CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Australian Journal of Botany   
Australian Journal of Botany
Journal Banner
  Southern Hemisphere Botanical Ecosystems
blank image Search
blank image blank image
blank image
  Advanced Search

Journal Home
About the Journal
Editorial Board
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Turner Review Series
Sample Issue
For Authors
General Information
Notice to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our Email Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

red arrow PrometheusWiki
blank image
Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 23(2)

An Immunofluorescence Technique for Detecting Phytophthora cinnamomi Rands

N Malajczuk, AJ Mccomb and CA Parker

Australian Journal of Botany 23(2) 289 - 309
Published: 1975


Fluorescein-labelled antibodies were prepared for 'cell-wall' and 'soluble' antigens from P. cinnamomi. Those prepared against the cell wall fraction were superior for observing P. cinnamomi hyphae with the fluorescence microscope. Antibody-stained hyphae could be distinguished among hyphae from a wide range of fungal groups. P. cinnamomi hyphae could be seen on root surfaces and, although definition was hampered by plant tissue fluorescence, in infected roots. P. cinnamomi could not be detected in forest soil shown by baiting techniques to contain the pathogen, but was detected, and assayed quantitatively, in samples from laboratory soil cultures.

Full text doi:10.1071/BT9750289

© CSIRO 1975

blank image >
PDF (1.3 MB) $40
 Export Citation
Subscriber Login

Legal & Privacy | Contact Us | Help


© CSIRO 1996-2014