Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Comparing adaptive responses of new and old lucerne (Medicago sativa) genotypes under irrigated Mediterranean conditions

D. Baxevanos https://orcid.org/0000-0003-3771-8588 A * , O. Voulgari B , C. Pankou https://orcid.org/0000-0003-2144-4391 A , M. D. Yiakoulaki B and I. T. Tsialtas C
+ Author Affiliations
- Author Affiliations

A Hellenic Agricultural Organization ‘Demeter’, Institute of Industrial and Forage Crops, 41335 Larissa, Greece.

B Department of Range Science, Faculty of Agriculture Forestry and Natural Environment, School of Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124, Thessaloniki, Greece.

C Faculty of Agriculture, Laboratory of Agronomy, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

* Correspondence to: baxevano@gmail.com

Handling Editor: Mary-Jane Rogers

Crop & Pasture Science 73(6) 679-691 https://doi.org/10.1071/CP21234
Submitted: 19 December 2020  Accepted: 28 October 2021   Published: 11 February 2022

© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

Lucerne (Medicago sativa L.) is a major perennial forage legume worldwide. In Greece, new cultivars have been introduced mainly from the USA, Australia and Italy. However, some of these cultivars have not been tested locally before their widespread release. A field experiment was conducted from 2013 to 2016 in central Greece to compare the performance of 22 lucerne genotypes, including both local Greek and introduced genotypes, under irrigated Mediterranean conditions. There were three harvests in 2013 and five in each of the following years. Measurements included annual and total dry matter (DM) yield, harvest ratios, quality traits and agronomic parameters. Yields after the second year declined by 11.9–26.4%, possibly due to summer heat stress and reduced plant survival. However, several semi-winter-active cultivars were more persistent (by 19.4%) than highly winter-active cultivars. Three local, semi-winter-active cultivars and one introduced highly winter-active cultivar were the top performers for total DM yield (63.8–67.3 t DM/ha). Forage nutritive values showed small differences among cultivars. Evaluation of seasonal yield distribution was effective for comparing adaptive responses of genotypes in relation to the winter activity effect. Spring harvest ratio was more indicative (r = 0.92) of genotype total DM yield than was plant survival (r = 0.70). Specifically, highly winter-active cultivars showed higher autumn and spring harvests (by 36.4% and 7.9%, respectively) than semi-winter-active cultivars, which produced higher summer yields (by 35.7%). A regional program to breed more winter-active genotypes, which can capitalise on the longer preceding season with greater stand persistence, heat tolerance, summer productivity and height (>82 cm) and with more nodes (>18), could be beneficial for forage yield increase under the anticipated climatic changes.

Keywords: alfalfa, dry matter production, forage legumes, forage quality, genotype x environment interaction, GGE biplots, Medicago sativa, stand persistence.


References

Angus JF, Gault RR, Peoples MB, Stapper M, van Herwaarden AF (2001) Soil water extraction by dryland crops, annual pastures and lucerne in south-eastern Australia. Australian Journal of Agricultural Research 52, 183–192.
Soil water extraction by dryland crops, annual pastures and lucerne in south-eastern Australia.Crossref | GoogleScholarGoogle Scholar |

Annicchiarico P (2002) Genotype × environment interactions: challenges and opportunities for plant breeding and cultivar recommendations. Plant Production and Protection Paper 174. Food and Agriculture Organization of the United Nations, Rome, Italy.

Annicchiarico P (2006) Diversity, genetic structure, distinctness and agronomic value of Italian lucerne (Medicago sativa L.) landraces. Euphytica 148, 269–282.
Diversity, genetic structure, distinctness and agronomic value of Italian lucerne (Medicago sativa L.) landraces.Crossref | GoogleScholarGoogle Scholar |

Annicchiarico P, Scotti C, Carelli M, Pecetti L (2010) Questions and avenues for lucerne improvement. Czech Journal of Genetics and Plant Breeding 46, 1–13.
Questions and avenues for lucerne improvement.Crossref | GoogleScholarGoogle Scholar |

Annicchiarico P, Pecetti L, Torricelli R (2012) Impact of landrace germplasm, non-conventional habit and regional cultivar selection on forage and seed yield of organically grown lucerne in Italy. Journal of Agricultural Science 150, 345–355.
Impact of landrace germplasm, non-conventional habit and regional cultivar selection on forage and seed yield of organically grown lucerne in Italy.Crossref | GoogleScholarGoogle Scholar |

