
%0 Journal Article
%A Itoh, Masaaki 
%A Seo, Daisuke 
%A Sakurai, Hidehiro 
%A Setif, Pierre 
%T Electron transfer between soluble cytochrem c-554 and purified raction center from the green sulfur bacterium Chlorobium tepidum
%J Science Access
%D 2001/01/01
%V 3
%N 1
%P -
%K 
%R https://doi.org/10.1071/SA0403297
%U https://www.publish.csiro.au/paper/SA0403297 
%X Green sulfur bacteria contain three kinds of soluble cytochrome c (In Chlorobium limicola f. thiosulfatophilum; cytochrome c-553: flavocytochrome, c-551: e- acceptor of thiosulfate oxidoreductase (30 kDa), and c-555 (10 kDa), respectively). A reaction center (RC) of green sulfur bacteria also contains two molecules of monoheme cytochorme c. Photooxidized P840 is re-reduced by the reaction center-bound cytochorme c-551 with t1/2 of 60-110µs. The 10 kDa-cytochrome c was purified from the green sulfur bacterium Chlorobium tepidum. Its midpoint potential is 148mV. From its a-peak, it is referred to as cytochrome c-554. We measured electron transfer kinitics between purified cytochorme c-554 and RC of C. tepidum using single and multiple flash excitation measurements. The results indicate that cytochrome c-554 can reduce RC bound cytochrome c, but not directly P840. The rate of oxidation of cyt c-554 by oxidized RC was not strongly affected by the ionic strength of the medium. 
