Australian Journal of Botany Australian Journal of Botany Society
Southern hemisphere botanical ecosystems
RESEARCH ARTICLE

Carbohydrate mobilisation in germinating seed of Enterolobium contortisiliquum and Peltophorum dubium (Fabaceae), two tropical trees used for restoration

Marina Belloni Veronesi A , Kelly Simões A , Nelson Augusto dos Santos-Junior B and Marcia Regina Braga A C

A Plant Physiology and Biochemistry Department, Institute of Botany, PO Box 68041, São Paulo, 04045-972, SP, Brazil.

B Seed Department, Institute of Botany, PO Box 68041, São Paulo, 04045-972, SP, Brazil.

C Corresponding author. Email: bragamr@ig.com.br

Australian Journal of Botany 62(2) 132-140 http://dx.doi.org/10.1071/BT13242
Submitted: 4 October 2013  Accepted: 31 March 2014   Published: 12 May 2014

Abstract

Enterolobium contortisiliquum (Vell.) Morong. and Peltophorum dubium (Spreng.) are two leguminous species native to Brazil that are frequently used to restore degraded areas. Seed of E. contortisiliquum are exalbuminous, whereas seed of P. dubium have a mucilaginous endosperm and both are orthodox, dormant and have a water-impermeable seed coat. There is little information about the dynamics of their germination and understanding this process is important for propagation, conservation and satisfactory practices for restoration of degraded areas. Thus, in this study we evaluated and compared the carbohydrate mobilisation of the seed of both species during germination and early seedling development. Data obtained showed differences in the composition and in the mobilisation of the storage carbohydrates in the studied species. Whereas the main storage of the E. contortisiliquum embryo is starch, the main reserve found in P. dubium is the galactomannan stored in the endosperm. The carbohydrates first hydrolysed in both species are raffinose family oligosaccharides that are used in the embryo development during germination. Starch found in cotyledons of E. contortisiliquum or accumulated after galactomannan degradation in the embryo of P. dubium is not used during germination and early seedling growth in either species.

Additional keywords: galactomannan, legume, newly germinated seed, raffinose-family oligosaccharides, starch.


References

Amaral LIV, Gaspar M, Costa PMF, Aidar MPM, Buckeridge MS (2007) Novo método enzimático rápido e sensível de extracão e dosagem de amido em materiais vegetais. Hoehnea 34, 425–431.
Novo método enzimático rápido e sensível de extracão e dosagem de amido em materiais vegetais.CrossRef | open url image1

Aquino AFMAG, Ribeiro MCC, Paula YMC, Bnedito CP (2009) Superação de dormência em sementes de orelha-de-negro (Enterolobium contortisiliquum (Vell.) Morang.). Revista Verde de Agrologia e Desenvolvimento Sustentável 4, 69–75.

Assis IR, Dias LE, Abrahão WAP, Ribeiro Junior ES, Mello JWV (2011) Cover layers to the growth of trees and shrubs over a sulfide spoil from gold mining. Revista Árvore 35, 941–947.
Cover layers to the growth of trees and shrubs over a sulfide spoil from gold mining.CrossRef | open url image1

Bernal-Lugo I, Leopold CA (1992) Changes in soluble carbohydrates during seed storage. Plant Physiology 98, 1207–1210.
Changes in soluble carbohydrates during seed storage.CrossRef | 1:CAS:528:DyaK38XhvFWiurY%3D&md5=8c2c2af175182706ee1859346a1642a2CAS | 16668748PubMed | open url image1

Bewley JD, Black M (1994) ‘Seeds: physiology of development and germination.’ 2nd edn. (Plenum Press: New York)

Bewley JD, Leung DWM, Maclsaac S, Reid JSG, Xu N (1993) Transient starch accumulation in the cotyledons of fenugreek seeds during galactomannan mobilization from the endosperm. Plant Physiology and Biochemistry 31, 483–490.

