The Fractionation of the Nitrogen Compounds of Wheat Flour on Sephadex Collumns

Abstract

Bagdasarian et al. (1964) have discussed the difficulties in separating compounds of low molecular weight from proteins, and the advantages of using a volatile solvent, such as phenol~acetic acid~water (1 : 1 : 1, w/v/v) (referred to as solvent A in the remainder of this paper), to achieve this separation. Trichloroacetic acid is also efficient in effecting this separation (Bagdasarian et al. 1964), but it dissolves glycoproteins (Bell 1963; Stenzel et al. 1964 ; Jennings and Watt 1967) and some of the wheat flour proteins (Bell 1963) and is difficult to remove from solutions.