Investigating the effect of larval instar, post-harvest treatments, and substrate on the nutritional profile of black soldier fly larvae (Hermetia illucens)
Shanmugam Alagappan A B , Hung Hong C , Deirdre Mikkelsen A C , Sandra Olarte Mantilla A B , Peter James


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Handling Editor: Konstantinos Mountzouris
Abstract
Black soldier fly larvae (BSFL) is considered to be a sustainable animal feed ingredient, that feeds in different organic waste streams. It is well established that the nutritional profile of BSFL is influenced by the substrate used. However, the impact on the overall nutritional quality of the larvae when reared on food waste streams in an industrial setting is unknown.
The objective of this study is to determine the nutritional quality and chemical composition of BSFL from two instars (fifth and sixth) reared on five different food waste streams from two different waste facilities and post-harvest treatments.
BSFL were reared with five different waste streams, viz. soy waste (S), customised bread–vegetable waste (B), supermarket waste (WL), childcare centre waste (CC) and a food waste mixture (WB); two larvae instars (fifth and sixth) and post-harvest treatments (blanching and drying) were analysed for proximate composition, and amino, fatty acid and mineral contents.
The blanching and drying treatments did not affect the chemical composition of the larvae; however, they had a negative effect on fatty acid content and reduced the concentration of certain amino acids. The mineral content of BSFL was increased by drying, whereas blanching did not result in leaching except for sodium. The larval instar played a role in influencing the crude fat and crude protein content, with a higher fatty acid content in sixth instar BSFL. Mineral and amino acid profiles were found to be influenced by both the larval instar and the food waste stream used for rearing.
This study showed the interdependent relationship between substrate, instars, and the post-harvest treatments in influencing the nutritional properties of the larvae.
The outcomes of this study will assist manufacturers in substrate selection and enable them to custom tailor their operations to arrive at a product with desired nutritional properties.
Keywords: alternative proteins, black soldier fly larvae, circular economy, composition, food waste streams, nutritional quality, post-harvest treatments.
References
Alagappan S, Hoffman LC, Mantilla SMO, Mikkelsen D, James P, Yarger O, Cozzolino D (2022a) Near Infrared spectroscopy as a traceability tool to monitor black soldier fly larvae (Hermetia illucens) intended as animal feed. Applied Sciences 12(16), 8168.
| Crossref | Google Scholar |
Alagappan S, Rowland D, Barwell R, Cozzolino D, Mikkelsen D, Olarte Mantilla SM, James P, Yarger O, Hoffman L (2022b) Organic side streams (bioproducts) as substrate for black soldier fly (Hermetia illucens) intended as animal feed: chemical safety issues. Animal Production Science 62(17), 1639-1651.
| Crossref | Google Scholar |
Alagappan S, Dong A, Mikkelsen D, Hoffman LC, Mantilla SMO, James P, Yarger O, Cozzolino D (2023) Near infrared spectroscopy for prediction of yeast and mould counts in black soldier fly larvae, feed and frass: a proof of concept. Sensors 23(15), 6946.
| Crossref | Google Scholar |
Amicarelli V, Lagioia G, Bux C (2021) Global warming potential of food waste through the life cycle assessment: an analytical review. Environmental Impact Assessment Review 91, 106677.
| Crossref | Google Scholar |
Barbi S, Macavei LI, Fuso A, Luparelli AV, Caligiani A, Ferrari AM, Maistrello L, Montorsi M (2020) Valorization of seasonal agri-food leftovers through insects. Science of The Total Environment 709, 136209.
| Crossref | Google Scholar |
Barragán-Fonseca K, Pineda-Mejia J, Dicke M, Van Loon JJ (2018) Performance of the black soldier fly (Diptera: Stratiomyidae) on vegetable residue-based diets formulated based on protein and carbohydrate contents. Journal of Economic Entomology 111(6), 2676-2683.
| Crossref | Google Scholar |
Barragan-Fonseca KB, Gort G, Dicke M, van Loon JJA (2019) Effects of dietary protein and carbohydrate on life-history traits and body protein and fat contents of the black soldier fly Hermetia illucens. Physiological Entomology 44(2), 148-159.
