The Coupling of Cellobiase and Peroxidase by Glucose Oxidase

Abstract

Youatt (1958) has described a method for estimating cellobiase activity that uses the coupled glucose oxidase-catalase system to determine manometrically the amount of glucose liberated from cellobiose by the enzyme. The high specificity of glucose oxidase makes it the reagent of choice for the otherwise difficult determination of glucose in the presence of excess cellobiose. The hydrogen peroxide produced by glucose oxidase can, however, also be demonstrated qualitatively by the use of peroxidase and both Eyer, Linzenmeier, and von Schrader (1957) and Huggett and Nixon (1957) have described sensitive quantitative glucose oxidase-peroxidase systems for determining glucose as such.