Fate of 15N-labelled urea under cotton and maize as affected by urease and nitrification inhibitors, and application method
Tariq Mahmood A , Muhammad Akhtar
A * , Rehmat Ali A , Zafar Iqbal A , Saira Butt B , Fariha Malik B and Muhammad Yaqub A
A
B
Abstract
The use of urease and nitrification inhibitors is an important mitigation strategy to reduce fertilizer-N loss from croplands. However, limited information exists on the performance of urease and nitrification inhibitors in alkaline calcareous soils under warm climate in the Indo-Gangetic plains.
The study aimed at elucidating the effects of urease inhibitor N-(n-butyl) thiophosphoric triamide (NBPT) and nitrification inhibitor 4-amino-1,2,4-triazole (ATC) on the fate of 15N-urea applied to cotton and maize grown in an alkaline calcareous soil under greenhouse conditions.
Four fertilizer treatments (15N-urea, 15N-urea + NBPT, 15N-urea + ATC, and 15N-urea + NBPT + ATC) were applied either by the conventional method (granules surface broadcast in splits followed by incorporation/irrigation) or in subsurface bands (granules or liquid; all applied at sowing). Cotton plants were grown to maturity (197 days) whereas maize was harvested at the tasseling stage (61 days). Soil and different plant components were analyzed for total N and 15N contents.
With the conventional application method, NBPT reduced urea-N loss under cotton, whereas ATC increased the loss both under cotton and maize. The lowest N loss was recorded with urea + NBPT applied as liquid in subsurface bands.
In alkaline calcareous soils, ATC may not be effective in reducing urea-N loss under crops exposed to high summer temperatures (average, 33.7–35.7°C), whereas high urea-N loss under these crops can be substantially reduced by subsurface banding of urea along with NBPT.
The results warrant further studies to explore appropriate fertilizer-N management practises to reduce fertilizer-N loss under agro-climatic conditions in the Indo-Gangetic plains.
Keywords: 15N-urea, alkaline calcareous soils, ATC, cotton, Indo-Gangetic plains, maize, NBPT, urea application methods, warm climate.
References
Afshar RK, Ruiyu L, Mohammad YA, Chen C (2018) Agronomic effects of urease and nitrification inhibitors on ammonia volatilization and nitrogen utilization in a dryland farming system: field and laboratory investigation. Journal of Cleaner Production 172, 4130-4139.
| Crossref | Google Scholar |
Akhtar M, Naeem A (2012) Reduction in ammonia loss by applying urea in combination with phosphate sources. Communications in Soil Science and Plant Analysis 43(15), 2043-2049.
| Crossref | Google Scholar |
Ali R, Iqbal J, Tahir GR, Mahmood T (2008) Effect of 3,5-dimethylpyrazole and nitrapyrin on nitrification under high soil temperature. Pakistan Journal of Botany 40(3), 1053-1062.
| Google Scholar |
Ali R, Iqbal J, Robab U, Mahmood T (2009) Effect of dicyandiamide on microbial activity in the rhizosphere and bulk soils under cotton. Pakistan Journal of Botany 41(3), 1401-1409.
| Google Scholar |
Ali R, Kanwal HY, Iqbal Z, Yaqub M, Khan JA, Mahmood T (2012) Evaluation of some nitrification inhibitors at different temperatures under laboratory conditions. Soil and Environment 31(2), 134-145.
| Google Scholar |
Artola E, Cruchaga S, Ariz I, Moran JF, Garnica M, Houdusse F, Mina JMG, Irigoyen I, Lasa B, Aparicio-Tejo PM (2011) Effect of N-(n-butyl) thiophosphoric triamide on urea metabolism and the assimilation of ammonium by Triticum aestivum L. Plant Growth Regulation 63, 73-79.
| Crossref | Google Scholar |
Aulakh MS, Rennie DA, Paul EA (1983) Field studies on gaseous nitrogen losses from soils under continuous wheat versus a wheat-fallow rotation. Plant and Soil 75, 15-27.
