First report of Tomato yellow leaf curl China virus with DNA β infecting Datura stramonium in China
M. Ding A , Y. Q. Luo A , J. H. Dong A , Q. Fang A and Z. K. Zhang A BA Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences, Kunming 650223, China.
B Corresponding author. Email: zhongkai99@sina.com
Australasian Plant Disease Notes 2(1) 63-63 https://doi.org/10.1071/DN07026
Submitted: 12 October 2006 Accepted: 10 April 2007 Published: 19 April 2007
Abstract
Tomato yellow leaf curl China virus, associated with a satellite DNA β, has been detected in Datura stramonium (jimsonweed) with leaf curl disease for the first time in China.
A virus isolate (YN72) was collected from Datura stramonium (jimsonweed) showing chlorotic and leaf curl symptoms (Fig. 1) from Binchuan in the Yunnan province of China in July 2005. The isolate gave a positive reaction with antiserum to African cassava mosaic virus using DAS-ELISA (AGDIA, Elkhart, IN). Total DNA was extracted and tested for the presence of geminiviral DNA using polymerase chain reaction (PCR) with the begomovirus-specific degenerate primer pair PA/PB (Deng et al. 1994). PCR products of the expected size (~500 bp) were obtained, cloned into pGEM-T Easy (Promega, Madison, WI), and sequenced (GenBank Accession EF011561). Nucleotide sequence comparison showed 99% identity with Malvastrum yellow vein Yunnan virus (isolate Y160) (GenBank Accession AJ971500). The remaining DNA-A sequence of YN72 was amplified by PCR using the primers of Dong et al. (2007). The complete DNA-A sequence of YN72 was determined to be 2739 nucleotides (GenBank Accession EF011559). Comparisons with viral sequences available at the GenBank-EMBL databases using BLASTN showed that DNA-A of YN72 is closely related to Tomato yellow leaf curl China virus-(Y43) with 96.7% nucleotide sequence identity.
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To test whether a satellite molecule was associated with this isolate, a universal primer pair specific for DNA β was used to amplify the putative DNA (Briddon et al. 2002). Using this primer set, an amplification product of ~1300 bp was obtained. Sequence analysis revealed that this DNA β of YN72 was 1335 nucleotides long (GenBank Accession EF011560) and most closely related to the DNA β associated with Tomato yellow leaf curl China virus-(Y254) (GenBank Accession AJ971332; 98% nucleotide sequence identity).
To our knowledge, this is the first report of a begomovirus, which has DNA A along with a satellite DNA β, being associated with leaf curl disease on Datura stramonium in China.
Acknowledgements
This work was funded by the National Natural Science Foundation of China (Grant No.30360005) and the Natural Science Foundation of Yunnan Province, China (Grant No. 2005C0064M, 2005C0012Z).
Briddon RW,
Bull SE,
Mansoor S,
Amin I, Markham PG
(2002) Universal primers for the PCR-mediated amplification of DNA β; a molecule associated with some monopartite begomoviruses. Molecular Biotechnology 20, 315–318.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Deng D,
McGrath PF,
Robinson DJ, Harrison BD
(1994) Detection and differentiation of whitefly-transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. The Annals of Applied Biology 125, 327–336.
Dong JH,
Luo YQ,
Ding M,
Zhang ZK, Yang CK
(2007) First report of Tomato yellow leaf curl China virus infecting kidney bean in China. Plant Pathology 56, 342–
.
| Crossref | GoogleScholarGoogle Scholar |