| Crossref | Google Scholar |
Bessa LW, Pieterse E, Marais J, Hoffman LC (2020) Why for feed and not for human consumption? The black soldier fly larvae. Comprehensive Reviews in Food Science and Food Safety 19(5), 2747-2763.
| Crossref | Google Scholar |
Beyers M, Coudron C, Ravi R, Meers E, Bruun S (2023) Black soldier fly larvae as an alternative feed source and agro-waste disposal route – a life cycle perspective. Resources, Conservation and Recycling 192, 106917.
| Crossref | Google Scholar |
Cammack JA, Tomberlin JK (2017) The Impact of diet protein and carbohydrate on select life-history traits of the black soldier fly Hermetia illucens (L.) (Diptera: Stratiomyidae). Insects 8(2), 56.
| Crossref | Google Scholar |
Cheng M, McCarl B, Fei C (2022) Climate change and livestock production: a literature review. Atmosphere 13(1), 140.
| Crossref | Google Scholar |
Chua ET, Brunner M, Atkin R, Eltanahy E, Thomas-Hall SR, Schenk PM (2019) The ionic liquid cholinium arginate is an efficient solvent for extracting high-value Nannochloropsis sp. lipids. ACS Sustainable Chemistry & Engineering 7(2), 2538-2544.
| Crossref | Google Scholar |
Danieli PP, Lussiana C, Gasco L, Amici A, Ronchi B (2019) The effects of diet formulation on the yield, proximate composition, and fatty acid profile of the black soldier fly (Hermetia illucens L.) prepupae intended for animal feed. Animals 9(4), 178.
| Crossref | Google Scholar |
Do S, Koutsos EA, Utterback PL, Parsons CM, de Godoy MRC, Swanson KS (2021) Amino acid digestibility and digestible indispensable amino acid score-like values of black soldier fly larvae fed different forms and concentrations of calcium using the precision-fed cecectomized rooster assay. Journal of Animal Science 99(6), skab124.
| Crossref | Google Scholar |
Dolan SP, Capar SG (2002) Multi-Element analysis of food by microwave digestion and inductively coupled plasma-atomic emission spectrometry. Journal of Food Composition and Analysis 15(5), 593-615.
| Crossref | Google Scholar |
Ebeneezar S, Linga Prabu D, Teipal CS, Jeena NS, Summaya R, Chandrasekar S, Sayooj P, Vijayagopa P (2021) Nutritional evaluation, bioconversion performance and phylogenetic assessment of black soldier fly (Hermetia illucens, Linn. 1758) larvae valorized from food waste. Environmental Technology & Innovation 23, 101783.
| Crossref | Google Scholar |
Eggink KM, Donoso IG, Dalsgaard J (2023) Optimal dietary protein to carbohydrate ratio for black soldier fly (Hermetia illucens) larvae. Journal of Insects as Food and Feed 9(6), 789-798.
| Crossref | Google Scholar |
Erhard AL, Águas Silva M, Damsbo-Svendsen M, Menadeva Karpantschof B-E, Sørensen H, Bom Frøst M (2023) Acceptance of insect foods among Danish children: effects of information provision, food neophobia, disgust sensitivity, and species on willingness to try. Food Quality and Preference 104, 104713.
| Crossref | Google Scholar |
Ewald N, Vidakovic A, Langeland M, Kiessling A, Sampels S, Lalander C (2020) Fatty acid composition of black soldier fly larvae (Hermetia illucens) – Possibilities and limitations for modification through diet. Waste Management 102, 40-47.
| Crossref | Google Scholar |
Ferrari L, Cattaneo DMIR, Abbate R, Manoni M, Ottoboni M, Luciano A, von Holst C, Pinotti L (2023) Advances in selenium supplementation: from selenium-enriched yeast to potential selenium-enriched insects, and selenium nanoparticles. Animal Nutrition 14, 193-203.
| Crossref | Google Scholar |
Fischer H, Romano N (2021) Fruit, vegetable, and starch mixtures on the nutritional quality of black soldier fly (Hermetia illucens) larvae and resulting frass. Journal of Insects as Food and Feed 7(3), 319-328.
