Fertility Test of Frozen Boar Semen

Abstract

The fertility results of two experiments are presented. In experiment 1, the semen was frozen in tris-fructose-EDTA or BF3 diluents at 0'25xl09/mI sperm concentration and extended after thawing with either seminal plasma (SP) or the freezing medium (FM) containing no cryoprotective agent. In the second experiment the semen was glycerolated by two methods, frozen at 1· 0 x 109/ml sperm concentration, and extended with FM before insemination. Fertility after double insemination within one oestrus with semen frozen in tris-fructose-EDTA or BF 3 diluents varied depending on the medium used for extension of thawed semen. The farrowing rates for semen frozen in the former diluent with FM and SP post-thawing media were 4/8 and 1/8 respectively, and for semen frozen in BF3 diluent with FM and SP post-thawing extenders 1/8 and 5/8 resp_ectively. The mean farrowing for the 32 animals inseminated was 34·4 %. Pregnancies for semen frozen in tris-fructose-EDTA and glycerolated at 30 or 5°e were 5/12 and 4/12 respectively, and for single and double inseminations 6/12 and 3/12 respectively. Of 24 animals inseminated 37· 5 % farrowed.