Australian Journal of Botany Australian Journal of Botany Society
Southern hemisphere botanical ecosystems
RESEARCH ARTICLE

Basic chromosome number in Boronia (Rutaceae)—competing hypotheses examined

Fucheng Shan A B , Guijun Yan A and Julie A. Plummer A

A School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

B Corresponding author. Present address: Innovative Plant Products Group, Department of Agriculture and Food of Western Australia, 3 Baron Hay Court, Perth, WA 6151, Australia. Email: fshan@agric.wa.gov.au

Australian Journal of Botany 54(7) 681-689 http://dx.doi.org/10.1071/BT05050
Submitted: 4 March 2005  Accepted: 18 May 2006   Published: 19 October 2006

Abstract

Rutaceae have attracted considerable attention because of the wide chromosome-number variation. Cytoevolution of the genus Boronia, with n = 7–36, has been controversial. The critical issue is whether the base chromosome number is x = 18 or x = 9 in this genus and in the family Rutaceae. Phylogenetic analysis based on random amplified polymorphic DNA (RAPD) markers was used to evaluate the hypothesis. Twenty decamer arbitrary primers were used to produce RAPD markers in 25 accessions of 18 Boronia species and a total of 559 DNA fragments was generated. UPGMA distance analysis and Wagner parsimony analysis on the DNA data produced two phylogenetic trees with very similar topology. The two trees generally supported the present classification of Boronia species. The exception was B. tenuis, which may be better treated as a new section or genus. Chromosome numbers of all the genotypes used in the analysis were counted with n = 7, 8, 9, 11, 16–36. Evolutionary distances between species were determined on the basis of branch length of the Wagner cladogram. Regression analysis indicated that Boronia chromosome number has a significant negative relationship with evolutionary distance. Chromosome number in Boronia evolved from higher to lower. The basic chromosome number for Boronia is suggested to be 18.


References

Ahmad M McNeil DL 1996 Comparison of crossability, RAPD, SDS–PAGE and morphological markers for revealing genetic relationships within and among Lens species. Theoretical and Applied Genetics 93 788 793 doi:10.1007/BF00224077

Armstrong JA 1979 Biotic pollination mechanisms in the Australian flora. New Zealand Journal of Botany 17 467 508

Armstrong JA 1987 Floral syndromes as generic determinants. Australian Systematic Botany Society Newsletter 53 54 59


Astarini IA Yan G Plummer JA 1999 Interspecific hybridisation of boronias. Australian Journal of Botany 47 851 864
doi:10.1071/BT98017

Bentham G (1863) ‘Flora Australiensis.’ (Lovell Reeve: London)

Caetano-Anolles G Bassam BJ Gresshoff PM 1991 DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Bio/Technology 9 553 557 doi:10.1038/nbt0691-553

Chase MW Morton CM Kallunki JA 1999 Phylogenetic relationships of Rutaceae: a cladistic analysis of the subfamilies using evidence from rbcL and atpB sequence variation. American Journal of Botany 86 1191 1199 doi:10.2307/2656983

da Silva MFDGF Gottlieb OR Ehrendorfer F 1988 Chemosystematics of the Rutaceae: suggestions for a more natural taxonomy and evolutionary interpretation of the family. Plant Systematics and Evolution 161 97 134 doi:10.1007/BF00937293

Darlington CD , Wylie AP (1955) ‘Chromosome atlas of flowering plants.’ (George Allen and Unwin: London)

Engler A , Prantl K (1896) ‘Die Naturlichen Pfanzenfamilien.’ (Engelmann: Leipzig, Germany)

Federici CT Fang DQ Scora RW Roose ML 1998 Phylogenetic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis. Theoretical and Applied Genetics 96 812 822 doi:10.1007/s001220050807

Federov A (1969) ‘Chromosome numbers of flowering plants.’ (Nauk: Leningrad, Russia)

Felsenstein J 1985 Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783 791 doi:10.2307/2408678

Goodwin PH Annis SL 1991 Rapid identification of genetic variation and pathotype of Leptosphaeria maculans by random amplified polymorphic DNA assay. Applied and Environmental Microbiology 57 2482 2486

Grant V 1982 Periodicities in the chromosome numbers of the angiosperms. Botanical Gazette 143 379 389
doi:10.1086/337313

Henry RJ (1997) ‘Practical applications of plant molecular biology.’ (Chapman and Hall: London)

Hnatiuk RJ (1990) ‘Census of Australian vascular plants, vol. 11.’ (Australian Government Publishing Service: Canberra)

Landry BS Li RQ Cheung WY Granger RL 1994 Phylogeny analysis of 25 apple rootstocks using RAPD markers and tactical gene tagging. Theoretical and Applied Genetics 89 847 852 doi:10.1007/BF00224507

