Natural infection of periwinkle (Catharanthus roseus) with Cucumber mosaic virus, subgroup IB

A. Samad; P. V. Ajayakumar; M. K. Gupta; A. K. Shukla; M. P. Darokar; B. Somkuwar; M. Alam

doi:10.1071/DN08013

RESEARCH ARTICLE

Natural infection of periwinkle (Catharanthus roseus) with Cucumber mosaic virus, subgroup IB

A. Samad ^A ^B , P. V. Ajayakumar ^A , M. K. Gupta ^A , A. K. Shukla ^A , M. P. Darokar ^A , B. Somkuwar ^A and M. Alam ^A

+ Author Affiliations

- Author Affiliations

^A Central Institute of Medicinal and Aromatic Plants (CIMAP), P.O., CIMAP, Lucknow-226 015, India.

^B Corresponding author: Email: samad_cimap@yahoo.co.in

Australasian Plant Disease Notes 3(1) 30-34 https://doi.org/10.1071/DN08013
Submitted: 30 January 2008 Accepted: 19 March 2008 Published: 9 April 2008

Abstract

This paper reports the characterisation of a naturally occurring Cucumber mosaic virus (CMV) infection, causing mosaic, leaf distortion and stunting of periwinkle (Catharanthus roseus). The virus isolate showed wide host range, biophysical properties similar to CMV, isometric particles of 28 nm, capsid protein of 26 kDa, serological reaction with CMV-S and non-persistent transmission by aphids. The CP gene of the virus was amplified using reverse transcriptase–polymerase chain reaction (RT-PCR) with CP-specific primers, cloned and sequenced (657 bp). Sequence analysis of the PCR product with other CMV isolates revealed the closest identity with Rauvolfia serpentina isolate of CMV (98%) and the phylogram revealed that CMV naturally infecting periwinkle belongs to subgroup IB.

Acknowledgements

The authors are thankful to Dr S.P.S. Khanuja (Director, CIMAP) for encouragement and providing necessary facilities. Thanks also to Dr AK Gupta, National Gene Bank of Medicinal and Aromatic Plants, CIMAP, Lucknow for providing the germplasm of periwinkle (C. roseus) for this study.

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