Genetic diversity of the mungbean (Vigna radiata, Leguminosae) genepool on the basis of microsatellite analysis
Chontira Sangiri A C , Akito Kaga B C , Norihiko Tomooka B C , Duncan Vaughan B D and Peerasak Srinives AA Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73410, Thailand.
B National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki 305-8602, Japan.
C These authors contributed equally to this research.
D Corresponding author. Email: duncan@affrc.go.jp
Australian Journal of Botany 55(8) 837-847 https://doi.org/10.1071/BT07105
Submitted: 4 June 2007 Accepted: 27 August 2007 Published: 14 December 2007
Abstract
A large representative collection of mungbean (Vigna radiata (L.) Wilczek) consisting of 415 cultivated, 189 wild and 11 intermediate accessions was analysed by using 19 SSR primers. These SSR primers showed polymorphism in wild and cultivated mungbean and were selected from those available for the related species azuki bean [V. angularis (Willd.) Ohwi & Ohwi]. One or more SSR primer for each linkage group (on the basis of the azuki linkage map) was analysed. In total, 309 alleles were detected and of these, about twice as many were detected in wild (257 alleles) as in cultivated accessions (138 alleles). The results show that Australia and New Guinea represent a distinct centre of diversity for wild mungbean. Cultivated mungbean has greatest diversity in South Asia, which supports the view that South Asia is where this crop was domesticated. SSR marker allelic diversity for cultivated mungbean has distinct regional variation, with high variation in South and West Asia. The present study represents the first comprehensive analysis of wild and cultivated mungbean germplasm diversity by SSR markers, and highlights specific genetic diversity that might be used to broaden the genetic base of currently grown mungbean cultivars.
Acknowledgements
A Royal Golden Jubilee PhD Programme Grant to author C. S. and a Basic Research Grant from the Thailand Research Fund to author P. S. are gratefully acknowledged. We thank the following organisations for providing germplasm used in the study: the Australian Plant Genetic Resources Information Service, Queensland, Australia; the National Botanic Garden of Belgium, Meise, Belgium; the International Center for Tropical Agriculture (CIAT), Colombia; the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany; the International Board for Plant Genetic Resource, Rome, Italy; the National Institute of Agrobiological Science, Tsukuba, Japan; the World Vegetable Center (AVRDC), Taiwan, China; Chai Nat Field Crops Research Centre, Chai Nat, Thailand; National Center for Genetic Engineering and Biotechnology, Kasetsart University, Kampaeng Saen, Thailand; and the National Plant Germplasm System, United States Department of Agriculture, USA.
Bisht IS,
Bhatl KV,
Lakhanpaul S,
Latha M,
Jayan PK,
Biswas BK, Singh AK
(2005) Diversity and genetic resources of wild Vigna species in India. Genetic Resources and Crop Evolution 52, 53–68.
| Crossref | GoogleScholarGoogle Scholar |
[Verified 7 October 2007].
Wang XW,
Kaga A,
Tomooka N, Vaughan DA
(2004) The development of SSR markers by a new method in plants and their application to gene flow studies in azuki bean [Vigna angularis (Willd.) Ohwi and Ohashi]. Theoretical and Applied Genetics 109, 352–360.
| PubMed |
Zhu Q,
Zheng X,
Luo J,
Gaut BS, Ge S
(2007) Multilocus analysis of nucleotide variation of Oryza sativa and its wild relatives: severe bottleneck during domestication of rice. Molecular Biology and Evolution 24, 875–888.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
