In vitro rumen gas production kinetics, hydrocyanic acid concentration and fermentation characteristics of fresh cassava root and feed block sulfur concentration
Gamonmas Dagaew A , Anusorn Cherdthong A C , Metha Wanapat A and Pin Chanjula BA Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, 123 Moo 16 Mittraphap Road, Nai-Muang, Muang District, Khon Kaen 40002, Thailand.
B Department of Animal Science, Faculty of Natural Resources, Prince of Songkla University, 15 Karnjanavanich Road, Hat Yai, Songkhla 90110, Thailand.
C Corresponding author. Email: anusornc@kku.ac.th
Animal Production Science 60(5) 659-664 https://doi.org/10.1071/AN18784
Submitted: 21 December 2018 Accepted: 31 July 2019 Published: 13 February 2020
Abstract
Context: Feeding ruminants with fresh cassava roots (FCR) is limited because they have a high concentration of hydrocyanic acid (HCN). Thus, it was hypothesised that receiving a feed block containing high sulfur (FBS) would reduce hydrocyanic acid (HCN) in FCR and improve rumen fermentation and nutrient digestibility in animals.
Aims: The goal of the present work was to study the influence of the ratio of FCR to rice straw (RS) together with FBS on kinetics of gas production, HCN concentration, fermentation characteristics and nutrient digestibility, using in vitro technique.
Methods: The experimental design was a 4 × 2 factorial arrangement in a completely randomised design, with three replications per treatment. Factor A was the FCR to RS ratio, which was 100 : 0, 60 : 40, 40 : 60 or 0 : 100. Factor B was sulfur, which was provided as two concentrations in FBS (2% and 4% DM). Gas production was recorded after incubation, at 0, 0.5, 1, 2, 4, 6, 8, 12, 18, 24, 48, 72 and 96 h. Fermentation liquor was collected and determined for kinetics of gas production, HCN concentration, fermentation characteristics and nutrient digestibility.
Key results: Cassava root to RS ratio influenced the cumulative gas production after 96 h. Inclusion of sulfur in the FBS at 4% increased the cumulative gas production, when compared with inclusion at 2%. The gas production from degradable fraction (b) and the rate of gas production (c) were significantly different among the treatments with different FCR : RS ratios, whereas there was no difference between using 2% and 4% sulfur in the FBS. The HCN concentration in fermentation liquor increased with an increasing proportion of FCR. Furthermore, inclusion of sulfur in the feed block at 4% reduced HCN concentration by 42.8%, when compared with inclusion at 2% (P < 0.05). Ammonia-nitrogen concentration was significantly different among the FCR : RS-ratio treatments and was reduced when the proportion of FCR was decreased (P < 0.05). In vitro digestibility was significantly increased with an increasing proportion of FCR. Increasing the proportion of FCR with 4% of sulfur in the FBS significantly increased in vitro DM digestibility, compared with 2% sulfur. Increasing the FCR : RS ratio with 4% of sulfur in the FBS increased the proportion of propionic acid (P < 0.05).
Conclusions: Using a high FCR : RS ratio (100 : 0 or 60 : 40) with 4% sulfur in the FBS enhanced kinetics of gas production, propionic molar proportion, nutrient digestibility, and HCN detoxification by rumen microbes in an in vitro trial.
Implications: An in vivo study should be encouraged to verify the results and obtain more data.
Additional keywords: in vitro techniquemineral, ruminant, thiocyanate, tropical feed resource.
References
Aminlari M (2006) Distribution of the cyanide metabolizing enzyme rhodanese in different tissues of domestic animals. Journal of Veterinary Pharmacology and Therapeutics 29, 128| Distribution of the cyanide metabolizing enzyme rhodanese in different tissues of domestic animals.Crossref | GoogleScholarGoogle Scholar |
AOAC (1995) Animal feeds. In ‘Official method of analysis’. 16th edn. (Ed. PA Cunniff) (Association of Official Analytical Chemists: VA, USA)
Cherdthong A, Wanapat M, Rakwongrit D, Khota W, Khantharin S, Tangmutthapattharakun G, Kang S, Foiklang S, Phesatcha K (2014) Supplementation effect with slow-release urea in feed blocks for Thai beef cattle-nitrogen utilization, blood biochemistry and hematology. Tropical Animal Health and Production 46, 293–298.
| Supplementation effect with slow-release urea in feed blocks for Thai beef cattle-nitrogen utilization, blood biochemistry and hematology.Crossref | GoogleScholarGoogle Scholar | 24105031PubMed |
Cherdthong A, Khonkhaeng B, Seankamsorn A, Supapong C, Wanapat M, Gunun N, Gunun P, Chanjula P, Polyorach S (2018) Effects of feeding fresh cassava root with high-sulfur feed block on feed utilization, rumen fermentation, and blood metabolites in Thai native cattle. Tropical Animal Health and Production 50, 1365–1371.
