Relict islands of the temperate rainforest tree Aextoxicon punctatum (Aextoxicaceae) in semi-arid Chile: genetic diversity and biogeographic history
Mariela C. Núñez-Ávila A B C D and Juan J. Armesto A CA CMEB, Laboratorio de Ecología Forestal, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
B Present address: Instituto de Silvicultura, Universidad Austral de Chile, Valdivia, Chile.
C CASEB, Departamento de Ecología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Casilla 114-D, Santiago, Chile.
D Corresponding author. Email: marielanunez@uach.cl
Australian Journal of Botany 54(8) 733-743 https://doi.org/10.1071/BT06022
Submitted: 8 February 2006 Accepted: 10 July 2006 Published: 29 November 2006
Abstract
Aextoxicon punctatum, the only representative of the family Aextoxicaceae, is a tree species endemic to temperate forests of western South America. This species exhibits a disjunct distribution pattern, with few isolated populations occurring on coastal hilltops of the Chilean semi-arid zone (SAZ), 30–32°S; small populations mixed with sclerophyllous forest in some gorges of the central Chilean Mediterranean coastal range (MCR), 32–39°S; more continuous forests extended along the southern temperate coastal range (TCR), 39–43°S; and fragmented populations eastward in the south temperate central depression and Andean foothills (TAF), 39–41°S. This geographic disjunction is the result of climatic and tectonic changes that took place in southern South America since the late Tertiary, leading to the isolation of forest fragments in the SAZ from the rest of the distribution. According to palynological evidence, populations of TCR and TAF originated from postglacial population expansions from refuges located on the coastal range, north of 40°S. We examined how the present genetic structure and diversity of Aextoxicon populations in Chile reflects this biogeographic history. Random amplified polymorphic DNA (RAPD) markers were used to characterise genetic differences within and among 16 populations of this species throughout its natural range. AMOVA and UPGMA analysis showed high genetic differentiation between the geographically closer SAZ and MCR populations, suggesting a long history of restricted genetic exchange between populations in these two zones. Estimates of Shannon’s genetic diversity and percentage polymorphism were relatively low compared with other southern temperate forest trees (mainly conifers) that were less affected by the glaciations. Positive correlations between genetic and geographic distances were found for TCR but not for TAF populations, suggesting earlier postglacial population expansion southwards along the TCR and more recent eastward migration from coastal locations to TAF during the Holocene.
Acknowledgments
We dedicate this paper to our colleague Carolina Villagrán, who introduced us to the enigmatic history of Olivillo forests and stimulated us to pursue this study. We thank many friends and colleagues for helping with field sampling. Pete Hollingsworth, Adrian Newton, Richard Ennos, Sylvain Fulgeiron, Mark Hershkovitz and Elie Poulin offered technical advice and comments on earlier versions of this manuscript. CONAF and landowners are thanked for allowing access to research sites. This work was supported by graduate fellowships from CMEB (P99-103F-ICM) and from Universidad de Chile (PG/28/02), and by grants from FONDAP-FONDECYT 1501-0001 (CASEB, Pontificia Universidad Católica de Chile) and BIOCORES Project funded by EC under INCO IV program (contract ICA 4-CT-2001-10095). This is a contribution to the research program of Senda Darwin Biological Station, Chile.
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