| Crossref | Google Scholar |
Aulakh MS, Singh K, Doran J (2001) Effects of 4-amino 1,2,4-triazole, dicyandiamide and encapsulated calcium carbide on nitrification inhibition in a subtropical soil under upland and flooded conditions. Biology and Fertility of Soils 33, 258-263.
| Crossref | Google Scholar |
Azam F, Simmons FW, Mulvaney RL (1993) Mineralization of N from plant residues and its interaction with native soil N. Soil Biology and Biochemistry 25(12), 1787-1792.
| Crossref | Google Scholar |
Barakat M, Cheviron B, Angulo-Jaramillo R (2016) Influence of the irrigation technique and strategies on the nitrogen cycle and budget: a review. Agricultural Water Management 178, 225-238.
| Crossref | Google Scholar |
Barton L, McLay CDA, Schipper LA, Smith CT (1999) Annual denitrification rates in agricultural and forest soils: a review. Australian Journal of Soil Research 37(6), 1073-1094.
| Crossref | Google Scholar |
Bouwman AF, Boumans LJM, Batjes NH (2002) Estimation of global NH3 volatilization loss from synthetic fertilizers and animal manure applied to arable lands and grasslands. Global Biogeochemical Cycles 16(2), 8-1-8-14.
| Crossref | Google Scholar |
Bremner JM, Chai HS (1986) Evaluation of N-butyl phosphorothioic triamide for retardation of urea hydrolysis in soil. Communications in Soil Science and Plant Analysis 17(3), 337-351.
| Crossref | Google Scholar |
Bremner JM, Mulvaney CS (1982) Nitrogen—total. In ‘Methods of soil analysis, Vol. 2’. Agronomy Monograph 9. (Eds AL Page, RH Miller, DR Keeney) pp. 595–662. (American Society of Agronomy: Madison) 10.2134/agronmonogr9.2.2ed.c31
Cantarella H, Otto R, Soares JR, De Brito Silva AG (2018) Agronomic efficiency of NBPT as a urease inhibitor: a review. Journal of Advanced Research 13, 19-27.
| Crossref | Google Scholar | PubMed |
Chen D, Suter H, Islam A, Edis R, Freney JR, Walker CN (2008) Prospects of improving efficiency of fertiliser nitrogen in Australian agriculture: a review of enhanced efficiency fertilisers. Australian Journal of Soil Research 46(4), 289-301.
| Crossref | Google Scholar |
Da Silva MJ, Magalhães HCJ, Graziano Magalhães PS (2017) Liquid fertilizer application to ratoon cane using a soil punching method. Soil and Tillage Research 165, 279-285.
| Crossref | Google Scholar |
Di HJ, Cameron KC (2004) Treating grazed pasture soil with a nitrification inhibitor, eco-n™, to decrease nitrate leaching in a deep sandy soil under spray irrigation – a lysimeter study. New Zealand Journal of Agricultural Research 47(3), 351-361.
| Crossref | Google Scholar |
Edmeades DC (2004) ‘Nitrification and urease inhibitors.’ Environmental Publication 2004/11. (Environment Bay of Plenty: Whakatāne) 10.1080/00288233.2004.9513566
Erisman JW, Bleeker A, Galloway J, Sutton MS (2007) Reduced nitrogen in ecology and the environment. Environmental Pollution 150(1), 140-149.
| Crossref | Google Scholar | PubMed |
Gioacchini P, Nastri A, Marzadori C, Giovannini C, Antisari LV, Gessa C (2002) Influence of urease and nitrification inhibitors on N losses from soils fertilized with urea. Biology and Fertility of Soils 36, 129-135.
| Crossref | Google Scholar |
Hauck RD (1982) Nitrogen-isotope-ratio analysis. In ‘Methods of soil analysis, Vol. 2’. Agronomy Monograph 9. (Eds AL Page, RH Miller, DR Keeney) pp. 735–779. (American Society of Agronomy: Madison) 10.2134/agronmonogr9.2.2ed.c36.