| Crossref | Google Scholar |
Fitriana EL, Laconi EB, Astuti DA, Jayanegara A (2022) Effects of various organic substrates on growth performance and nutrient composition of black soldier fly larvae: a meta-analysis. Bioresource Technology Reports 18, 101061.
| Crossref | Google Scholar |
Gao Z, Wang W, Lu X, Zhu F, Liu W, Wang X, Lei C (2019) Bioconversion performance and life table of black soldier fly (Hermetia illucens) on fermented maize straw. Journal of Cleaner Production 230, 974-980.
| Crossref | Google Scholar |
Gold M, Tomberlin JK, Diener S, Zurbrügg C, Mathys A (2018) Decomposition of biowaste macronutrients, microbes, and chemicals in black soldier fly larval treatment: a review. Waste Management 82, 302-318.
| Crossref | Google Scholar |
Gold M, Cassar CM, Zurbrügg C, Kreuzer M, Boulos S, Diener S, Mathys A (2020) Biowaste treatment with black soldier fly larvae: increasing performance through the formulation of biowastes based on protein and carbohydrates. Waste Management 102, 319-329.
| Crossref | Google Scholar |
Hashmi MS, Akhtar S, Ismail T (2021) Biochemical characterization of vegetables wastes and development of functional bread: Vegetables wastes based functional bread. Journal of Microbiology, Biotechnology and Food Sciences 10(4), 691-696.
| Crossref | Google Scholar |
Heuel M, Kreuzer M, Sandrock C, Leiber F, Mathys A, Gold M, Zurbrügg C, Gangnat IDM, Terranova M (2021) Transfer of lauric and myristic acid from black soldier fly larval lipids to egg yolk lipids of hens is low. Lipids 56, 423-435.
| Crossref | Google Scholar | PubMed |
Hossain MS, Fawole FJ, Labh SN, Small BC, Overturf K, Kumar V (2021) Insect meal inclusion as a novel feed ingredient in soy-based diets improves performance of rainbow trout (Oncorhynchus mykiss). Aquaculture 544, 737096.
| Crossref | Google Scholar |
Isibika A, Simha P, Vinnerås B, Zurbrügg C, Kibazohi O, Lalander C (2023) Food industry waste – an opportunity for black soldier fly larvae protein production in Tanzania. Science of The Total Environment 858, 159985.
| Crossref | Google Scholar |
Kaczmarek A, Boguś M (2021) The metabolism and role of free fatty acids in key physiological processes in insects of medical, veterinary and forensic importance. PeerJ 9, e12563.
| Crossref | Google Scholar |
Kahsay BN, Moeller L, Imming P, Neubert RHH, Gebre-Mariam T (2022) Development and validation of a simple, selective, and accurate reversed-phase liquid chromatographic method with diode array detection (RP-HPLC/DAD) for the simultaneous analysis of 18 free amino acids in topical formulations. Chromatographia 85(7), 665-676.
| Crossref | Google Scholar |
Karkalas J (1985) An improved enzymic method for the determination of native and modified starch. Journal of the Science of Food and Agriculture 36(10), 1019-1027.
| Crossref | Google Scholar |
Kaya C, Generalovic TN, Ståhls G, Hauser M, Samayoa AC, Nunes-Silva CG, Roxburgh H, Wohlfahrt J, Ewusie EA, Kenis M, Hanboonsong Y, Orozco J, Carrejo N, Nakamura S, Gasco L, Rojo S, Tanga CM, Meier R, Rhode C, Picard CJ, Jiggins CD, Leiber F, Tomberlin JK, Hasselmann M, Blanckenhorn WU, Kapun M, Sandrock C (2021) Global population genetic structure and demographic trajectories of the black soldier fly, Hermetia illucens. BMC Biology 19(1), 94.
| Crossref | Google Scholar |
Lalander C, Diener S, Zurbrügg C, Vinnerås B (2019) Effects of feedstock on larval development and process efficiency in waste treatment with black soldier fly (Hermetia illucens). Journal of Cleaner Production 208, 211-219.
