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Article     |     Next >>   Contents Vol 61(6)

Isolation and characterisation of microsatellite loci in the bush stone-curlew (Burhinus grallarius), a declining Australian bird

Robert A. B. Mason A D E, Catherine Price B, Walter E. Boles A, Karen-Anne Gray A, Edwina Rickard A C, Mark D. B. Eldridge A and Rebecca N. Johnson A

A Australian Museum Research Institute, Australian Centre for Wildlife Genomics, Australian Museum, 6 College Street, Sydney, NSW 2010, Australia.
B School of Biological Sciences, The University of Sydney, Sydney, NSW 2006, Australia.
C Department of Clinical Genetics, The Children’s Hospital at Westmead, Westmead, NSW 2145, Australia.
D Present address: School of Biological Sciences, The University of Queensland, St Lucia, Qld 4072, Australia, and Hawai‘i Institute of Marine Biology, University of Hawai‘i at Mānoa, 46-007 Lilipuna Road, Kane‘ohe, HI 96744, USA.
E Corresponding author. Email: robert.mason1@uqconnect.edu.au

Australian Journal of Zoology 61(6) 421-423 http://dx.doi.org/10.1071/ZO13059
Submitted: 25 July 2013  Accepted: 6 January 2014   Published: 28 January 2014

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The bush stone-curlew (Burhinus grallarius Latham), a ground-nesting nocturnal bird, is endangered in southern Australia due to habitat modification and introduced predators. To provide tools for conservation, ecological and behavioural studies, we isolated variable microsatellite repeat sequences and designed primers for PCR amplification in this species. Primer pairs were developed and levels of diversity were assessed for eight microsatellite loci, including one locus linked to the gene encoding Microtubule-Associated Protein 2, a protein important for behavioural imprinting in birds, and one sex-linked locus. Isolated loci contained allelic diversity of between 5 and 17 alleles.

Additional keywords: Burhinidae, conservation genetics, hemizygous, hybridisation capture and enrichment, microsatellite, Microtubule-Associated Protein 2 (MAP2), non-isotopic screening.


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