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Protocols in ecological and environmental plant physiology


Article << Previous     |     Next >>   Contents Vol 61(8)

Managing nutrient regimes improves seedling root-growth potential of framework banksia-woodland species

Erin Griffiths A B and Jason C. Stevens A B C

A Science Directorate, Kings Park and Botanic Garden, West Perth, WA 6005, Australia.
B School of Plant Biology, University of Western Australia, Nedlands, WA 6009, Australia.
C Corresponding author. Email: jason.stevens@bgpa.wa.gov.au

Australian Journal of Botany 61(8) 600-610 http://dx.doi.org/10.1071/BT13181
Submitted: 12 July 2013  Accepted: 18 December 2013   Published: 21 March 2014

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Limited success of restoring framework banksia-woodland species has been attributed to the failure of seedlings to establish deep root systems before the onset of the summer drought. The present glasshouse study investigated how optimising nutrient application during nursery production may increase new-root production after outplanting. Two experimental streams were established to (1) optimise nutrient application rates during nursery production and (2) utilise nutrient-loading techniques to improve root production of Banksia menziesii R.Br., Banksia attenuata R.Br. and Eucalyptus todtiana F.Muell after outplanting. Optimal nutrient-application rates were determined by measuring plant growth and internal nutrient responses to eight application levels of slow-release fertiliser (0–18 kg m–3, nitrogen (N) : phosphorus (P) : potassium (K) = 17 : 1.6 : 8.7). Nutrient-loading treatments utilised seedlings that had been grown under common industry fertiliser conditions (3 kg m–3 native Osmocote, N : P : K = 17 : 1.6 : 8.7) supplied with ‘low’ or ‘high’ loading doses of liquid Thrive continuously over 6 weeks, immediately before outplanting. Seedlings from both experiments were then outplanted to 1-m-deep poly-pipe tubes containing habitat soil. After 12 weeks, plants were harvested and new-root production and shoot growth were measured. Optimal concentrations of slow-release fertiliser for maximum outplanting success as indicated by increased root investment (root : shoot ratio and new-root production) were 8–12 kg m–3 for all species. Nutrient loading increased N and P concentrations of plants by up to 80% and 127%, respectively, by luxury nutrient consumption, and after planting, nutrient-loaded seedlings produced 1.5-fold the biomass of conventionally fertilised seedlings, this being the result of greater root productivity. In conclusion, optimising nursery nutrient regimes for framework species may increase root-growth potential, assisting in improving plant establishment in restoration programs.

Additional keywords: fertiliser, nursery production, nutrient loading, restoration.


Beard JS (1989) Definition and location of the banksia woodlands. Journal of the Royal Society of Western Australia 71, 85–86.

Benigno S, Dixon K, Stevens J (2012) Increasing soil water retention with native-sourced mulch improves seedling establishment in postmine Mediterranean sandy soils. Restoration Ecology
CrossRef |

Boivin JR, Miller BD, Timmer VR (2002) Late-season fertilisation of Picea mariana seedlings under greenhouse culture: biomass and nutrient dynamics. Annals of Forest Science 59, 255–264.
CrossRef |

Close DC, Bail I, Hunter S, Beadle CL (2005) Effects of exponential nutrient-loading on morphological and nitrogen characteristics and on after-planting performance of Eucalyptus globulus seedlings. Forest Ecology and Management 205, 397–403.
CrossRef |

Cromer RN, Jarvis PG (1990) Growth and biomass partitioning in Eucalyptus grandis seedlings in response to nitrogen supply. Australian Journal of Plant Physiology 17, 503–515.
CrossRef |

Davis AS, Jacobs DF (2005) Quantifying root systems quality of nursery seedlings and relationship to outplanting performance. New Forests 30, 295–311.
CrossRef |

Dawson TE, Pate JS (1995) Seasonal water uptake and movement in root systems of Australian phreatophytic plants of dimorphic root morphology: a stable isotope investigation. Oecologia 107, 1432–1439.

Dodd J, Griffin EA (1989) Floristics of the banksia woodland. Journal of the Royal Society of Western Australia 18, 281–293.

Enright NJ, Lamont BB (1992) Survival, growth and water relations of banksia seedlings on a sand mine rehabilitation site and adjacent scrub–heath sites. Journal of Applied Ecology 29, 663–671.
CrossRef |

Fox BJ, Fox MD, Taylor JE, Jackson GP, Simpson J, Higgs P, Rebec L, Avery R (1996) Comparison of regeneration following burning, clearing or mineral sand mining at Tomago, NSW: structure and growth of vegetation. Australian Journal of Ecology 21, 184–199.

Genstat (2003) ‘GenStat® release 7.1.’ (VSN International: Oxford, UK)

Groom PK (2002) Seedling water stress response of two sandplain banksia species differing in ability to tolerate drought. Journal of Mediterranean Ecology 3, 3–9.