Black CM, Corbineau F, Gee H, Come D (1999) Water content, raffinose and dehydrins in the induction of desiccation tolerance in immature wheat embryos. Plant Physiology 120, 463–472.
Water content, raffinose and dehydrins in the induction of desiccation tolerance in immature wheat embryos.CrossRef | 1:CAS:528:DyaK1MXktFWqsr4%3D&md5=05ae79a8e12564555b35b4fe20c3aefbCAS | open url image1

Buckeridge MS (2010) Seed cell wall storage polysaccharides: models to understand cell wall biosynthesis and degradation. Plant Physiology 154, 1017–1023.
Seed cell wall storage polysaccharides: models to understand cell wall biosynthesis and degradation.CrossRef | 1:CAS:528:DC%2BC3cXhsV2nsbfN&md5=0016c04e11e2e36ec0a71b2f501bc383CAS | 20855518PubMed | open url image1

Buckeridge MS, Dietrich SMC (1990) Galactomannan from Brazilian legume seeds. Revista Brasileira de Botânica 13, 109–112.

Buckeridge MS, Dietrich SMC (1996) Mobilisation of the raffinose family oligosaccharides and galactomannan in germinating seeds of Sesbania marginata Benth. (Leguminosae–Faboideae). Plant Science 117, 33–43.
Mobilisation of the raffinose family oligosaccharides and galactomannan in germinating seeds of Sesbania marginata Benth. (Leguminosae–Faboideae).CrossRef | 1:CAS:528:DyaK28Xjt1yru7k%3D&md5=27abc4e844d670def6e20b0453ff9968CAS | open url image1

Buckeridge MS, Aidar MPM, Santos HP, Tiné MAS (2004) Acúmulo de reservas. In ‘Germinação: do básico ao aplicado’. (Eds AG Ferreira, F Borghetti) pp. 31–50. (Artmed: Porto Alegre, Brazil)

Carvalho CP, Hayashi AH, Braga MR, Nievola CC (2013) Biochemical and anatomical responses related to the in vitro survival of the tropical bromeliad Nidularium minutum to low temperatures. Plant Physiology and Biochemistry 71, 144–154.
Biochemical and anatomical responses related to the in vitro survival of the tropical bromeliad Nidularium minutum to low temperatures.CrossRef | 1:CAS:528:DC%2BC3sXhsFensLfL&md5=62b6d6aaf11bc87a982e6cfc8d9af076CAS | 23917072PubMed | open url image1

Costa MP, Nappo ME, Caçador FRD, Barros HHD (2010) Avaliação do processo de reabilitação de um trecho de floresta ciliar na Bacia do Rio Itapemirim-ES. Revista Árvore 34, 835–841.
Avaliação do processo de reabilitação de um trecho de floresta ciliar na Bacia do Rio Itapemirim-ES.CrossRef | open url image1

Davide AC, Silva EAA (Eds) (2008) ‘Produção de sementes e mudas de espécies florestais.’ (Universidade Federal de Lavras: Lavras, Brazil)

Davide AC, Faria JMR, Botelho SA (1995) ‘Propagação de espécies florestais.’ (Companhia Energética de Minas Gerais: Belo Horizonte, Brazil)

Dirk LMA, van der Krol AR, Vreugdenhil D, Hilhorst HWM, Bewley JD (1999) Galactomannan, soluble sugar, and starch mobilization following germination of fenugreek (Trigonella foenum-graecum L.) seeds. Plant Physiology Biochemistry 37, 41–50.
Galactomannan, soluble sugar, and starch mobilization following germination of fenugreek (Trigonella foenum-graecum L.) seeds.CrossRef | 1:CAS:528:DyaK1MXhsFyqsr8%3D&md5=a06875f1c0f7d764903db3ce437e645dCAS | open url image1

Donadio NMS, Demattê MESP (2000) Morfologia de frutos, sementes e plântulas de canafístula (Peltophorum dubium (Spreng.) Taub) e jacarandá-da-Bahia (Dalbergia nigra (Vell.) Fr.All. ex Benth) – Fabaceae. Revista Brasileira de Sementes 22, 64–73.