Annicchiarico P, Barrett B, Brummer EC, Julier B, Marshall AH (2015) Achievements and challenges in improving temperate perennial forage legumes. Critical Reviews in Plant Sciences 34, 327–380.
Achievements and challenges in improving temperate perennial forage legumes.Crossref | GoogleScholarGoogle Scholar |

AOAC (2000) ‘Official methods of analysis.’ 17th edn. (Association of Official Analytical Chemists: Gaithersburg, MD, USA)

Aranjuelo I, Irigoyen JJ, Pérez P, Sánchez-Díaz M (2007) Effect of elevated temperature and water availability on CO2 exchange and nitrogen fixation of nodulated alfalfa plants. Environmental and Experimental Botany 59, 99–108.
Effect of elevated temperature and water availability on CO2 exchange and nitrogen fixation of nodulated alfalfa plants.Crossref | GoogleScholarGoogle Scholar |

Baxevanos D, Goulas C, Tzortzios S, Mavromatis A (2008) Interrelationship among and repeatability of seven stability indices estimated from commercial cotton (Gossypium hirsutum L.) variety evaluation trials in three Mediterranean countries. Euphytica 161, 371–382.
Interrelationship among and repeatability of seven stability indices estimated from commercial cotton (Gossypium hirsutum L.) variety evaluation trials in three Mediterranean countries.Crossref | GoogleScholarGoogle Scholar |

Beuselinck PR, Bouton JH, Lamp WO, Matches AG, McCaslin MH, Nelson CJ, Rhodes LH, Sheaffer CC, Volenec JJ (1994) Improving legume persistence in forage crop systems. Journal of Production Agriculture 7, 311–322.
Improving legume persistence in forage crop systems.Crossref | GoogleScholarGoogle Scholar |

Biazzi E, Nazzicari N, Pecetti L, Brummer EC, Palmonari A, Tava A, Annicchiarico P (2017) Genome wide association mapping and genomic selection for alfalfa (Medicago sativa) forage quality traits. PLOS ONE 12, e0169234
Genome wide association mapping and genomic selection for alfalfa (Medicago sativa) forage quality traits.Crossref | GoogleScholarGoogle Scholar | 28068350PubMed |

Bingham ET, Groose RW, Woodfield DR, Kidwell KK (1994) Complementary gene interactions in alfalfa are greater in autotetraploids than diploids. Crop Science 34, 823–829.
Complementary gene interactions in alfalfa are greater in autotetraploids than diploids.Crossref | GoogleScholarGoogle Scholar |

Bolger TP, Matches AG (1990) Water-use efficiency and yield of sainfoin and alfalfa. Crop Science 30, 143–148.
Water-use efficiency and yield of sainfoin and alfalfa.Crossref | GoogleScholarGoogle Scholar |

Boller B, Posselt UK, Veronesi F (2010) ‘Fodder crops and amenity grasses.’ (Springer: New York)

Bolton JL (1962) ‘Alfalfa: botany, cultivation, and utilization.’ (Interscience Publishers: New York)

Bouton JH (2012) Breeding lucerne for persistence. Crop & Pasture Science 63, 95–106.
Breeding lucerne for persistence.Crossref | GoogleScholarGoogle Scholar |

Brough RC, Robison LR, Jackson RH (1977) The historical diffusion of alfalfa. Journal of Agronomic Education 6, 13–19.
The historical diffusion of alfalfa.Crossref | GoogleScholarGoogle Scholar |

Brummer EC (1999) Capturing heterosis in forage crop cultivar development. Crop Science 39, 943–954.
Capturing heterosis in forage crop cultivar development.Crossref | GoogleScholarGoogle Scholar |

Brummer EC, Shah MM, Luth D (2000) Reexamining the relationship between fall dormancy and winter hardiness in alfalfa. Crop Science 40, 971–977.
Reexamining the relationship between fall dormancy and winter hardiness in alfalfa.Crossref | GoogleScholarGoogle Scholar |

Byerly RL (1970) The use of multiple regression and path analysis in analyzing success in journalism at Iowa State University. PhD Thesis, Iowa State University, Ames, IA, USA.