| Crossref | Google Scholar |
Lee JH, Kim T-K, Park S-Y, Kang M-C, Cha JY, Lim M-C, Choi Y-S (2023) Effects of blanching methods on nutritional properties and physicochemical characteristics of hot-air dried edible insect larvae. Food Science of Animal Resources 43(3), 428-440.
| Crossref | Google Scholar |
Li S, Zhu D, Li K, Yang Y, Lei Z, Zhang Z (2013) Soybean curd residue: composition, utilization, and related limiting factors. International Scholarly Research Notices 2013, 423590.
| Crossref | Google Scholar |
Liew CS, Yunus NM, Chidi BS, Lam MK, Goh PS, Mohamad M, Sin JC, Lam SM, Lim JW, Lam SS (2022) A review on recent disposal of hazardous sewage sludge via anaerobic digestion and novel composting. Journal of Hazardous Materials 423, 126995.
| Crossref | Google Scholar |
Liland NS, Biancarosa I, Araujo P, Biemans D, Bruckner CG, Waagbø R, Torstensen BE, Lock E-J (2017) Modulation of nutrient composition of black soldier fly (Hermetia illucens) larvae by feeding seaweed-enriched media. PLoS ONE 12(8), e0183188.
| Crossref | Google Scholar |
Liu X, Chen X, Wang H, Yang Q, ur Rehman K, Li W, Cai M, Li Q, Mazza L, Zhang J, Yu Z, Zheng L (2017) Dynamic changes of nutrient composition throughout the entire life cycle of black soldier fly. PLoS ONE 12(8), e0182601.
| Crossref | Google Scholar |
Liu Z, Minor M, Morel PCH, Najar-Rodriguez AJ (2018) Bioconversion of three organic wastes by black soldier fly (Diptera: Stratiomyidae) larvae. Environmental Entomology 47(6), 1609-1617.
| Crossref | Google Scholar |
Liu Z, de Souza TSP, Holland B, Dunshea F, Barrow C, Suleria HAR (2023) Valorization of food waste to produce value-added products based on its bioactive compounds. Processes 11(3), 840.
| Crossref | Google Scholar |
Liyanaarachchi GVV, Mahanama KRR, Somasiri HPPS, Punyasiri PAN, Kottawa-Arachchi JD (2020) Analysis of total amino acids in rice using a validated reversed-phase high performance liquid chromatographic method with diode array detection (RP-HPLC-DAD). International Journal of Analytical and Bioanalytical Methods 2, 008.
| Crossref | Google Scholar |
Lu S, Taethaisong N, Meethip W, Surakhunthod J, Sinpru B, Sroichak T, Archa P, Thongpea S, Paengkoum S, Purba RAP, Paengkoum P (2022) Nutritional composition of black soldier fly larvae (Hermetia illucens L.) and its potential uses as alternative protein sources in animal diets: a review. Insects 13(9), 831.
| Crossref | Google Scholar |
Makkar HPS, Tran G, Heuzé V, Ankers P (2014) State-of-the-art on use of insects as animal feed. Animal Feed Science and Technology 197, 1-33.
| Crossref | Google Scholar |
Maranga B, Kagali R, Mbogo K, Orina P, Munguti J, Ogello E (2022) Growth performance of african catfish (Clarias Gariepinus) fed on diets containing black soldier fly (Hermetia Illucens) larvae under aquaponic system. Aquaculture Studies 23(5), AQUAST910.
| Crossref | Google Scholar |
Meneguz M, Schiavone A, Gai F, Dama A, Lussiana C, Renna M, Gasco L (2018) Effect of rearing substrate on growth performance, waste reduction efficiency and chemical composition of black soldier fly (Hermetia illucens) larvae. Journal of the Science of Food and Agriculture 98(15), 5776-5784.
| Crossref | Google Scholar |
Montevecchi G, Macavei LI, Zanelli E, Benassi G, Pinotti G, D’Arco S, Buffagni S, Masino F, Maistrello L, Antonelli A (2023) Seasonal variability of the HO.RE.CA. food leftovers employed as a feeding substrate for black soldier fly (Hermetia illucens L.) larvae and effects on the rearing performance. Sustainable Chemistry and Pharmacy 33, 101061.
