In vitro Propagation of Nothofagus obliqua (Fagaceae).

Abstract

Suitable culture conditions were investigated for in vitro propagation through shoot culture of seedlings and the acclimatization of the rooted plantlets of Nothofagus obliqua. Explant size and leaf expansion were critical for culture initiation. A medium with a low salt concentration and the addition of L-glutamine, as broadleaved tree medium, was suitable for shoot multiplication and it was influenced by both cytokinin and auxin. A multiplication rate of x5 has been obtained with 0.55 micro M benzyladenine (BA), and 0.09 micro M BA and 0.09 micro M IBA at day 63. Rooted shoots (51.9%) were obtained with 0.61 IBA and 1 week darkness at the induction stage. Rooted plants were then successfully acclimatised and 75% survival rate was obtained after 4 months.