Australasian Plant Disease Notes Australasian Plant Disease Notes Society

Molecular evidence for association of Tomato leaf curl New Delhi virus with leaf curl disease of papaya (Carica papaya L.) in India

S. K. Raj A C , S. K. Snehi A , M. S. Khan A , R. Singh A and A. A. Khan B
+ Author Affiliations
- Author Affiliations

A Molecular Virology Laboratory, National Botanical Research Institute, Lucknow, UP 226001, India.

B Plant Virology Laboratory, Aligarh Muslim University, Aligarh, UP 202002, India.

C Corresponding author. Email:

Australasian Plant Disease Notes 3(1) 152-155
Submitted: 18 May 2008  Accepted: 10 November 2008   Published: 20 November 2008


Association of Tomato leaf curl New Delhi virus with leaf curl disease of papaya (Carica papaya L.) was detected by polymerase chain reaction using begomovirus-specific primers and confirmed by highest sequence similarities and close phylogenetic relationships.


The authors express their gratitude to the Director, National Botanical Research Institute, Lucknow for facilities and Department of Science and Technology, New Delhi for funding to AA Khan.


Chang LS, Lee YS, Su HJ, Hung TH (2003) First report of Papaya leaf curl virus Infecting papaya plants in Taiwan. Plant Disease 87, 204.
CrossRef | open url image1

Dellaporta SJ, Wood J, Hick JB (1983) A plant DNA minipreparation: Version II. Plant Molecular Biology Reporter 1, 19–21.
CrossRef | CAS | open url image1

Fauquet CM, Briddon RW, Brown JK, Moriones E, Stanley J, Zerbini M, Zhou X (2008) Geminivirus strain demarcation and nomenclature. Archives of Virology 153, 783–821.
CrossRef | CAS | PubMed | open url image1

Harrison BD (1985) Advances in Geminivirus research. Annual Review of Phytopathology 23, 55–82.
CrossRef | CAS | open url image1

Huang JF, Zhou XP (2006) First report of Papaya leaf curl China virus infecting Corchoropsis timentosa in China. Plant Pathology 55, 291.
CrossRef | open url image1

Morgenstern B (1998) DiAlign2 Improvement of the segment to the segment approach to multiple sequence alignment. Bioinformatics (Oxford, England) 15, 203–210. open url image1

Nadeem A, Mehmood T, Tahir M, Khalid S, Xing Z (1997) First report of papaya leaf curl disease in Pakistan. Plant Disease 81, 1333.
CrossRef | open url image1

Nariani TK (1956) Leaf curl of papaya. Indian Phytopathology 9, 151–157. open url image1

Saxena S, Hallan V, Singh BP, Sane PV (1998a) Leaf curl disease of Carica papaya from India may be caused by bipartite geminivirus. Plant Disease 82, 126.
CrossRef | open url image1

Saxena S, Hallan V, Singh BP, Sane PV (1998b) Evidence from nucleic acid hybridization test for a geminivirus infection causing leaf curl disease of papaya in India. Indian Journal of Experimental Biology 36, 229–232.
open url image1

Singh R (2005) Molecular characterization of a virus causing yellow mosaic disease in Cucurbita maxima and development of diagnostics for detection of the virus. PhD Thesis, University of Lucknow, Lucknow.

Singh A (2006) Studies of occurrence of papaya viruses in eastern Uttar Pradesh and their possible management approaches. PhD Thesis, University of Gorakhpur.

Singh U , Wadhwani AM , Johri BM (1983) Carica papaya. In ‘Dictionary of economic plants in India’. (Ed. PL Jaiswal) p. 76. (Indian Council of Agriculture Research: New Delhi)

Thomas KM, Krishanaswamy CS (1939) Leaf crinkle – A transmissible disease of Papaya. Current Science 8, 316–317. open url image1

Wang X, Xie Y, Zhou X (2004) Molecular characterization of two distinct Begomoviruses from papaya in China. Virus Genes 29, 303–309.
CrossRef | CAS | PubMed | open url image1

Wyatt SD, Brown JK (1996) Detection of subgroup III geminivirus isolates in leaf extracts by degenerate primers and polymerase chain reaction. Phytopathology 86, 1288–1293.
CrossRef | open url image1

Zhang LB, Zhou GH, Li HP, Zhang SG (2005) Molecular characterization of Papaya leaf curl virus infecting Carica papaya in Guangzhou and its biological test. Scientia Agricultura Sinica 38, 1805–1810.
open url image1

Full Text PDF (393 KB) Export Citation