Cunningham SM, Volenec JJ (1998) Seasonal carbohydrate and nitrogen metabolism in roots of contrasting alfalfa (Medicago sativa L.) cultivars. Journal Plant Physiology 153, 220–225.
Seasonal carbohydrate and nitrogen metabolism in roots of contrasting alfalfa (Medicago sativa L.) cultivars.Crossref | GoogleScholarGoogle Scholar |

Cunningham SM, Volenec JJ, Teuber LR (1998) Plant survival and root and bud composition of alfalfa population selected for contrasting fall dormancy. Crop Science 38, 962–969.
Plant survival and root and bud composition of alfalfa population selected for contrasting fall dormancy.Crossref | GoogleScholarGoogle Scholar |

Enriquez-Hidalgo D, Cruz T, Teixeira DL, Steinfort U (2020) Phenological stages of Mediterranean forage legumes, based on the BBCH scale. Annals of Applied Biology 176, 357–368.
Phenological stages of Mediterranean forage legumes, based on the BBCH scale.Crossref | GoogleScholarGoogle Scholar |

Frutos E, Galindo MP, Leiva V (2014) An interactive biplot implementation in R for modeling genotype-by-environment interaction. Stochastic Environmental Research and Risk Assessment 28, 1629–1641.
An interactive biplot implementation in R for modeling genotype-by-environment interaction.Crossref | GoogleScholarGoogle Scholar |

Giannakopoulos C, Kostopoulou E, Varotsos KV, Tziotziou K, Plitharas A (2011) An integrated assessment of climate change impacts for Greece in the near future. Regional Environmental Change 11, 829–843.
An integrated assessment of climate change impacts for Greece in the near future.Crossref | GoogleScholarGoogle Scholar |

Hellenic Statistical Authority (2019) Fodder plants for hey, areas and production, by Region and Regional Unities. Hellenic Statistical Authority, Athens, Greece. Available at https://www.statistics.gr/en/statistics/-/publication/SPG06/-/

Hoy MD, Moore KJ, George JR, Brummer EC (2002) Alfalfa yield and quality as influenced by establishment method. Agronomy Journal 94, 65–71.
Alfalfa yield and quality as influenced by establishment method.Crossref | GoogleScholarGoogle Scholar |

Jeranyama P, Garcia AD (2004) Understanding relative feed value (RFV) and relative forage quality (RFQ). Extension Extra, Paper 352. South Dakota State University, Brookings, SD, USA.

Karlen DL, Lemunyon JL, Singer JW (2007) Forages for conservation and improved soil quality. In ‘Forages. Volume II. The science of grassland agriculture’. (Eds RF Barnes, CJ Nelson, KJ Moore, M Collins) pp. 149–166. (Blackwell Publishing Professional: Ames, IA, USA)

Katić S, Mihailović V, Milić D, Karagić Đ, Mikić A (2004) Divergence of alfalfa cultivars depending on geographic origin. Natura Montenegrina 3, 197–202.

Kontsiotou EK (2005) ‘Alfalfa cultivation and use.’ (Publications Agrotypos SA: Athens, Greece) [in Greek]

Lamb JFS, Sheaffer CC, Rhodes LH, Sulc RM, Undersander DJ, Brummer EC (2006) Five decades of alfalfa cultivar improvement: impact on forage yield, persistence, and nutritive value. Crop Science 46, 902–909.
Five decades of alfalfa cultivar improvement: impact on forage yield, persistence, and nutritive value.Crossref | GoogleScholarGoogle Scholar |

Li Y, Su D (2017) Alfalfa water use and yield under different sprinkler irrigation regimes in north arid regions of China. Sustainability 9, 1380
Alfalfa water use and yield under different sprinkler irrigation regimes in north arid regions of China.Crossref | GoogleScholarGoogle Scholar |

Lloveras J, Ferran J, Alvarez A, Torres L (1998) Harvest management effects on alfalfa production and quality in Mediterranean areas. Grass and Forage Science 53, 88–92.
Harvest management effects on alfalfa production and quality in Mediterranean areas.Crossref | GoogleScholarGoogle Scholar |

Luo XY, Li H, Wang FG (2001) Analysis of correlation and path on alfalfa yield with different environments and years. In ‘Prospects of Grassland Science and Industry for the 21st Century. Paper collection of international conference on grassland science and industry’. pp. 351–354. (Chinese Grassland Society/China Association of Agricultural Societies: Beijing, China)