Downie B, Bewley D (2000) Soluble sugar content of white spruce (Picea glauca) seeds during and after germination. Physiologia Plantarum 110, 1–12.
Soluble sugar content of white spruce (Picea glauca) seeds during and after germination.CrossRef | 1:CAS:528:DC%2BD3cXms1Cquro%3D&md5=6a951d867ad180b618503a11c0657346CAS | open url image1

Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350–356.
Colorimetric method for determination of sugars and related substances.CrossRef | 1:CAS:528:DyaG28XjvFynsg%3D%3D&md5=168f1302597d4399b76eaee53115fa07CAS | open url image1

Durigan G, Nishikawa DLL, Rocha E, Silveira ER, Pulitano FM, Regalado LB, Carvalhaes MA, Paranaguá PA, Ramilu VEL (2002) Caracterização de dois estados da vegetação de uma área de cerrado no município de Brotas-SP, Brasil. Acta Botânica Brasílica 16, 251–262.
Caracterização de dois estados da vegetação de uma área de cerrado no município de Brotas-SP, Brasil.CrossRef | open url image1

Ferreira DF (2011) Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia 35, 1039–1042.
Sisvar: a computer statistical analysis system.CrossRef | open url image1

Ferreira AG, Borghetti F (Eds) (2004) ‘Germinação: do básico ao aplicado.’ (Artmed: Porto Alegre, Brazil)

Ferreira CS, Piedade MTF, Tiné MAS, Rossato DR, Parolin P, Buckeridge MS (2009) The role of carbohydrates in seed germination and seedling establishment of Himatanthus sucuuba, an Amazonian tree with populations adapted to flooded and non-flooded conditions. Annals of Botany 104, 1111–1119.
The role of carbohydrates in seed germination and seedling establishment of Himatanthus sucuuba, an Amazonian tree with populations adapted to flooded and non-flooded conditions.CrossRef | 1:CAS:528:DC%2BC3cXjvVGlsg%3D%3D&md5=5a7bbd4189b0ac1d6a2d09748b279eaaCAS | open url image1

Figueiredo MA, Baêta HE, Kozovits AR (2012) Germination of native grasses with potential application in the recovery of degraded areas in Quadrilátero Ferrífero, Brazil. Biota Neotropica 12, 118–123.
Germination of native grasses with potential application in the recovery of degraded areas in Quadrilátero Ferrífero, Brazil.CrossRef | open url image1

Garcia IS, Souza A, Barbedo CJ, Dietrich SMC, Figueiredo-Ribeiro RCL (2006) Changes in soluble carbohydrates during storage of seeds of Caesalpina echinata Lam. (Brazilwood), an endangered leguminous tree from Brazilian Atlantic Forest. Brazilian Journal of Biology 66, 739–745.
Changes in soluble carbohydrates during storage of seeds of Caesalpina echinata Lam. (Brazilwood), an endangered leguminous tree from Brazilian Atlantic Forest.CrossRef | 1:STN:280:DC%2BD28vot1eqsA%3D%3D&md5=522d4162c92333a90cd51063e7beed92CAS | open url image1

Guimarães CC Faria JMR Oliveira JM Silva EAA 2011 Avaliação da perda da tolerância à dessecação e da quantidade de DNA nuclear em sementes de Peltophorum dubium (Spreng.) Taubert durante e após a germinação. Revista Brasileira de Sementes 33 207 215 dx.doi.org/10.1590/S0101-31222011000200002

Juliano BO, Varner JE (1969) Enzymic degradation of starch granules in the cotyledons of germinating peas. Plant Physiology 44, 886–892.
Enzymic degradation of starch granules in the cotyledons of germinating peas.CrossRef | 1:CAS:528:DyaF1MXksFOltbs%3D&md5=ffbd1170916ac5c95cb216c2845bb53bCAS | 5799049PubMed | open url image1

Leduc SNM, Silva JPN, Gaspar M, Barbedo CJ, Figueiredo-Ribeiro RCL (2012) Non-structural carbohydrates of immature seeds of Caesalpinia echinata (Leguminosae) are involved in the induction of desiccation tolerance. Australian Journal of Botany 60, 42–48.
Non-structural carbohydrates of immature seeds of Caesalpinia echinata (Leguminosae) are involved in the induction of desiccation tolerance.CrossRef | 1:CAS:528:DC%2BC38XivVyiu7s%3D&md5=eea5d12ebc9be878dff3305512efa8f1CAS | open url image1

Lorenzi H (2008) ‘Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil.’ (Editora Plantarum: Nova Odessa, Brazil)

Malavasi CM, Klein J, Malavasi MM (2010) Efeito de um protetor físico na semeadura direta de duas espécies florestais em área de domínio ciliar. Revista Árvore 34, 781–787.
Efeito de um protetor físico na semeadura direta de duas espécies florestais em área de domínio ciliar.CrossRef | open url image1

