreased the amount of BVDV in all of the concentrations of BVDV tested but had no apparent effect on the concentration of BHV-1 in cell cultures. Therefore ovine interferon-&#964; has anti-BVDV effects similar to those seen with bovine interferont-&#964; and neither has any apparent antiviral activity on BHV-1 in cell culture. Additionally, ovine and bovine interferon-&#964; might serve to limit or prevent the transmission of BVDV and curtail the negative effects of BVDV on oocyte and embryo development. However, a similar effect is not expected for BHV-1. 
ER  -