| Crossref | Google Scholar |
Nampijja Z, Kiggundu M, Kigozi A, Lugya A, Magala H, Ssepuuya G, Nakimbugwe D, Walusimbi SS, Mugerwa S (2023) Optimal substitution of black soldier fly larvae for fish in broiler chicken diets. Scientific African 20, e01636.
| Crossref | Google Scholar |
Nyakeri EM, Ogola HJO, Ayieko MA, Amimo FA (2017) Valorisation of organic waste material: growth performance of wild black soldier fly larvae (Hermetia illucens) reared on different organic wastes. Journal of Insects as Food and Feed 3(3), 193-202.
| Crossref | Google Scholar |
Opoku O, Hamidu JA, Adjei-Mensah B, Quaye B, Sasu P, Benante V, Amankrah MA, Donkoh A, Atuahene CC (2023) Alternative organic wastes as substrates and its impact on growth, proximate chemical and mineral composition of black soldier fly (Hermetia illucens) larvae for subsistent animal feed. Journal of Applied Animal Research 51(1), 564-572.
| Crossref | Google Scholar |
Ottoboni M, Spranghers T, Pinotti L, Baldi A, De Jaeghere W, Eeckhout M (2018) Inclusion of Hermetia Illucens larvae or prepupae in an experimental extruded feed: process optimisation and impact on in vitro digestibility. Italian Journal of Animal Science 17(2), 418-427.
| Crossref | Google Scholar |
Phaengphairee P, Boontiam W, Wealleans A, Hong J, Kim YY (2023) Dietary supplementation with full-fat Hermetia illucens larvae and multi-probiotics, as a substitute for antibiotics, improves the growth performance, gut health, and antioxidative capacity of weaned pigs. BMC Veterinary Research 19(1), 7.
| Crossref | Google Scholar |
Rahman MM, Mat K, Ishigaki G, Akashi R (2021) A review of okara (soybean curd residue) utilization as animal feed: nutritive value and animal performance aspects. Animal Science Journal 92(1), e13594.
| Crossref | Google Scholar |
Rampure SM, Velayudhannair K, Marimuthu N (2023) Characteristics of chitin extracted from different growth phases of black soldier fly, Hermetia illucens, fed with different organic wastes. International Journal of Tropical Insect Science 43(3), 979-987.
| Crossref | Google Scholar |
Ravi HK, Degrou A, Costil J, Trespeuch C, Chemat F, Vian MA (2020a) Effect of devitalization techniques on the lipid, protein, antioxidant, and chitin fractions of black soldier fly (Hermetia illucens) larvae. European Food Research and Technology 246(12), 2549-2568.
| Crossref | Google Scholar |
Ravi HK, Degrou A, Costil J, Trespeuch C, Chemat F, Vian MA (2020b) Larvae mediated valorization of industrial, agriculture and food wastes: biorefinery concept through bioconversion, processes, procedures, and products. Processes 8(7), 857.
| Crossref | Google Scholar |
Ribeiro N, Costa R, Ameixa OMCC (2022) The influence of non-optimal rearing conditions and substrates on the performance of the black soldier fly (Hermetia illucens). Insects 13(7), 639.
| Crossref | Google Scholar |
Romano N, Sinha A, Powell A, Fischer H (2023) Mineral composition in black soldier fly (Hermetia illucens) larvae and resulting frass from fruit and their peels. Journal of Insects as Food and Feed 9(1), 43-53.
| Crossref | Google Scholar |
Ryckebosch E, Muylaert K, Foubert I (2012) Optimization of an analytical procedure for extraction of lipids from microalgae. Journal of the American Oil Chemists’ Society 89(2), 189-198.
| Crossref | Google Scholar |
Scieuzo C, Franco A, Salvia R, Triunfo M, Addeo NF, Vozzo S, Piccolo G, Bovera F, Ritieni A, Francia AD, Laginestra A, Schmitt E, Falabella P (2023) Enhancement of fruit byproducts through bioconversion by Hermetia illucens (Diptera: Stratiomyidae). Insect Science 30(4), 991-1010.