Mattei VL, Rosenthal MD (2002) Semeadura direta de canafístula (Peltophorum dubium (Spreng.) Taub.) na regeneração de capoeiras. Revista Árvore 26, 649–654.
Semeadura direta de canafístula (Peltophorum dubium (Spreng.) Taub.) na regeneração de capoeiras.CrossRef | open url image1

Max JCM, Melo ACG, Faria HH (2004) Comportamento de seis espécies nativas de dois grupos ecológicos plantadas em diferentes espaçamentos em reflorestamento ciliar (cap 23). In ‘Pesquisa em conservação e recuperação ambiental no oeste paulista’. (Eds O Vilas Boas, G Durigan) pp. 385–395. (Instituto Florestal/Secretaria do Meio Ambiente: São Paulo, Brazil)

Mello JIO, Barbedo CJ, Salatino A, Figueiredo-Ribeiro RCL (2010) Reserve carbohydrates and lipids from the seeds of four tropical tree species with different sensitivity to desiccation. Brazilian Archives of Biology and Technology 53, 889–899.
Reserve carbohydrates and lipids from the seeds of four tropical tree species with different sensitivity to desiccation.CrossRef | 1:CAS:528:DC%2BC3cXhtlWjsbvF&md5=9bc504723236489d8c0609594ad48ad2CAS | open url image1

Mello JIO, Centeno DC, Barbedo CJ, Figueiredo-Ribeiro RCL (2011) Changes in carbohydrate composition in seeds of three tropical tree species stored at freezing temperature. Seed Science and Technology 39, 465–480.

Meneguello GE, Mattei VL (2004) Semeadura direta de timbaúva (Enterolobium contortisiliquum), canafístula (Peltophorum dubium) e cedro (Cedrela fissilis) em campos abandonados. Ciência florestal 14, 21–27.

Obendorf RL, Górecki RJ (2012) Soluble carbohydrates in legume seeds. Seed Science Research 22, 219–242.
Soluble carbohydrates in legume seeds.CrossRef | 1:CAS:528:DC%2BC38Xhs12mu73O&md5=90c241a95bef3f5c4b36cbebe2368aa8CAS | open url image1

Perez SCJG, Fanti SC, Casali CA (1999) Influência do armazenamento, substrato, envelhecimento precoce e profundidade de semeadura na germinação de canafístula. Bragantia 58, 57–68.
Influência do armazenamento, substrato, envelhecimento precoce e profundidade de semeadura na germinação de canafístula.CrossRef | open url image1

Potomati A, Buckeridge MS (2002) Effect of abcisic acid on the mobilization of galactomannan and embryo development of Sesbania virgata (Cav). Pers (Leguminosae – Faboideae). Revista Brasileira de Botânica 25, 303–310.
Effect of abcisic acid on the mobilization of galactomannan and embryo development of Sesbania virgata (Cav). Pers (Leguminosae – Faboideae).CrossRef | 1:CAS:528:DC%2BD3sXisVCmtLk%3D&md5=6fefad5c96df5ae9344942ef3c675e6aCAS | open url image1

Prajapati VD, Jani GK, Moradiya NG, Randeria NP, Nagar BJ, Nikhil NN, Variya BC (2013) Galactomannan: a versatile biodegradable seed polysaccharide. International Journal of Biological Macromolecules 60, 83–92.
Galactomannan: a versatile biodegradable seed polysaccharide.CrossRef | 1:CAS:528:DC%2BC3sXhtFyjtrzP&md5=74cf87323d45439a8e3d7af806457d89CAS | 23707734PubMed | open url image1

Pritchard SL, Charlton WL, Baker A, Graham IA (2002) Germination and storage reserve mobilization are regulated independently in Arabidopsis. The Plant Journal 31, 639–647.
Germination and storage reserve mobilization are regulated independently in Arabidopsis.CrossRef | 1:CAS:528:DC%2BD38Xnsl2hurg%3D&md5=8ebb3dceb67d8c73dad335efddf90602CAS | 12207653PubMed | open url image1

Reid GJS, Bewley D (1979) A dual role for the endosperm and its galactomannan reserves in the germinative physiology of Fenugreek (Trigoneila foenum-graecum L.), an endospermic leguminous seed. Planta 147, 145–150.
A dual role for the endosperm and its galactomannan reserves in the germinative physiology of Fenugreek (Trigoneila foenum-graecum L.), an endospermic leguminous seed.CrossRef | 1:CAS:528:DyaL3cXlsFahtw%3D%3D&md5=93e05c6c64a4fe61e8be59ed4e37077cCAS | open url image1

Roberts EH (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499–514.

