Roles of poly (ADP-ribose) polymerase 1 activation and cleavage in induction of multi-oocyte ovarian follicles in the mouse by 3-nitropropionic acid
Quanwei Wei A * , Guoyun Wu A * , Jun Xing A B , Dagan Mao A , Reinhold J. Hutz C and Fangxiong Shi
A D
+ Author Affiliations
- Author Affiliations
A Laboratory of Animal Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
B Department of Animal Husbandry and Veterinary Medicine, Jiangsu Polytechnic College of Agriculture and Forestry, Jurong 212400, China.
C Department of Biological Sciences, University of Wisconsin-Milwaukee, Lapham Hall, Milwaukee, WI 53211-0413, USA.
D Corresponding author. Email: fxshi@njau.edu.cn
Reproduction, Fertility and Development 31(5) 1017-1032 https://doi.org/10.1071/RD18406
Submitted: 8 October 2018 Accepted: 7 January 2019 Published: 6 March 2019
Abstract
3-nitropropionic acid (3-NPA) is known to be a mitochondrial toxin produced by plants and fungi, which may produce DNA damage in cells. However, studies of its reproductive toxicology are lacking. We know that poly(ADP-ribose) polymerase (PARP) plays an important role in a large variety of physiological processes and is involved in DNA repair pathways. The present study was therefore aimed at exploring the involvement of PARP-1 activation and cleavage after 3-NPA stimulation in female mice. We observed an increased number of atretic follicles and multi-oocyte follicles (MOFs) after treatment with 3-NPA and serum concentrations of 17β-oestradiol and progesterone were significantly reduced. Our results provide evidence that PARP-1 cleavage and activational signals are involved in pathological ovarian processes stimulated by 3-NPA. In addition, total superoxide dismutase, glutathione peroxidase and catalase activities were significantly increased, whereas succinate dehydrogenase was decreased in a dose-dependent manner. Results from our in vitro study similarly indicated that 3-NPA inhibited the proliferation of mouse granulosa cells and increased apoptosis in a dose-dependent manner. In summary, 3-NPA induces granulosa cell apoptosis, follicle atresia and MOFs in the ovaries of female mice and causes oxidative stress so as to disrupt endogenous hormonal systems, possibly acting through PARP-1 signalling.
Additional keywords: 3-nitropropionic acid, 3-NPA, follicular granulosa cell, MOFs.
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