
TY  - JOUR
A1  - Gilmore, S 
A1  - Weston, PH 
A1  - Thomson, JA 
T1  - A simple, rapid, inexpensive and widely applicable technique for purifying plant DNA
JF  - Australian Systematic Botany
JO  - Aust. Systematic Bot.
Y1  - 1993
VL  - 6
IS  - 2
SP  - 139
EP  - 148
KW  - 
UR  - https://doi.org/10.1071/SB9930139 
N2  - We describe and evaluate a quick and simple technique for purifying plant DNA from a wide range of taxa. The method utilises selective binding to diatomite in the presence of a chaotrope and allows simple, rapid and inexpensive preparation of DNA of length and purity adequate for restriction analysis of nuclear and chloroplast sites, cyclic DNA amplification and other studies. Coupled with recent developments in handling of field-collected plant samples and the development of non-radioactive nucleic acid labelling techniques, this purification protocol facilitates application of molecular systematic approaches in botanical institutions without access to specialised biochemical facilities. 
ER  -

