Grazing behaviour and nutrient intake of dairy cows is affected by milking order and daily time at pasture
M. L. Douglas

A
B
C
Abstract
The average herd size on Australian dairy farms has increased in recent decades, with herds of over 500 cows producing one-third of the nation’s annual milk.
This 16-day experiment aimed to simulate a large herd of cows progressively returning to the paddock after milking, to understand how grazing behaviour, nutrient intake and farm profit are affected when cows consistently spend prolonged periods of time away from pasture.
Forty spring-calving, Holstein-Friesian cows returned to the paddock in five groups at 45-min intervals after each milking. Cows grazed perennial ryegrass pasture offered at an allowance of 25 kg of dry matter (DM)/cow per day (to ground level), and were fed 6.0 kg of DM/cow per day of a concentrate mix in the dairy during milking. During the 5-day measurement period, grazing behaviour was measured using noseband sensors and pedometers; the n-alkane technique was employed to determine individual cow pasture dry matter intake; and the depletion of pasture mass and nutritive characteristics over a grazing session was measured.
There was no difference in daily total eating time, but cows that arrived at the paddock 135 min after the first cows had longer initial grazing bouts in the a.m. grazing session. Milk yield declined linearly as cows spent more time away from pasture, with a 6 kg/cow per day difference between the first and last cows. Higher metabolizable energy and crude protein intakes were observed in cows that had higher pasture intakes. Immediate profit was lowest for the last cows to return to the paddock by AU$2.48/cow per day.
Total daily eating time is not affected by milking order, but no change in grazing time would be able to counteract the effect of reduced pasture quantity and quality for the later-milked cows to enable them to increase their nutrient intake. The influence of milking order on milk production also leads to lower profit for cows that return to the paddock later.
Understanding how time away from pasture affects grazing behaviour and nutrient intake will allow the development of mitigation strategies to be investigated.
Keywords: dairy cows, grazing behaviour, milking order, n-alkane technique, nutrient intake, pasture intake, profit, RumiWatch.
References
ABARES (2018) Agricultural commodity statistics. Available at https://www.agriculture.gov.au/abares/research-topics/agricultural-commodities/commodity-statistics [accessed 16 December 2019]
Alvarez-Hess PS, Douglas ML, Wright MM, Norbu N, Giri K, Wales WJ, Jacobs JL, Auldist MJ (2021) Effects of herbage mass and herbage allowance on bite mass of grazing dairy cows. Animal Feed Science and Technology 278, 115011.
| Crossref | Google Scholar |
Barrett PD, Laidlaw AS, Mayne CS, Christie H (2001) Pattern of herbage intake rate and bite dimensions of rotationally grazed dairy cows as sward height declines. Grass and Forage Science 56(4), 362-373.
| Crossref | Google Scholar |
Beggs DS, Fisher AD, Jongman EC, Hemsworth PH (2015) A survey of Australian dairy farmers to investigate animal welfare risks associated with increasing scale of production. Journal of Dairy Science 98(8), 5330-5338.
| Crossref | Google Scholar | PubMed |
Beggs DS, Jongman EC, Hemsworth PH, Fisher AD (2018a) Short communication: milking order consistency of dairy cows in large Australian herds. Journal of Dairy Science 101(1), 603-608.
| Crossref | Google Scholar |
Beggs DS, Jongman EC, Hemsworth PE, Fisher AD (2018b) Implications of prolonged milking time on time budgets and lying behavior of cows in large pasture-based dairy herds. Journal of Dairy Science 101(11), 10391-10397.
| Crossref | Google Scholar |
Beggs DS, Jongman EC, Hemsworth PH, Fisher AD (2019) The effects of herd size on the welfare of dairy cows in a pasture-based system using animal- and resource-based indicators. Journal of Dairy Science 102(4), 3406-3420.
| Crossref | Google Scholar | PubMed |
Berkhout MJ, Auldist MJ, Douglas ML, Thomson AL, Giri K, Jacobs JL, Wright MM (2025) Milk yield and pasture nutrient availability associated with milking order in commercial dairy herds. Animal Production Science 65, AN24285.
| Crossref | Google Scholar |
Berry DP, McCarthy J (2012) Genetic and non-genetic factors associated with milking order in lactating dairy cows. Applied Animal Behaviour Science 136(1), 15-19.
| Crossref | Google Scholar |
Chacon E, Stobbs TH (1976) Influence of progressive defoliation of a grass sward on the eating behaviour of cattle. Australian Journal of Agricultural Research 27(5), 709-727.
