Effect of dietary energy intake on erythrocytic antioxidant defence in growing lambs fed a wheat straw-based diet
Vijay Kumar Singh A C , Ashok Kumar Pattanaik A D E , Kusumakar Sharma A and Mohini Saini B
+ Author Affiliations
- Author Affiliations
A Centre of Advanced Studies in Animal Nutrition, Indian Veterinary Research Institute, Izatnagar 243 122, India.
B Division of Biochemistry, Indian Veterinary Research Institute, Izatnagar 243 122, India.
C Present address: College of Veterinary Science and Animal Husbandry, Narendra Dev University of Agriculture and Technology, Kumarganj, Faizabad 224 229, India.
D Present address: School of Veterinary and Animal Sciences, University of Adelaide, Roseworthy, SA 5371, Australia.
E Corresponding author. Email: akpattanaik1@gmail.com
Animal Production Science 51(7) 642-649 https://doi.org/10.1071/AN10098
Submitted: 17 June 2010 Accepted: 24 March 2011 Published: 27 June 2011
Abstract
Twenty-four Muzaffarnagari lambs (~8 months, 26.56 ± 2.04 kg), consisting 12 each of male and female, were used for ascertaining the effect of dietary energy restriction on the erythrocytic antioxidant defence including lipid peroxidation. The lambs, allotted randomly into three equal groups, were fed on wheat straw-based diets to provide 100, 80 and 70% of calculated metabolisable energy (ME) requirements. Bodyweight gain and feed intake were recorded. Blood samples were collected at the start and thereafter at 60-day intervals during 180 days of experimental duration and analysed for malonyl dialdehyde (MDA), reduced glutathione (GSH) and total thiol groups in addition to catalase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and glutathione S-transferase. Dietary treatments imparted significant (P < 0.001) effects on feed intake and average daily gain. The haemoglobin and haematocrit contents in blood reduced significantly (P < 0.001) on reduction in dietary energy levels. The dietary alterations elicited no change in the activities of SOD, catalase and glutathione S-transferase, but reduced activities of GSH-Px (P < 0.001) and GSH (P = 0.133) were evident on feeding the diet with 70% ME. Concentrations of total thiols decreased (P < 0.001) with reduced energy level. Both the energy-restricted groups exhibited a significant (P < 0.001) increase in MDA, indicative of increased lipid peroxidation. It was concluded that long-term energy malnutrition on a wheat straw-based diet reduces the erythrocytic antioxidant defence in growing lambs.
Additional keywords: glutathione, lipid peroxidation, oxidative stress, sheep.
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