| Crossref | Google Scholar |
Shah AA, Totakul P, Matra M, Cherdthong A, Hanboonsong Y, Wanapat M (2022) Nutritional composition of various insects and potential uses as alternative protein sources in animal diets. Animal Bioscience 35(2), 317-331.
| Crossref | Google Scholar | PubMed |
Shumo M, Khamis FM, Tanga CM, Fiaboe KKM, Subramanian S, Ekesi S, van Huis A, Borgemeister C (2019a) Influence of temperature on selected life-history traits of black soldier fly (Hermetia illucens) reared on two common urban organic waste streams in Kenya. Animals 9(3), 79.
| Crossref | Google Scholar |
Shumo M, Osuga IM, Khamis FM, Tanga CM, Fiaboe KKM, Subramanian S, Ekesi S, van Huis A, Borgemeister C (2019b) The nutritive value of black soldier fly larvae reared on common organic waste streams in Kenya. Scientific Reports 9(1), 10110.
| Crossref | Google Scholar |
Siddiqui SA, Ristow B, Rahayu T, Putra NS, Widya Yuwono N, Nisa K, Mategeko B, Smetana S, Saki M, Nawaz A, Nagdalian A (2022) Black soldier fly larvae (BSFL) and their affinity for organic waste processing. Waste Management 140, 1-13.
| Crossref | Google Scholar |
Siva Raman S, Stringer LC, Bruce NC, Chong CS (2022) Opportunities, challenges and solutions for black soldier fly larvae-based animal feed production. Journal of Cleaner Production 373, 133802.
| Crossref | Google Scholar |
Smets R, Verbinnen B, Van De Voorde I, Aerts G, Claes J, Van Der Borght M (2020) Sequential extraction and characterisation of lipids, proteins, and chitin from black soldier fly (Hermetia illucens) larvae, prepupae, and pupae. Waste and Biomass Valorization 11(12), 6455-6466.
| Crossref | Google Scholar |
Son J, Park SH, Jung HJ, You SJ, Kim BG (2023) Effects of drying methods and blanching on nutrient utilization in black soldier fly larva meals based on in vitro assays for pigs. Animals 13(5), 858.
| Crossref | Google Scholar |
Spranghers T, Ottoboni M, Klootwijk C, Ovyn A, Deboosere S, De Meulenaer B, Michiels J, Eeckhout M, De Clercq P, De Smet S (2017) Nutritional composition of black soldier fly (Hermetia illucens) prepupae reared on different organic waste substrates. Journal of the Science of Food and Agriculture 97(8), 2594-2600.
| Crossref | Google Scholar |
Tomberlin JK, Miranda C, Flint C, Harris E, Wu G (2023) Nutrients limit production of insects for food and feed: an emphasis on nutritionally essential amino acids. Animal Frontiers 13(4), 64-71.
| Crossref | Google Scholar |
Weeks EN, Baniszewski J, Gezan SA, Allan SA, Cuda JP, Stevens BR (2019) Methionine as a safe and effective novel biorational mosquito larvicide. Pest Management Science 75(2), 346-355.
| Crossref | Google Scholar |
Yakti W, Schulz S, Marten V, Mewis I, Padmanabha M, Hempel A-J, Kobelski A, Streif S, Ulrichs C (2022) The effect of rearing scale and density on the growth and nutrient composition of Hermetia illucens (L.) (Diptera: Stratiomyidae) Larvae. Sustainability 14(3), 1772.
| Crossref | Google Scholar |
Zhou JS, Liu SS, Ji H, Yu HB (2018) Effect of replacing dietary fish meal with black soldier fly larvae meal on growth and fatty acid composition of Jian carp (Cyprinus carpio var. Jian). Aquaculture Nutrition 24(1), 424-433.
| Crossref | Google Scholar |
Zulkifli NFNM, Seok-Kian AY, Seng LL, Mustafa S, Kim Y-S, Shapawi R (2022) Nutritional value of black soldier fly (Hermetia illucens) larvae processed by different methods. PLoS ONE 17(2), e0263924.
| Crossref | Google Scholar |