| Crossref | Google Scholar |
Chilibroste P, Tamminga S, Boer H, Gibb MJ, den Dikken G (2000) Duration of regrowth of ryegrass (Lolium perenne) effects on grazing behavior, intake, rumen fill, and fermentation of lactating dairy cows. Journal of Dairy Science 83, 984-995.
| Crossref | Google Scholar | PubMed |
Chilibroste P, Soca P, Mattiauda DA, Bentancur O, Robinson PH (2007) Short term fasting as a tool to design effective grazing strategies for lactating dairy cattle: a review. Australian Journal of Experimental Agriculture 47(9), 1075-1084.
| Crossref | Google Scholar |
Chilibroste P, Gibb MJ, Soca P, Mattiauda DA (2015) Behavioural adaptation of grazing dairy cows to changes in feeding management: do they follow a predictable pattern? Animal Production Science 55(3), 328-338.
| Crossref | Google Scholar |
Cullen BR, Bullen D, Hutcheson C, Jacobs JL, Deighton MH (2017) Changes in nutritive characteristics associated with plant height, and nutrient selection by dairy cows grazing four perennial pasture grasses. Animal Production Science 57(7), 1392-1397.
| Crossref | Google Scholar |
Cullen BR, Weng H-M, Talukder S, Cheng L (2021) Cow milking order and its influence on milk production in a pasture-based automatic milking system. Animal Production Science 61(3), 306-312.
| Crossref | Google Scholar |
Dairy Australia (2019) Hay and grain reports. Available at https://www.dairyaustralia.com.au/industry/farm-inputs-and-costs/hay-and-grain-report-overview [accessed 16 December 2019]
Dairy One (2020) Forage lab analytical procedures. Available at https://dairyone.com/download/forage-forage-lab-analytical-procedures/ [accessed 28 September 2020]
Delagarde R, Peyraud JL, Delaby L, Faverdin P (2000) Vertical distribution of biomass, chemical composition and pepsin – cellulase digestibility in a perennial ryegrass sward: interaction with month of year, regrowth age and time of day. Animal Feed Science and Technology 84(2), 49-68.
| Crossref | Google Scholar |
Dias K, Garcia S, Islam M, Clark C (2019) Milk yield, milk composition, and the nutritive value of feed accessed varies with milking order for pasture-based dairy cattle. Animals 9(2), 60.
| Crossref | Google Scholar | PubMed |
Dove H, Mayes RW (1991) The use of plant wax alkanes as marker substances in studies of the nutrition of herbivores: a review. Australian Journal of Agricultural Research 42(6), 913-952.
| Crossref | Google Scholar |
Dove H, Mayes RW (2006) Protocol for the analysis of n-alkanes and other plant-wax compounds and for their use as markers for quantifying the nutrient supply of large mammalian herbivores. Nature Protocols 1, 1680-1697.
| Crossref | Google Scholar | PubMed |
Earle DF (1976) A guide to scoring dairy cow condition. Journal of Agriculture Victoria 74, 228-231.
| Google Scholar |
Earle DF, McGowan AA (1979) Evaluation and calibration of an automated rising plate meter for estimating dry matter yield of pasture. Australian Journal of Experimental Agriculture and Animal Husbandry 19(98), 337-343.
| Crossref | Google Scholar |
Fox DG, Sniffen CJ, O’Connor JD, Russell JB, Van Soest PJ (1992) A net carbohydrate and protein system for evaluating cattle diets: III. Cattle requirements and diet adequacy. Journal of Animal Science 70(11), 3578-3596.
| Crossref | Google Scholar | PubMed |
French P, O’Brien B, Shalloo L (2015) Development and adoption of new technologies to increase the efficiency and sustainability of pasture-based systems. Animal Production Science 55(7), 931-935.
| Crossref | Google Scholar |
Fulkerson WJ, McKean K, Nandra KS, Barchia IM (2005) Benefits of accurately allocating feed on a daily basis to dairy cows grazing pasture. Australian Journal of Experimental Agriculture 45(4), 331-336.
| Crossref | Google Scholar |
Garcia SC, Pedernera M, Fulkerson WJ, Horadagoda A, Nandra K (2007) Feeding concentrates based on individual cow requirements improves the yield of milk solids in dairy cows grazing restricted pasture. Australian Journal of Experimental Agriculture 47(5), 502-508.
| Crossref | Google Scholar |
GenStat 21 (2020) VSN International Ltd. Available at http://www.vsn-intl.com/
Gibb MJ, Huckle CA, Nuthall R, Rook AJ (1997) Effect of sward surface height on intake and grazing behaviour by lactating Holstein Friesian cows. Grass and Forage Science 52(3), 309-321.