Moore JE (1970) Procedure for the two-stage in vitro digestion of forages. In ‘Nutrition research techniques for domestic and wild animals’. (Ed. LE Harris) pp. 5001–5005. (Utah State University: Logan, UT, USA)

Nie Z, Norton MR (2009) Stress tolerance and persistence of perennial grasses: the role of the summer dormancy trait in temperate Australia. Crop Science 49, 2405–2411.
Stress tolerance and persistence of perennial grasses: the role of the summer dormancy trait in temperate Australia.Crossref | GoogleScholarGoogle Scholar |

Oliveira JA, Lopez JE, Palencia P (2013) Agromorphological characterization, cyanogenesis and productivity of accessions of white clover (Trifolium repens L.) collected in northern Spain. Czech Journal of Genetics and Plant Breeding 49, 24–35.
Agromorphological characterization, cyanogenesis and productivity of accessions of white clover (Trifolium repens L.) collected in northern Spain.Crossref | GoogleScholarGoogle Scholar |

Pecetti L, Annicchiarico P, Scotti C, Paolini M, Nanni V, Palmonari A (2017) Effects of plant architecture and drought stress level on lucerne forage quality. Grass and Forage Science 72, 714–722.
Effects of plant architecture and drought stress level on lucerne forage quality.Crossref | GoogleScholarGoogle Scholar |

Peel MC, Finlayson BL, McMahon TA (2007) Updated world map of the Köppen-Geiger climate classification. Hydrology and Earth Systems. Sciences 11, 1633–1644.
Updated world map of the Köppen-Geiger climate classification. Hydrology and Earth Systems.Crossref | GoogleScholarGoogle Scholar |

Pembleton KG, Cunningham SM, Volenec JJ (2010a) Effect of summer irrigation on seasonal changes in taproot reserves and the expression of winter dormancy/activity in four contrasting lucerne cultivars. Crop & Pasture Science 61, 873–884.
Effect of summer irrigation on seasonal changes in taproot reserves and the expression of winter dormancy/activity in four contrasting lucerne cultivars.Crossref | GoogleScholarGoogle Scholar |

Pembleton KG, Smith RS, Rawnsley RP, Donaghy DJ, Humphries AW (2010b) Genotype by environment interactions of lucerne (Medicago sativa L.) in a cool temperate climate. Crop & Pasture Science 61, 493–502.
Genotype by environment interactions of lucerne (Medicago sativa L.) in a cool temperate climate.Crossref | GoogleScholarGoogle Scholar |

Picasso VD, Casler MD, Undersander D (2019) Resilience, stability, and productivity of alfalfa cultivars in rainfed regions of north America. Crop Science 59, 800–810.
Resilience, stability, and productivity of alfalfa cultivars in rainfed regions of north America.Crossref | GoogleScholarGoogle Scholar |

Radović J, Sokolović D, Marković J (2009) Alfalfa-most important perennial forage legume in animal husbandry. Biotechnology in Animal Husbandry 25, 465–475.
Alfalfa-most important perennial forage legume in animal husbandry.Crossref | GoogleScholarGoogle Scholar |

Rotili P, Gnocchi G, Scotti C (1998) Lucerne breeding for dehydration: allometric growth and quality. In ‘Proceedings of 21st EUCARPIA fodder crops and amenity grasses meeting, breeding for a multifunctional agriculture’. (Eds B Boller, FJ Stadelmann) pp. 20–23. (FAL: Zürich, Switzerland)

Russelle MP, Lamb JFS, Montgomery BR, Elsenheimer DW, Miller BS, Vance CP (2001) Alfalfa rapidly remediates excess inorganic nitrogen at a fertilizer spill site. Journal of Environmental Quality 30, 30–36.
Alfalfa rapidly remediates excess inorganic nitrogen at a fertilizer spill site.Crossref | GoogleScholarGoogle Scholar | 11215664PubMed |

Schnurr JA, Jung H-JG, Samac DA (2007) A comparative study of alfalfa and Medicago truncatula stem traits: morphology, chemical composition, and ruminal digestibility. Crop Science 47, 1672–1680.
A comparative study of alfalfa and Medicago truncatula stem traits: morphology, chemical composition, and ruminal digestibility.Crossref | GoogleScholarGoogle Scholar |

