Effect of Composition of Tris-Based Diluent and of Thawing Solution on Survival of Ram Spermatozoa Frozen by the Pellet Method

Abstract

Six factorial experiments were conducted to examine the effects of concentra tion of tris(hydroxymethyl)aminomethane (Tris) and type and concentration of sugar in the freezing diluent, and of composition of thawing solution on the survival of ram spermatozoa following the freeze-thaw procedure.