The Effects of Some Anti-Oestrogens and Anti-Metabolites on the Binding of Tritiated Oestradiol by the Vagina of the Ovariectomized Mouse

Abstract

The antimetabolite actinomycin D inhibited the retention of [3H]oestradiol. 17 fJ and tetrazolium reduction in the mouse vagina when administered 1 and 25 hr before [3H]oestradiol. Inhibition of retention in both 105,000 g pellet and supernatant fractions of vaginal homogenates was observed with the pellet fraction more sensitive to the inhibitor. Actinomycin D inhibited tetrazolium. reduction, but not the retention of [3H]oestradiol, when administered 1 hr before [3H]oestradiol.