| Crossref | Google Scholar |
Gibb MJ, Huckle CA, Nuthall R (1998) Effect of time of day on grazing behaviour by lactating dairy cows. Grass and Forage Science 53(1), 41-46.
| Crossref | Google Scholar |
Hills JL, Wales WJ, Dunshea FR, Garcia SC, Roche JR (2015) Invited review: an evaluation of the likely effects of individualized feeding of concentrate supplements to pasture-based dairy cows. Journal of Dairy Science 98(3), 1363-1401.
| Crossref | Google Scholar | PubMed |
Ho CKM, Auldist MJ, Wright MM, Marett LC, Malcolm B, Wales WJ (2021) Economic analysis of offering different herbage allowances to dairy cows fed a partial mixed ration. Animals 11(6), 1704.
| Crossref | Google Scholar | PubMed |
Horan B, Dillon P, Faverdin P, Delaby L, Buckley F, Rath M (2005) The interaction of strain of Holstein-Friesian cows and pasture-based feed systems on milk yield, body weight, and body condition score. Journal of Dairy Science 88(3), 1231-1243.
| Crossref | Google Scholar | PubMed |
Kennedy E, O’Donovan M, Murphy JP, O’Mara FP, Delaby L (2006) The effect of initial spring grazing date and subsequent stocking rate on the grazing management, grass dry matter intake and milk production of dairy cows in summer. Grass and Forage Science 61(4), 375-384.
| Crossref | Google Scholar |
Kennedy E, McEvoy M, Murphy JP, O’Donovan M (2009) Effect of restricted access time to pasture on dairy cow milk production, grazing behavior, and dry matter intake. Journal of Dairy Science 92(1), 168-176.
| Crossref | Google Scholar | PubMed |
King KR, Stockdale CR (1981) Milk yield of dairy cows given restricted access to water in a Mediterranean-type climate. Australian Journal of Experimental Agriculture and Animal Husbandry 21(109), 167-171.
| Crossref | Google Scholar |
Kolver ES, Muller LD (1998) Performance and nutrient intake of high producing Holstein cows consuming pasture or a total mixed ration. Journal of Dairy Science 81, 1403-1411.
| Crossref | Google Scholar | PubMed |
Lewis CD, Smith KF, Jacobs JL, Ho CKM, Leddin CM, Malcolm B (2020) Using a two-price market value method to value extra pasture DM in different seasons. Agricultural Systems 178, 102729.
| Crossref | Google Scholar |
Li Z, Cheng L, Cullen B (2021) Validation and use of the RumiWatch noseband sensor for monitoring grazing behaviours of lactating dairy cows. Dairy 2(1), 104-111.
| Crossref | Google Scholar |
Liu Z, Ezernieks V, Bui AD, Krill C, Wright M, Rochfort S (2022) A simplified alkane analysis method for the determination of herbage intake by dairy cows. Agronomy 12(11), 2720.
| Crossref | Google Scholar |
Mayes RW, Lamb CS, Colgrove PM (1986) The use of dosed and herbage n-alkanes as markers for the determination of herbage intake. The Journal of Agricultural Science 107, 161-170.
| Crossref | Google Scholar |
Newman JA, Parsons AJ, Penning PD (1994) A note on the behavioural strategies used by grazing animals to alter their intake rates. Grass and Forage Science 49(4), 502-505.
| Crossref | Google Scholar |
Norbu N, Alvarez-Hess PS, Leury BJ, Wright MM, Douglas ML, Moate PJ, Williams SRO, Marett LC, Garner JB, Wales WJ, Auldist MJ (2021) Assessment of RumiWatch noseband sensors for the quantification of ingestive behaviors of dairy cows at grazing or fed in stalls. Animal Feed Science and Technology 280, 115076.
| Crossref | Google Scholar |
O’Driscoll K, O’Brien B, Gleeson D, Boyle L (2010) Milking frequency and nutritional level affect grazing behaviour of dairy cows: a case study. Applied Animal Behaviour Science 122(2–4), 77-83.
| Crossref | Google Scholar |
Pérez-Ramírez E, Delagarde R, Delaby L (2008) Herbage intake and behavioural adaptation of grazing dairy cows by restricting time at pasture under two feeding regimes. Animal 2(9), 1384-1392.
