Factors Influencing the Paper Chromatography of Some Enzymes of Aspergillus Oryzae and Related Enzymes

Abstract

The influence of a number of variables on the paper chromatographic behaviour of eight enzymes (proteinase, amylase, catalase, esterase, acid phosphatase, p-nitrophenyl-,8-glucosidase, salicinase, and sucrase) present in the culture filtrate of A. oryzae has been investigated. The solvent s)'Stems used were mixtures of aqueous buffers and organic liquids. The nature and concentration of both the organic liquid and the buffer, pH, and the solute concentration strongly influence the results.