Irigoyen I, Nuro J, Azpilikueta M, Aparicio-Tejo P, Lamsfus C (2003) Ammonium oxidation kinetics in the presence of nitrification inhibitors DCD and DMPP at various temperatures. Australian Journal of Soil Research 41(6), 1177-1183.
| Crossref | Google Scholar |
Kawakami EM, Oosterhuis DM, Snider JL, Mozaffari M (2012) Physiological and yield responses of field-grown cotton to application of urea with the urease inhibitor NBPT and the nitrification inhibitor DCD. European Journal of Agronomy 43, 147-154.
| Crossref | Google Scholar |
Kim D-G, Saggar S, Roudier P (2012) The effect of nitrification inhibitors on soil ammonia emissions in nitrogen managed soils: a meta-analysis. Nutrient Cycling in Agroecosystems 93, 51-64.
| Crossref | Google Scholar |
Klimczyk M, Siczek A, Schimmelpfennig L (2021) Improving the efficiency of urea-based fertilization leading to reduction in ammonia emission. Science of the Total Environment 771, 145483.
| Crossref | Google Scholar |
Kroeze C (1994) Nitrous oxide and global warming. Science of the Total Environment 143(2–3), 193-209.
| Crossref | Google Scholar |
Krol DJ, Forrestal JP, Wall D, Lanigan JG, Sanz-Gomez J, Richards KG (2020) Nitrogen fertilisers with urease inhibitors reduce nitrous oxide and ammonia losses, while retaining yield in temperate grassland. Science of the Total Environment 725, 138329.
| Crossref | Google Scholar |
Lin B-L, Sakoda A, Shibasaki R, Suzuki M (2001) A modelling approach to global nitrate leaching caused by anthropogenic fertilisation. Water Research 35(8), 1961-1968.
| Crossref | Google Scholar | PubMed |
Liu S, Pubu C, Zhu Y, Hao W, Zhang G, Han J (2023) Optimizing nitrogen application depth can improve crop yield and nitrogen uptake – a global meta-analysis. Field Crops Research 295, 108895.
| Crossref | Google Scholar |
Ma L, Ruan J (2021) Deep placement of nitrogen fertilizer in autumn improves N utilization by spring tea (Camellia sinensis L.). Journal of Plant Nutrition and Soil Science 184(4), 430-438.
| Crossref | Google Scholar |
Ma Q, Tang H, Rengel Z, Shen J (2013) Banding phosphorus and ammonium enhances nutrient uptake by maize via modifying root spatial distribution. Crop & Pasture Science 64(10), 965-975.
| Crossref | Google Scholar |
Mahmood T, Malik KA, Shamsi SRA, Sajjad MI (1998) Denitrification and total N losses from an irrigated sandy-clay loam under maize-wheat cropping system. Plant and Soil 199, 239-250.
| Crossref | Google Scholar |
Mahmood T, Ali R, Sajjad MI, Chaudhri MB, Tahir GR, Azam F (2000) Denitrification and total fertilizer-N losses from an irrigated cotton field. Biology and Fertility of Soils 31, 270-278.
| Crossref | Google Scholar |
Mahmood T, Woitke M, Gimmler H, Kaiser WM (2002) Sugar exudation by roots of kallar grass [Leptochloa fusca (L.) Kunth] is strongly affected by the nitrogen source. Planta 214, 887-894.
| Crossref | Google Scholar | PubMed |
Mahmood T, Kaiser WM, Ali R, Ashraf M, Gulnaz A, Iqbal Z (2005) Ammonium versus nitrate nutrition of plants stimulates microbial activity in the rhizosphere. Plant and Soil 277, 233-243.
| Crossref | Google Scholar |
Mahmood T, Ali R, Latif Z, Ishaque W (2011) Dicyandiamide increases the fertilizer N loss from an alkaline calcareous soil treated with 15N-labelled urea under warm climate and under different crops. Biology and Fertility of Soils 47, 619-631.
