RD25064Platelet-rich plasma strategy against freezing damage in ram spermatozoa: its effect on miRNA, ion channels, growth factors, lipids and oxidative stress


From a molecular point of view, 5% platelet-rich plasma (PRP) can be added to ram semen diluents. It is not recommended to add PRP at 10% concentration to the ram semen diluent. PRP at 5% increases sperm motility, vitality and antioxidant activity after thawing. PRP at 5 % improves frozen-thawed sperm quality, enhancing its cryoprotective role. PRP supplementation alters gene and protein expression in frozen-thawed sperm. Image by İbrahim Halil Güngör.