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Vertebrate reproductive science and technology
RESEARCH ARTICLE

Vitrification of bovine germinal vesicle oocytes significantly decreased the methylation level of their in vitro derived MII oocytes

Peipei Zhang A # , Sha Yang A # , Hang Zhang A , Haisheng Hao A , Weihua Du A , Jingjing Wang A , Tong Hao A , Huabin Zhu A , Saqib Umer https://orcid.org/0000-0001-6110-2690 A and Xueming Zhao A *
+ Author Affiliations
- Author Affiliations

A Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), No. 2 Yuanmingyuan Western Road, Haidian District, Beijing 100193, China.

* Correspondence to: zhaoxueming@caas.cn
# These authors contributed equally to this paper

Handling Editor: Ye Yuan

Reproduction, Fertility and Development 34(13) 889-903 https://doi.org/10.1071/RD22130
Published online: 5 August 2022

© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

Context: The vitrification of oocytes is important for the conservation of animals, and the effect of vitrification on methylation patterns of bovine oocytes remains unclear.

Aims: This article aims to investigate the effect of vitrification on the DNA methylation patterns on vitrified GV oocytes and their in vitro derived MII oocytes.

Methods: 5-MeC staining and single-cell whole genome bisulphite sequencing (SC-WGBS) were utilised to analyse fresh GV oocytes (F_GV group), MII oocytes (F_MII group), vitrified GV oocytes (V_GV group) and their in vitro derived MII oocytes (V_MII group).

Key results: Results of both 5-MeC staining and SC-WGBS showed that no significant difference was found between the F_GV group and the V_GV group, while the methylation level of the V_MII group was significantly lower than that of the F_MII group. Moreover, supplementation of 2 μM resveratrol (Res) in IVM medium significantly improved maturation and development ability of vitrified GV oocytes by restoring their DNA methylation levels.

Conclusion: In conclusion, vitrification of bovine GV oocytes significantly decreased the DNA methylation level of their in vitro derived MII oocytes, and 2 μM Res improved their development ability by restoring DNA methylation level.

Implications: Our results provide an efficient approach to improve the maturation and fertilisation ability of vitrified GV oocytes.

Keywords: 5-MeC, bovine, DNA methylation, in vitro maturation, oocytes, resveratrol, SC-WGBS, vitrification.


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