| Crossref | Google Scholar |
Mahmood T, Ali R, Lodhi A, Sajid M (2017) 4-Amino-1,2,4-triazole can be more effective than commercial nitrification inhibitors at high soil temperatures. Soil Research 55(7), 715-722.
| Crossref | Google Scholar |
Mahmood T, Ali R, Yaqub M (2023) Transformations of surface-applied urea in an alkaline calcareous soil as affected by urease inhibitor N-(n-butyl) thiophosphoric triamide at different temperatures. Soil and Environment 42(2), 216-222.
| Crossref | Google Scholar |
McBeath TM, Gupta VV, Llewellyn RS, Mason SD, Davoren CW, Correll RL, Jones B, Whitbread AM (2019) Combined application of nitrogen and phosphorus to enhance nitrogen use efficiency and close the wheat yield gap on varying soils in semi-arid conditions. Journal of Agronomy and Crop Science 205(6), 635-646.
| Crossref | Google Scholar |
Menegat S, Ledo A, Tirado R (2022) Greenhouse gas emissions from global production and use of nitrogen synthetic fertilisers in agriculture. Scientific Reports 12, 14490.
| Crossref | Google Scholar |
Motasim AM, Samsuri AW, Abdul Sukor AS, Adibah AM (2021a) Gaseous nitrogen losses from tropical soils with liquid or granular urea fertilizer application. Sustainability 13(6), 3128.
| Crossref | Google Scholar |
Motasim AM, Samsuri AW, Abdul Sukor AS, Adibah AM (2021b) Nitrogen dynamics in tropical soils treated with liquid and granular urea fertilizers. Agriculture 11(6), 546.
| Crossref | Google Scholar |
Motasim AM, Samsuri AW, Nabayi A, Akter A, Haque MA, Abdul Sukor AS, Adibah AM (2024) Urea application in soil: processes, losses, and alternatives ‒ a review. Discover Agriculture 2, 42.
| Crossref | Google Scholar |
Murphy JA, Zaurov DE (1994) Shoot and root growth response of perennial ryegrass to fertilizer placement depth. Agronomy Journal 86(5), 828-832.
| Crossref | Google Scholar |
Pelster DE, Chantigny MH, Angers DA, Bertrand N, MacDonald JD, Rochette P (2018) Can soil clay content predict ammonia volatilization losses from subsurface-banded urea in eastern Canadian soils? Canadian Journal of Soil Science 98(3), 556-565.
| Crossref | Google Scholar |
Qi X, Wu W, Shah F, Peng S, Huang J, Cui K, Liu H, Nie L (2012) Ammonia volatilization from urea-application influenced germination and early seedling growth of dry direct-seeded rice. The Scientific World Journal 2012(1), 857472.
| Crossref | Google Scholar |
Reddy GR (1964) Effect of mixing varying quantities of dicyandiamide with ammonium fertilizers on nitrification of ammonia in soils. Canadian Journal of Soil Science 44(2), 254-259.
| Crossref | Google Scholar |
Rochester I, Constable G, Saffigna P (1996) Effective nitrification inhibitors may improve fertilizer recovery in irrigated cotton. Biology and Fertility of Soils 23, 1-6.
| Crossref | Google Scholar |
Rochette P, Angers DA, Chantigny MH, MacDonald JD, Gasser MO, Bertrand N (2009) Reducing ammonia volatilization in a no-till soil by incorporating urea and pig slurry in shallow bands. Nutrient Cycling in Agroecosystems 84, 71-80.
| Crossref | Google Scholar |
Rochette P, Angers DA, Chantigny MH, Gasser M-O, MacDonald JD, Pelster DE, Bertrand N (2013a) NH3 volatilization, soil NH4+ concentration and soil pH following subsurface banding of urea at increasing rates. Canadian Journal of Soil Science 93(2), 261-268.
| Crossref | Google Scholar |
Rochette P, Angers DA, Chantigny MH, Gasser M-O, MacDonald JD, Pelster DE, Bertrand N (2013b) Ammonia volatilization and nitrogen retention: how deep to incorporate urea? Journal of Environmental Quality 42(6), 1635-1642.
