Leaf anatomy and histochemistry of Lippia citriodora (Verbenaceae)
Catherine Argyropoulou A , Anastasia Akoumianaki-Ioannidou B , Nikolaos S. Christodoulakis C and Costas Fasseas A DA Electron Microscopy Laboratory, Department of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Hellas (Greece).
B Laboratory of Floriculture and Landscape Architecture, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Hellas (Greece).
C Faculty of Biology, Department of Botany, University of Athens, 15701 Athens, Hellas (Greece).
D Corresponding author. Email: cfass@aua.gr
Australian Journal of Botany 58(5) 398-409 https://doi.org/10.1071/BT10072
Submitted: 20 March 2010 Accepted: 20 May 2010 Published: 21 July 2010
Abstract
Lippia citriodora H.B.K. is an aromatic plant indigenous to South America. It is cultivated and commercialised as an ornamental for its lemon-like scent emitted from its leaves and flowers. The present morphoanatomical and histochemical study revealed that leaves of L. citriodora possess one type of setae (non-glandular) and at least five types of glandular trichomes, with the latter differing anatomically and in the composition of their secondary metabolites. Scanning electron microscopy revealed that the same types of trichomes exist also on the calyces. Histochemical tests indicate that the glandular trichomes contain a combination of terpenoids, flavonoids, carbohydrates, phenolics and alkaloids. However, within the vacuoles of the mesophyll cells, terpenoids, phenolics, flavonoids and tannins are stored. The presence of lamellar cytoplasmic inclusions in the mesophyll cells, possibly precursors of tannins stored in the vacuoles, is reported for the first time.
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