Effect of arachidonic acid on pre- and post-hatching in vitro bovine embryo development
L. González-Brusi A , A. Pérez-Gómez A , A. C. Quiroga A , C. Tamargo B , P. Ramos-Ibeas A and P. Bermejo-Álvarez
A *
+ Author Affiliations
- Author Affiliations
A Animal Reproduction Department, INIA, CSIC, Madrid 28040, Spain.
B Department of Animal Selection and Reproduction, SERIDA, Gijón 33394, Spain.
Reproduction, Fertility and Development 35(12) 614-621 https://doi.org/10.1071/RD23053
Published online: 11 July 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)
Abstract
Context: Arachidonic acid (AA) is the precursor of prostaglandins, which may play autocrine roles during early embryo development.
Aims: To test the developmental effects of addition of AA to pre- and post-hatching culture media on in vitro–produced bovine embryos.
Methods: Pre-hatching effects of AA were tested by culturing bovine zygotes in synthetic oviductal fluid (SOF) supplemented with 100 or 333 μM AA. Post-hatching effects of AA were tested by culturing Day 7 blastocysts in N2B27 supplemented with 5, 10, 20 or 100 μM AA up to Day 12.
Key results: Pre-hatching development to blastocyst was completely abrogated at 333 μM AA, whereas blastocyst rates and cell numbers were not altered at 100 μM AA. Impaired post-hatching development was observed at 100 μM AA, whereas no effect on survival rates was noted at 5, 10 and 20 μM AA. However, a significant reduction in Day 12 embryo size was observed at 10 and 20 μM AA. Hypoblast migration, epiblast survival and formation of embryonic-disc-like structures were unaffected at 5–10 μM AA. AA exposure downregulated the genes PTGIS, PPARG, LDHA and SCD in Day 12 embryos.
Conclusions: Pre-hatching embryos are mostly irresponsive to AA, whereas AA was observed to have negative effects during early post-hatching development.
Implications: AA does not improve in vitro bovine embryo development and is not required up to early post-hatching stages.
Keywords: arachidonic, bovine, conceptus, elongation, embryo development, lipids, PPARG, prostaglandin, SCD, trophectoderm.
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