Lazaro A Aguinagalde I 1996 Phylogenetic relationships between the wild taxa of the Brassica oleracea L. group (2n = 18) using random amplified polymorphic DNA assay. Scientia Horticulturae 65 219 227 doi:10.1016/0304-4238(96)00894-1

Martin GB Williams JGK Tanksley SD 1991 Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines. Proceedings of the National Academy of Sciences of the USA 88 2336 2340 doi:10.1073/pnas.88.6.2336

Millan T Osuna F Cobos S Torres AM Cubero JI 1996 Using RAPDs to study phylogenetic relationships in Rosa. Theoretical and Applied Genetics 92 273 277 doi:10.1007/BF00223385

Nei M (1987) ‘Molecular evolutionary genetics.’ (Columbia University Press: New York)

Nei M , Kumar S (2000) ‘Molecular evolution and phylogenetics.’ (Oxford University Press: Oxford, UK)

Rath P Rajaseger G Goh CJ Kumar PP 1998 Phylogenetic analysis of Dipterocarpus using random amplified polymorphic DNA markers. Annals of Botany 82 61 65 doi:10.1006/anbo.1998.0652

Raven PH 1975 The basis of angiosperm phylogeny: cytology. Annals of the Missouri Botanical Garden 62 724 764 doi:10.2307/2395272

Rogers SO Bendich AJ 1994 Extraction of total cellular DNA from plants, algae and fungi. Plant Molecular Biology Manual D1 1 8

Sambrook J , Fritsch EF , Maniatis T (1989) ‘Molecular cloning: a laboratory mannual.’ (Cold Spring Harbor: New York)

Samec P Nasinec V 1996 The use of RAPD technique for the identification and classification of Pisum sativum L. genotypes. Euphytica 89 229 234
doi:10.1007/BF00034610

Shan F Yan G Plummer JA 2003 a Cytoevolution of Boronia genome revealed by flourescent in situ hybridisation with rDNA probes. Genome 46 507 513

Shan F Yan G Plummer JA 2003 b Karyotype evolution in the genus Boronia (Rutaceae). Botanical Journal of the Linnean Society 142 309 320 doi:10.1046/j.1095-8339.2003.00163.x

Shan F Yan G Plummer JA 2003 c Meiotic chromosome behaviour and Boronia (Rutaceae) genome reorganization. Australian Journal of Botany 51 599 607 doi:10.1071/BT03042

Smith-White S 1954 Chromosome numbers in the Boronieae (Rutaceae) and their bearing on the evolutionary development of the tribe in the Australian flora. Australian Journal of Botany 2 287 303 doi:10.1071/BT9540287

Stace HM (1995) Primitive and advanced character states for chromosome number in Gondwanan angiosperm families of Australia, especially Rutaceae and Proteaceae. In ‘Kew chromosome conference IV’. (Eds PE Brandham, MD Bennett) pp. 223–232. (Royal Botanic Gardens: Kew, UK)

Stace HM Armstrong JA 1992 New chromosome numbers for Rutaceae. Australian Systematic Botany 5 501 505 doi:10.1071/SB9920501

Stace HM Patrick SJ 1993 Cytological notes in Rutaceae: 1. Boronia tenuis. Nuytsia 9 131 133

Stace HM Armstrong JA James SH 1993 Cytoevolutionary patterns in Rutaceae. Plant Systematics and Evolution 187 1 28
doi:10.1007/BF00994089

Sunglow Flowers Pty Ltd 1990 Boronia (Boronia heterophylla). Varieties ‘Moonglow’ and ‘Cameo’. Plant Varieties Journal 3 25 26

Swofford DL (2002) ‘PAUP: phylogenetic analysis using parsimony (and other methods), version 4.0b10.’ (Sinaur Associates: Sunderland, MA)

Welsh J McClelland M 1990 Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 18 7213 7218


Weston PH Carolin RC Armstrong JA 1984 A cladistic analysis of Boronia Sm. and Boronella Baill. (Rutaceae). Australian Journal of Botany 32 187 203
doi:10.1071/BT9840187

Wilkie SE Isaac PG Slater RJ 1993 Random amplified polymorphic DNA (RAPD) markers for genetic analysis in Allium. Theoretical and Applied Genetics 86 497 504 doi:10.1007/BF00838566

Williams JGK Kubelik AR Livak KJ Rafalski JA Tingey SV 1990 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18 6531 6535

Wilson PG 1971 Taxonomic notes on the family Rutaceae, principally of Western Australia. Nuytsia 1 197 207


Wilson PG 1998 New names and new taxa in the genus Boronia (Rutaceae) from Western Australia, with notes on seed characters. Nuytsia 12 119 154


Yan G Shan F Plummer JA 2002 Genomic relationships within Boronia (Rutaceae) as revealed by karyotype analysis and RAPD molecular markers. Plant Systematic Evolution 233 147 161




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