Grose PJ (2013) Growth of slender banksia from seedling planting, with and without fertiliser. Ecological Management & Restoration 14, 144–147.
CrossRef |

Handreck KA (1991) Interactions between iron and phosphorus in the nutrition of Banksia ericifolia L.f. var. ericifolia (Proteaceae): in soil-less potting media. Australian Journal of Botany 39, 373–384.
CrossRef |

Hopper SD, Burbidge AH (1989) Conservation status of Banksia woodlands on the Swan Coastal Plain. Journal of the Royal Society of Western Australia 71, 85–86.

Ingestad T, Agren GI (1988) Nutrient uptake and allocation at steady-state nutrition. Plant Physiology 72, 450–459.
CrossRef |

Ingestad T, Lund AB (1986) Theory and techniques for steady state mineral nutrition and growth of plants. Scandinavian Journal of Forest Research 1, 439–453.
CrossRef |

Isaac RA, Johnson WC (1976) Determination of total nitrogen in plant tissue, using a block digester. Journal - Association of Official Analytical Chemists 59, 98–100.

Jeschke WD, Pate JS (1995) Mineral nutrition and transport in xylem and phloem of Banksia Prionotes (Proteaceae): a tree with dimorphic root morphology. Journal of Experimental Botany 46, 895–905.
CrossRef |

Keighery K (2011) Banksia woodlands: a Perth Icon. In ‘Perth’s banksia woodlands, precious and under threat: proceedings of a symposium on the ecology of these ancient woodlands and their need for protection from neglect and destruction, 25 March 2011’. (Ed. K Sarti) pp. 3–11. (Urban Bushland Council: WA)

Kirschbaum MUF, Bellingham DW, Cromer RN (1992) Growth analysis of the effect of phosphorus nutrition on seedling of Eucalyptus grandis. Australian Journal of Plant Physiology 19, 55–66.
CrossRef |

Kuo J, Hocking PJ, Pate JS (1982) Nutrient reserves in seeds of selected proteaceous species from south-western Australia. Australian Journal of Botany 30, 231–249.
CrossRef |

McQuaker NR, Brown DF, Kluckner PD (1979) Digestion of environmental materials for analysis by inductively coupled plasma–atomic emission spectrometry. Analytical Chemistry 51, 1082–1084.
CrossRef |

Milberg P, Lamont BB (1997) Seed/cotyledon size and nutrient content play a major role in early performance of species on nutrient-poor soils. New Phytologist 137, 665–672.
CrossRef |

Milberg P, Perez-Fernandez MA, Lamont BB (1998) Seedling growth response of added nutrients depends on seed size in three woody genera. Journal of Ecology 86, 624–632.
CrossRef |

Parks SE, Haigh AM, Harris AM (2007) The responses of six species of Proteaceae, in containers, to controlled-release fertiliser. Communications in Soil Science and Plant Analysis 38, 2227–2237.
CrossRef |

Pate JS, Jeschke DW, Dawson TE, Raphael C, Hartung W (1998) Growth and seasonal utilisation of water and nutrients by Banksia prionotes. Australian Journal of Botany 46, 511–532.
CrossRef |

Quoreshi M, Timmer VR (2000) Early outplanting performance of nutrient-loaded containerised black spruce seedlings inoculated with Laccaria bicolor: a bioassay study. Canadian Journal of Forest Research 30, 744–752.
CrossRef |

Rokich DP, Dixon KW (2007) Recent advances in restoration ecology, with a focus on the banksia woodland and the smoke germination tool. Australian Journal of Botany 55, 375–389.
CrossRef |

Rokich DP, Meney KA, Dixon KW, Sivasithamparam K (2001) The impact of soil disturbance on root development in woodland communities in Western Australia. Australian Journal of Botany 49, 169–183.
CrossRef |

Salifu KF, Timmer VR (2003) Optimizing nitrogen loading of Picea mariana seedlings during nursery culture. Canadian Journal of Forest Research 33, 1287–1294.
CrossRef |

Sheriff DW, Nambiar EKS (1991) Nitrogen nutrition, growth and gas exchange in Eucalyptus globulus Labill. seedlings. Australian Journal of Plant Physiology 18, 37–52.
CrossRef |

Stock WD, Pate JS, Delfs J (1990) Influence of seed size and quality on seedling development under low nutrient conditions in five Australian and South African members of Proteaceae. Journal of Ecology 78, 1005–1020.
CrossRef |

Timmer VR (1996) Exponential nutrient loading: a new fertilisation technique to improve seedling performance on competitive sites. New Forests 13, 275–295.

Timmer VR, Munson AD (1991) Site-specific growth and nutrition of planted Picea mariana in the Ontario Clay Belt: nutrient loading response. IV. Nutrient loading response. Canadian Journal of Forest Research 21, 1058–1065.
CrossRef |

Weller R (2009) ‘Boomtown 2050: scenarios for a rapidly growing city.’ (UWA Publishing: Perth)

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