| Crossref | Google Scholar | PubMed |
Pérez-Ramírez E, Peyraud JL, Delagarde R (2009) Restricting daily time at pasture at low and high pasture allowance: Effects on pasture intake and behavioral adaptation of lactating dairy cows. Journal of Dairy Science 92(7), 3331-3340.
| Crossref | Google Scholar | PubMed |
Perojo A, Garcia-Rodrigeuz A, Arranz J, Oregui LM (2005) Effects of time spent on pastures on milk yield, body reserves, herbage intake and grazing behaviour. Options Méditerranéennes 67, 275-279.
| Google Scholar |
Polikarpus A, Kaart T, Mootse H, De Rose G, Arney D (2015) Influences of various factors on cows’ entrance order into the milking parlour. Applied Animal Behaviour Science 166, 20-24.
| Crossref | Google Scholar |
Rathore AK (1982) Order of cow entry at milking and its relationships with milk yield and consistency of the order. Applied Animal Ethology 8(1–2), 45-52.
| Crossref | Google Scholar |
Rook AJ, Huckle CA, Penning PD (1994) Effects of sward height and concentrate supplementation on the ingestive behaviour of spring-calving dairy cows grazing grass-clover swards. Applied Animal Behaviour Science 40(2), 101-112.
| Crossref | Google Scholar |
Scott BA, Camacho A, Golder H, Molfino J, Kerrisk KL, Lean I, Garcia SC, Chaves AV, Hall E, Clark CEF (2014) The nutritive value of pasture ingested by dairy cows varies within a herd. In ‘Proceedings of the 5th Australasian dairy science symposium’, 19–21 November, Hamilton, New Zealand. pp. 343–346. Available at https://australasiandairyscience.com/wp-content/uploads/2022/11/ADSS2014_FULL-proceedings.pdf
Scott Mitchell R, Sherlock RA, Smith LA (1996) An investigation into the use of machine learning for determining oestrus in cows. Computers and Electronics in Agriculture 15(3), 195-213.
| Crossref | Google Scholar |
Soffié M, Thinès G, De Marneffe G (1976) Relation between milking order and dominance value in a group of dairy cows. Applied Animal Ethology 2(3), 271-276.
| Crossref | Google Scholar |
Stockdale CR (1999) The nutritive characteristics of herbage consumed by grazing dairy cows affect milk yield responses obtained from concentrate supplementation. Australian Journal of Experimental Agriculture 39(4), 379-387.
| Crossref | Google Scholar |
Tyrrell HR, Reid JT (1965) Prediction of the energy value of cow’s milk. Journal of Dairy Science 48, 1215-1223.
| Crossref | Google Scholar | PubMed |
Van Vuuren AM, Van der Koelen CJ, Vroons-de Bruin J (1986) Influence of level and composition of concentrate supplements on rumen fermentation patterns of grazing dairy cows. Netherlands Journal of Agricultural Science 34(4), 457-467.
| Crossref | Google Scholar |
Wales WJ, Kolver ES (2017) Challenges of feeding dairy cows in Australia and New Zealand. Animal Production Science 57(7), 1366-1383.
| Crossref | Google Scholar |
Wales WJ, Doyle PT, Dellow DW (1998) Dry matter intake and nutrient selection by lactating cows grazing irrigated pastures at different pasture allowances in summer and autumn. Australian Journal of Experimental Agriculture 38(5), 451-460.
| Crossref | Google Scholar |
Wales WJ, Doyle PT, Stockdale CR, Dellow DW (1999) Effects of variations in herbage mass, allowance, and level of supplement on nutrient intake and milk production of dairy cows in spring and summer. Australian Journal of Experimental Agriculture 39(2), 119-130.
| Crossref | Google Scholar |
Werner J, Umstatter C, Kennedy E, Grant J, Leso L, Geoghegan A, Shalloo L, Shick M, O’Brien B (2019) Identification of possible cow grazing behaviour indicators for restricted grass availability in a pasture-based spring calving dairy system. Livestock Science 220, 74-82.
| Crossref | Google Scholar |
Wilkinson JM, Less MRF, Rivero MJ, Chamberlain AT (2020) Some challenges and opportunities for grazing dairy cows on temperate pastures. Grass and Forage Science 75(1), 1-17.
| Crossref | Google Scholar | PubMed |
Wright MM, Douglas ML, Alvarez-Hess PS, Russo VM, Morris GL, Hannah MC, Giri K, Wales WJ, Auldist MJ (2025) Effects of time away from pasture on milk production and dry matter intake in dairy cattle. Animal Production Science 65, AN25027.
| Crossref | Google Scholar |