Australian Journal of Botany Australian Journal of Botany Society
Southern hemisphere botanical ecosystems
RESEARCH ARTICLE

Anatomical characterisation of the foliar colleters in Myrtoideae (Myrtaceae)

Cleber J. da Silva A , Luiz Cláudio de A. Barbosa B , Ana E. Marques C , Maria Cristina Baracat-Pereira C , Antônio L. Pinheiro D and Renata M. S. A. Meira A E
+ Author Affiliations
- Author Affiliations

A Universidade Federal de São João Del-Rei, Campus de Sete Lagoas, 35701-970 Sete Lagoas, MG, Brazil.

B Departamento de Química, Universidade Federal de Viçosa, 36571-000 Viçosa, MG, Brazil.

C Departamento de Bioquímica e Biologia Molecular, Universidade Federal Viçosa, 36571-000 Viçosa, MG, Brazil.

D Departamento de Engenharia Florestal, Universidade Federal de Viçosa, 36571-000 Viçosa, MG, Brazil.

E Corresponding author. Email: rmeira@ufv.br

Australian Journal of Botany 60(8) 707-717 https://doi.org/10.1071/BT12149
Submitted: 6 June 2012  Accepted: 15 October 2012   Published: 30 November 2012

Abstract

Colleters are secretory structures that occur in vegetative or reproductive shoot apices of many botanical families. However, in the order Myrtales, reports of colleters have considered only external morphology. We therefore evaluated apical meristems of 52 species belonging to 17 genera from seven tribes of subfamily Myrtoideae (Myrtaceae), so as to analyse the incidence and morphological types of colleters. The samples were fixed for light and scanning electron microscopy. Histochemical tests were carried out on fresh and methacrylate-embedded material. Proteins of the colleter secretions were analysed by SDS-PAGE. We have classified and described the following three new colleter types: petaloid, conic and euryform. None of the species contained all three colleter types. The petaloid colleters were present in three tribes (Syzygieae, Melaleuceae and Lophostemoneae). The conic colleters were observed in three tribes (Leptospermeae, Myrteae and Melaleuceae) and the euryform type occurred in five tribes (Leptospermeae, Syncarpieae, Myrteae, Syzygieae and Melaleuceae). In the tribe Eucalypteae, we found no evidence of colleters. The presence of mucilaginous secretion that defines colleters was confirmed by histochemical tests, and no proteins were found in the secretion. The colleters in Myrtoideae may help clarify the phylogenetic relationships of the Myrtaceae family.

Additional keywords: colleter types, histochemistry, polysaccharide secretions, vegetative organs.


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