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RESEARCH ARTICLE

Typing of Plum pox virus (PPV) strains in Cyprus

L. C. Papayiannis A B , A. Kyriakou A and T. Kapari-Isaia A
+ Author Affiliations
- Author Affiliations

A Agricultural Research Institute, PO Box 22016, Nicosia 1516, Cyprus.

B Corresponding author. Email: l.papayiannis@arinet.ari.gov.cy

Australasian Plant Disease Notes 2(1) 29-30 https://doi.org/10.1071/DN07013
Submitted: 6 December 2007  Accepted: 5 March 2007   Published: 29 March 2007

Abstract

A survey was conducted in the main stone fruit producing areas of Cyprus during 2004–05 in order to determine the identity and distribution of Plum pox virus strains using serological and molecular methods. A total of 72 peach, apricot and plum tree samples were analysed by ELISA and RT-PCR and results showed that PPV-M was the predominant strain, while PPV-D was identified for the first time in the island.


References


Atanasoff D (1935) Mosaic of stone fruits. Phytopathologie Zeitschrift 8, 259–284. open url image1

Boscia D, Zeramdini H, Cambra M, Potore O, Gorris MT, Myrta A, Di Terlizzi B, Savino V (1997) Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus. European Journal of Plant Pathology 103, 477–480.
Crossref | GoogleScholarGoogle Scholar | open url image1

Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme linked immunosorbent assay for the detection of plant viruses. The Journal of General Virology 34, 475–483.
PubMed |
open url image1

Kernal C, Dunez J (1979) Differentiation biologique et serologique des souches du virus de la sharka. Annals de Phytopathologie 11, 541–555. open url image1

Labonne G, Yvon M, Quiot JB, Avinent L, Llácer G (1995) Aphids as potential vectors of plum pox virus: Comparison of methods of testing and epidemiological consequences. Acta Horticulturae 386, 207–218. open url image1

Myrta A, Di Terlizzi B, Boscia D, Caglayan K, Gavriel I, Ghanem G, Varveri C, Savino V (1998) Detection and serotyping of Mediterranean plum pox virus isolates by means of strain-specific monoclonal antibodies. Acta Virologica. English Ed. 42, 251–254. open url image1

Navratil M, Safarova D, Karesova R, Petrzik K (2005) First incidence of Plum pox virus on apricot trees in China. Plant Disease 89, 338. open url image1

Olmos A, Cambra M, Dasi MA, Candresse T, Esteban O, Gorris MT, Asensio M (1997) Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA. Journal of Virological Methods 68, 127–137.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Spiegel S, Kovalenko EM, Varga A, James D (2004) Detection and partial molecular characterization of two Plum pox virus isolates from plum and wild apricot in southeast Kazakhstan. Plant Disease 88, 973–979. open url image1

Stobbs LW, Van Driel L, Whybourne K, Carlson C, Tulloch M, Van Lier J (2005) Distribution of Plum pox virus in residential sites, commercial nurseries and native plant species in the Niagara Region, Ontario, Canada. Plant Disease 89, 822–827. open url image1

Wetzel T, Candresse T, Ravelonandro M, Dunez J (1991) A polymerase chain reaction adapted to plum pox potyvirus detection. Journal of Virological Methods 33, 355–365.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1