Register      Login
Healthcare Infection Healthcare Infection Society
Official Journal of the Australasian College for Infection Prevention and Control
RESEARCH ARTICLE

Measuring of ATP bioluminescence as a means of assessing washer disinfector performance and potentially as a means of validating the decontamination process

Raymond Heathcote A C and Brett Stadelmann B
+ Author Affiliations
- Author Affiliations

A Epworth Freemasons Hospital, Clarendon Street, East Melbourne, Vic. 3002, Australia.

B Central Sterile Supply Department, Epworth Freemasons Hospital, Clarendon Street, East Melbourne, Vic. 3002, Australia.

C Corresponding author. Email: raymond.heathcote@epworth.org.au

Healthcare Infection 14(4) 147-151 https://doi.org/10.1071/HI09019
Published: 21 December 2009

Abstract

This paper reports on the results of using a commercially available adenosine tri-phosphate bioluminescence (ATP) hand-held device for assessing the level of contamination on surgical instruments throughout the decontamination process. The report demonstrates that this technique may be a reliable point-of-use method for quantitatively assessing washer disinfector function and possibly a means of performing a washer disinfector validation process.


References


[1] Gardner J , Peel M . Sterilization, Disinfection and Infection Control, Third Edition, Marrickville, Australia, Churchill Livingstone (1998) Page 3.

[2] Hygiena Systemsure II product information. Downloaded from website http://www.hygienausa.com/systemsure.html on 05/06/2009.

[3] Obee P,  Griffith C,  Cooper R,  Cooke R,  Bennion N,  Lewis M. Real time monitoring in managing the decontamination of flexible gastrointestinal endoscopes. Am J Infect Control 2005; 33 202–6.
Crossref | GoogleScholarGoogle Scholar | PubMed |

[4] Hansen D,  Hilgenhöner M,  Popp W. ATP bioluminescence – for kitchen hygiene and cleaning control of surgical instruments. Intern J Infect Control 2008; 4 1–4.
Crossref | GoogleScholarGoogle Scholar |

[5] Murdoch H,  Taylor D,  Dickinson J,  Walker JT,  Perrett D,  Raven NDH, et al. Surface decontamination of surgical instruments: an ongoing dilemma. J Hosp Infect 2006; 63 432–8.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed |

[6] AS/NZS4187:2003 Australian/New Zealand Standard™ Cleaning, disinfecting and sterilizing reusable medical and surgical instruments and equipment, and maintenance of associated environments in health care facilities. Section 2.9.1, Page 21, Section 2.9.2.4 (j), Page 23, Section 2.11, Page 27.

[7] Jimenez L. Adenosine triphosphate bioluminescence analysis for rapid screening of microbial contamination in non-sterile pharmaceutical samples. PDA J Pharm Sci Technol 2004; 58(3): 159–68.
CAS | PubMed |

[8] Aycicek H,  Oguz U,  Karci K. Comparison of results of ATP bioluminescence and traditional hygiene swabbing methods for the determination of surface cleanliness at a hospital kitchen. Int J Hyg Environ Health 2006; 209(2): 203–6.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed |

[9] Lewis T,  Griffith C,  Gallo M,  Weinbren M. A modified ATP benchmark for evaluating the cleaning of some hospital environmental surfaces. J Hosp Infect 2008; 69 156–63.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed |

[10] Andersen BM,  Rasch M,  Kvist J,  Tollefsen T,  Lukkassen R,  Sandvik L, et al. Floor cleaning: effect on bacteria and organic materials in hospital rooms. J Hosp Infect 2009; 71(1): 57–65.
Crossref | GoogleScholarGoogle Scholar | CAS | PubMed |

[11] International Standard Organization. ISO 15883–1 First edition 2006–04–15 Washer-disinfectors – Part 1: General requirements, terms and definitions and tests in section, Annex C.