Rodríguez-Gacio MC, Iglesias-Fernández R, Carbonero P, Matilla AJ (2012) Softening-up mannan-rich cell walls. Journal of Experimental Botany 63, 3976–3988.
Softening-up mannan-rich cell walls.CrossRef | open url image1

Sánchez-Linares L, Gavilanes-Ruíz M, Díaz-Pontones D, Guzmán-Chávez F, Calzada-Alejo V, Zurita-Villegas V, Luna-Loaiza V, Moreno-Sánchez R, Bernal-Lugo I, Sánchez-Nieto S (2012) Early mobilization and radicle protrusion in maize germination. Journal of Experimental Botany 63, 4513–4526.
Early mobilization and radicle protrusion in maize germination.CrossRef | 22611232PubMed | open url image1

Santana DG, Ranal MA (2004) ‘Análise da germinação: um enfoque estatístico.’ (Universidade de Brasília: Brasília)

Santos Junior NA, Botelho SA, Davide AC (2004) Estudo da germinação e sobrevivência de espécies arbóreas em sistema de semeaduradireta, visando à recomposição de mata ciliar. Cerne 10, 103–117.

Scalon SPQ, Mussury RM, Wathier F, Gomes AA, Silva KA, Pierezan L, Scalon Filho H (2005) Armazenamento, germinação de sementes e crescimento inicial de mudas de Enterolobium contortisiliquum (Vell.) Morong. Acta Scientiarum Biological Sciences 27, 107–112.
Armazenamento, germinação de sementes e crescimento inicial de mudas de Enterolobium contortisiliquum (Vell.) Morong.CrossRef | open url image1

Soares PG, Rodrigues RR (2008) Semeadura direta de leguminosas florestais: efeito da inoculação com rizóbio na emergência de plântulas e crescimento inicial no campo. Scientia Forestalis 36, 115–121.

Somogyi M (1945) A new reagent for the determination of sugars. The Journal of Biological Chemistry 160, 61–68.

Tonini PP, Lisboa CGS, Silva CO, Mazzoni-Viveiros SC, Buckeridge MS (2006) Testa is involved in the control of storage mobilization in seeds of Sesbania virgata (Cav.) Pers., a tropical legume tree from of the Atlantic Forest. Trees 21, 13–21.
Testa is involved in the control of storage mobilization in seeds of Sesbania virgata (Cav.) Pers., a tropical legume tree from of the Atlantic Forest.CrossRef | open url image1

Tonini PP, Carrara TB, Buckeridge MS (2010) Storage proteins and cell wall mobilisation in seeds of Sesbania virgata (Cav.) Pers. (Leguminosae). Trees 24, 675–684.
Storage proteins and cell wall mobilisation in seeds of Sesbania virgata (Cav.) Pers. (Leguminosae).CrossRef | 1:CAS:528:DC%2BC3cXoslylt78%3D&md5=2b781f99f9b3c8d6498603fc008335deCAS | open url image1

Vilas Boas O, Max JCM, Nakata H (2004) Recuperação da cobertura vegetal – Crescimento e sobrevivência das mudas de essências nativas produzidas em diferentes recipientes (cap 16). In ‘Pesquisa em conservação e recuperação ambiental no oeste paulista’. (Eds O Vilas Boas, G Durigan) pp. 293–304. (Instituto Florestal/Secretaria do Meio Ambiente: São Paulo, Brazil)

Wanli Z, Leihong L, Perez SCJGA (2001) Pré-condicionamento e seus efeitos em sementes de canafístula (Peltophorum dubium (Spreng.) Taub.). Revista Brasileira de Sementes 23, 146–153.

Wielewick AP, Leonhoardt C, Schlindwein G, Medeiros ACS (2006) Proposta de padrões de germinação e teor de água para sementes de algumas espécies florestais presentes na resgião sul do Brasil. Revista Brasileira de Sementes 28, 191–197.
Proposta de padrões de germinação e teor de água para sementes de algumas espécies florestais presentes na resgião sul do Brasil.CrossRef | open url image1



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