Schwab PM, Barnes DK, Sheaffer CC (1996) The relationship between field winter injury and fall growth score for 251 alfalfa cultivars. Crop Science 36, 418–426.
The relationship between field winter injury and fall growth score for 251 alfalfa cultivars.Crossref | GoogleScholarGoogle Scholar |

Smith D (1961) Association of fall growth habit and winter survival in alfalfa. Canadian Journal of Plant Science 41, 224–231.
Association of fall growth habit and winter survival in alfalfa.Crossref | GoogleScholarGoogle Scholar |

Teuber LR, Taggard KL, Gibbs LK, McCaslin MA, Peterson MA, Barnes DK (1998) Fall dormancy: standard tests to characterize alfalfa cultivars. Standard Tests. In ‘North American Alfalfa Improvement Conference’. St. Paul, MN, USA. Available at http://www.naaic.org/stdtests/Dormancy2.html [Accessed 12 September 2019]

Tilley JMA, Terry RA (1963) A two-stage technique for in vitro digestion of forage crops. Grass and Forage Science 18, 104–111.
A two-stage technique for in vitro digestion of forage crops.Crossref | GoogleScholarGoogle Scholar |

Undersander D (2003) The new Relative Forage Quality Index – concept and use. University of Wisconsin Extension, Madison, WI, USA.

Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar | 1660498PubMed |

Ventroni LM, Volenec JJ, Cangiano CA (2010) Fall dormancy and cutting frequency impact on alfalfa yield and yield components. Field Crops Research 119, 252–259.
Fall dormancy and cutting frequency impact on alfalfa yield and yield components.Crossref | GoogleScholarGoogle Scholar |

Veronesi F, Huyghe G, Delgado I (2006) Lucerne breeding in Europe: results and research strategies for future developments. PASTOS 36, 143–158.

Vlachostergios D, Baxevanos D (2015) ‘Greek legume and forage cultivars.’ pp. 4–7. (Institute of Industrial and Fodder Crops: Larissa, Greece) [in Greek]

Vogel KP, Lamb JFS (2007) Forage breeding. In ‘Forages. Volume II. The science of grassland agriculture’. (Eds RF Barnes, CJ Nelson, KJ Moore, M Collins) pp. 427–438. (Blackwell Publishing Professional: Ames, IA, USA)

Volenec JJ, Cherney JH, Johnson KD (1987) Yield components, plant morphology, and forage quality of alfalfa as influenced by plant population. Crop Science 27, 321–326.
Yield components, plant morphology, and forage quality of alfalfa as influenced by plant population.Crossref | GoogleScholarGoogle Scholar |

Volenec JJ, Cunningham SM, Haagenson DM, Berg WK, Joern BC, Wiersma DW (2002) Physiological genetics of alfalfa improvement: past failures, future prospects. Field Crops Research 75, 97–110.
Physiological genetics of alfalfa improvement: past failures, future prospects.Crossref | GoogleScholarGoogle Scholar |

Wassie M, Zhang W, Zhang Q, Ji K, Chen L (2019) Effect of heat stress on growth and physiological traits of alfalfa (Medicago sativa L.) and a comprehensive evaluation for heat tolerance. Agronomy 9, 597
Effect of heat stress on growth and physiological traits of alfalfa (Medicago sativa L.) and a comprehensive evaluation for heat tolerance.Crossref | GoogleScholarGoogle Scholar |

Yan W (2002) Singular-value partitioning in biplot analysis of multi-environment trial data. Agronomy Journal 94, 990–996.
Singular-value partitioning in biplot analysis of multi-environment trial data.Crossref | GoogleScholarGoogle Scholar |

Zang WM, Wang CZ, Yang YX (2010) Production performance of different lucerne varieties in China. New Zealand Journal of Agricultural Research 48, 481–488.
Production performance of different lucerne varieties in China.Crossref | GoogleScholarGoogle Scholar |

Zhang T-J, Kang J-M, Guo W-S, Zhao Z-X, Xu Y-P, Yan X-D, Yang Q-C (2014) Yield evaluation of twenty-eight alfalfa cultivars in Hebei province of China. Journal of Integrative Agriculture 13, 2260–2267.
Yield evaluation of twenty-eight alfalfa cultivars in Hebei province of China.Crossref | GoogleScholarGoogle Scholar |