| Effects of feeding fresh cassava root with high-sulfur feed block on feed utilization, rumen fermentation, and blood metabolites in Thai native cattle.Crossref | GoogleScholarGoogle Scholar | 29536317PubMed |
Huang H, Yie S, Liu Y, Wang C, Cai Z, Zhang W, Lan J, Huang X, Luo L, Cai K, Hou R, Zhang Z (2016) Dietary resources shape the adaptive changes of cyanide detoxification function in giant panda (Ailuropoda melanoleuca). Scientific Reports 6, 34700
| Dietary resources shape the adaptive changes of cyanide detoxification function in giant panda (Ailuropoda melanoleuca).Crossref | GoogleScholarGoogle Scholar | 27703267PubMed |
Lambert JL, Ramasamy J, Paukstelis JF (1975) Stable reagents for the colorimetric determination of cyanide by modified Konig reactions. Analytical Chemistry 47, 916–918.
| Stable reagents for the colorimetric determination of cyanide by modified Konig reactions.Crossref | GoogleScholarGoogle Scholar |
Menke KH, Steingass H (1988) Estimation of the energetic feed value obtained from chemical analysis and gas production using rumen fluid. Animal Research and Development 28, 7–55.
Nguyen TL, Ogle RB, Preston TR (1997) Cassava root silage for crossbred pigs under village conditions in Central Vietnam. Livestock Research for Rural Development 9, 2
Ørskov ER, McDonald I (1979) The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. The Journal of Agricultural Science 92, 499–503.
| The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage.Crossref | GoogleScholarGoogle Scholar |
Promkot C, Wanapat M (2009) Effect of elemental sulfur supplementation on rumen environment parameters and utilization efficiency of fresh cassava foliage and cassava hay in dairy cattle. Asian-Australasian Journal of Animal Sciences 22, 1366–1376.
| Effect of elemental sulfur supplementation on rumen environment parameters and utilization efficiency of fresh cassava foliage and cassava hay in dairy cattle.Crossref | GoogleScholarGoogle Scholar |
Promkot C, Wanapat M, Wachirapakorn C, Navanukraw C (2007) Influence of sulphur on fresh cassava foliage and cassava hay incubate in rumen fluid of beef cattle. Asian-Australasian Journal of Animal Sciences 20, 1424–1432.
| Influence of sulphur on fresh cassava foliage and cassava hay incubate in rumen fluid of beef cattle.Crossref | GoogleScholarGoogle Scholar |
Samuel M, Sagathewan S, Thomas J, Mathen G (1997) An HPLC method for estimation of volatile fatty acids of ruminal fluid. The Indian Journal of Animal Sciences 67, 805–811.
Sarturi JO, Erickson GE, Klopfenstein TJ, Rolfe KM, Buckner CD, Luebbe MK (2013) Impact of source of sulfur on ruminal hydrogen sulfide and logic for the ruminal available sulfur for reduction concept. Journal of Animal Science 91, 3352–3359.
| Impact of source of sulfur on ruminal hydrogen sulfide and logic for the ruminal available sulfur for reduction concept.Crossref | GoogleScholarGoogle Scholar | 23658335PubMed |
SAS Institute Inc. (1996) ‘SAS/STAT user’s guide: version 6.’ 12.4th edn. (SAS Institute Inc.: Cary, NC, USA)
Tilley JMA, Terry RA (1963) A two-stage technique for the digestion of forage crops. Journal of the British Grassland Society 18, 104–111.
| A two-stage technique for the digestion of forage crops.Crossref | GoogleScholarGoogle Scholar |
Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
| Methods for dietary fiber neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar | 1660498PubMed |
Wanapat M, Kang S (2015) Cassava chip (Manihot esculenta Crantz) as an energy source for ruminant feeding. Animal Nutrition 1, 266–270.
| Cassava chip (Manihot esculenta Crantz) as an energy source for ruminant feeding.Crossref | GoogleScholarGoogle Scholar | 29767011PubMed |
Wanapat M, Khampa S (2007) Effect of levels of supplementation of concentrate containing high levels of cassava chip on rumen ecology, microbial N supply and digestibility of nutrients in beef cattle. Asian-Australasian Journal of Animal Sciences 20, 75–81.
| Effect of levels of supplementation of concentrate containing high levels of cassava chip on rumen ecology, microbial N supply and digestibility of nutrients in beef cattle.Crossref | GoogleScholarGoogle Scholar |
Wheeler JL, Hedges DA, Till AR (1975) A possible effect of cyanogenic glucoside in sorghum on animal requirements for sulfur. The Journal of Agricultural Science 84, 377–379.
| A possible effect of cyanogenic glucoside in sorghum on animal requirements for sulfur.Crossref | GoogleScholarGoogle Scholar |