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Chromosome composition of an F2 Triticum aestivum × T. turgidum spp. durum cross analysed by DArT markers and MCFISH

Friederike S. Eberhard A , Peng Zhang B , Anke Lehmensiek A , Ray A. Hare C , Steven Simpfendorfer C and Mark W. Sutherland A D
+ Author Affiliations
- Author Affiliations

A Centre for Systems Biology, University of Southern Queensland, Toowoomba, Qld 4350, Australia.

B University of Sydney, Plant Breeding Institute, Cobbitty, NSW 2570, Australia.

C New South Wales Industry and Investment, Tamworth Agricultural Institute, Tamworth, NSW 2340, Australia.

D Corresponding author. Email:

Crop and Pasture Science 61(8) 619-624
Submitted: 16 April 2010  Accepted: 20 June 2010   Published: 13 August 2010


This study has employed multicolour fluorescence in situ hybridisation (MCFISH) and Diversity Arrays Technology (DArT) markers to determine the segregation of parental A, B and D genome material into the progeny of a cross between a hexaploid bread wheat (Triticum aestivum L. var. 2-49) and a tetraploid durum wheat [T. turgidum L. spp. durum (Desf.) var. Bellaroi]. In the F2 progeny from a 2-49/Bellaroi cross, 82 out of 83 F2 plants investigated with DArT analysis carried some D genome material, principally as entire chromosomes, while 40 plants included at least one complete copy of all seven D genome chromosomes. Twelve plants containing partial D chromosomes were identified. MCFISH analysis of 26 additional F2 plants of the same cross showed that all 26 plants contained varying amounts of D genome material of which three carried single A-D translocations. In addition two telocentric D genome chromosomes were detected. The D genome content of each line and the breakpoint positions of the three A-D translocations were confirmed with DArT marker analysis. Overall results indicate a random recombination of A and B genome loci from the hexaploid female parent and the tetraploid male parent in this F2 population and a significant retention of the maternal D genome material. This study illustrates that the combined application of the MCFISH and DArT techniques provides a powerful approach for the analysis of crosses between cereal genotypes of different ploidy.

Additional keywords: DArT, durum wheat, hexaploid × durum crosses, MCFISH.


The authors would like to thank Professor Grant Daggard (USQ) for his critical reading of the manuscript. This work was funded by the Grains Research and Development Corporation, Australia.


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