| Crossref | Google Scholar |
Ryden JC, Lund LJ (1980) Nature and extent of directly measured denitrification losses from some irrigated vegetable crop production units. Soil Science Society of America Journal 44(3), 505-511.
| Crossref | Google Scholar |
Skorupka M, Nosalewicz A (2021) Ammonia volatilization from fertilizer urea – a new challenge for agriculture and industry in view of growing global demand for food and energy crops. Agriculture 11(9), 822.
| Crossref | Google Scholar |
Smith LC, Monaghan RM, Ledgard SF, Catto WD (2005) The effectiveness of different nitrification inhibitor formulations in limiting nitrate accumulation in a Southland pastoral soil. New Zealand Journal of Agricultural Research 48(4), 517-529.
| Crossref | Google Scholar |
Soares JR, Cantarella H, De Campos Menegale ML (2012) Ammonia volatilization losses from surface-applied urea with urease and nitrification inhibitors. Soil Biology and Biochemistry 52, 82-89.
| Crossref | Google Scholar |
Sogbedji JM, Van Es HM, Yang CL, Geohring LD, Magdoff FR (2000) Nitrate leaching and nitrogen budget as affected by maize nitrogen rate and soil type. Journal of Environmental Quality 29(6), 1813-1820.
| Crossref | Google Scholar |
Ti C, Xia L, Chang SX, Yan X (2019) Potential for mitigating global agricultural ammonia emission: a meta-analysis. Environmental Pollution 245, 141-148.
| Crossref | Google Scholar | PubMed |
Tian H, Yang J, Xu R, Lu C, Canadell JG, Davidson EA, Jackson RB, Arneth A, Chang J, Ciais P, Gerber S, Ito A, Joos F, Lienert S, Messina P, Olin S, Pan S, Peng C, Saikawa E, Thompson RL, Vuichard N, Winiwarter W, Zaehle S, Zhang B (2019) Global soil nitrous oxide emissions since the preindustrial era estimated by an ensemble of terrestrial biosphere models: magnitude, attribution, and uncertainty. Global Change Biology 25(2), 640-659.
| Crossref | Google Scholar | PubMed |
Vallis I, Catchpoole VR, Hughes RM, Myers RJK, Ridge DR, Weier KL (1996) Recovery in plants and soils of 15N applied as subsurface bands of urea to sugarcane. Australian Journal of Agricultural Research 47(3), 355-370.
| Crossref | Google Scholar |
Van der Eerden L, De Vries W, Van Dobben H (1998) Effects of ammonia deposition on forests in the Netherlands. Atmospheric Environment 32(3), 525-532.
| Crossref | Google Scholar |
Van Dijk W, Brouwer G (1998) Nitrogen recovery and dry matter production of silage maize (Zea mays L.) as affected by subsurface band application of mineral nitrogen fertilizer. Netherlands Journal of Agricultural Science 46(2), 139-155.
| Crossref | Google Scholar |
Walsh OS, Christiaens RJ (2016) Relative efficacy of liquid nitrogen fertilizers in dryland spring wheat. International Journal of Agronomy 2016(1), 6850672.
| Crossref | Google Scholar |
Wolt JD (2004) A meta-evaluation of nitrapyrin agronomic and environmental effectiveness with emphasis on corn production in the Midwestern USA. Nutrient Cycling in Agroecosystems 69, 23-41.
| Crossref | Google Scholar |
Xu Y, Xu Z, Cai Z, Reverchon F (2013) Review of denitrification in tropical and subtropical soils of terrestrial ecosystems. Journal of Soils and Sediments 13, 699-710.
| Crossref | Google Scholar |
Zerulla W, Barth T, Dressel J, Erhardt K, Von Locquenghien KH, Pasda G, Rädle M, Wissemeier A (2001) 3,4-Dimethylpyrazole phosphate (DMPP) – a new nitrification inhibitor for agriculture and horticulture. Biology and Fertility of Soils 34, 79-84.
| Crossref | Google Scholar |
