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Plant function and evolutionary biology
RESEARCH ARTICLE

The seed-borne Southern bean mosaic virus hinders the early events of nodulation and growth in Rhizobium-inoculated Phaseolus vulgaris L.

Mariadaniela López A , Nacira Muñoz B C , Hernan Ramiro Lascano B C and María Luisa Izaguirre-Mayoral D E
+ Author Affiliations
- Author Affiliations

A Universidad Centroccidental Lisandro Alvarado, Postgrado de Agronomia, Laboratorio de Virologia, Barquisimeto 03023, Venezuela.

B Instituto de Fisiología y Recursos Genéticos Vegetales, Centro de Investigaciones Agropecuarias – Instituto Nacional de Tecnología Agropecuaria, 5119 Córdoba, Argentina.

C Cátedra de Fisiología Vegetal, Facultad de Ciencias Exactas Físicas y Naturales, Universidad Nacional de Córdoba, Avenida Vélez Sársfield 1611 Córdoba, Argentina.

D Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, apartado postal 21827 Caracas 1020-A, Venezuela.

E Corresponding author. Email: mlizaguirre@gmail.com

Functional Plant Biology 44(2) 208-218 https://doi.org/10.1071/FP16180
Submitted: 12 May 2016  Accepted: 6 September 2016   Published: 20 October 2016

Abstract

To simulate seed-borne virus transmission, a noninvasive protocol was designed to infect the radicle of germinating seeds, with 100% effectiveness. Preinfection of 24-h-old black bean (Phaseolus vulgaris L.) radicles by Southern bean mosaic virus (SBMV) followed by Rhizobium inoculation 48 h later caused a drastic reduction in root nodulation. Results were attributed to active virus replication within the elongating zone of the radicle at least 32 h before Rhizobium inoculation, which elicited severe anatomical malformations; an abnormal accumulation of apoplastic reactive oxygen species in the rhizodermis, cortex, inner cortical and endodermic root cells; the formation of atypical root hair tips and the collapse of 94% of the root hairs in the SBMV-preinfected radicles. Adult SBMV-preinfected plants showed exacerbated virus symptoms and 80% growth reduction ascribed to major virus-induced ultrastructural alterations in the nodules. The accumulation of ureides, α−amino acids and total reducing sugars in the leaves and nodules of SBMV-preinfected plants are indicators of the hindering effects of SBMV infection on N2 fixation and ureide catabolism, causing N starvation. The exogenous addition of 1 or 4 μM naringenin, genistein or daidzein did not counteract the deleterious effects of SBMV preinfection on nodulation.

Additional keywords: amino acids, nodule ultrastructure, reactive oxygen species, root hairs, total reducing sugars, ureides.


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