Inhibitors of lysine biosynthesis enzymes as potential new herbicides

Emily R. R. Mackie; Mirrin V. McKay; Andrew S. Barrow; Tatiana P. Soares da Costa

doi:10.1071/FP25030

REVIEW (Open Access)

Previous Next Contents Vol 52(5)

Inhibitors of lysine biosynthesis enzymes as potential new herbicides

Emily R. R. Mackie ^A ^# , Mirrin V. McKay ^A ^# , Andrew S. Barrow ^A and Tatiana P. Soares da Costa

^A ^*

+ Author Affiliations

- Author Affiliations

^A School of Agriculture, Food and Wine and Waite Research Institute, The University of Adelaide, Waite Campus, Urrbrae, SA 5064, Australia.

^* Correspondence to: tatiana.soaresdacosta@adelaide.edu.au

^# These authors contributed equally to this paper

Handling Editor: Rana Munns

Functional Plant Biology 52, FP25030 https://doi.org/10.1071/FP25030

Submitted: 30 January 2025 Accepted: 28 April 2025 Published: 12 May 2025

© 2025 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)

Abstract

Lysine is an amino acid that is essential for the growth and development of all organisms owing to its role in a plethora of critical biological functions and reactions. In plants, lysine is synthesised via five sequential enzyme-catalysed reactions collectively known as the diaminopimelate (DAP) pathway, whereas animals are reliant on their plant dietary intake to obtain lysine. Given that lysine is one of the most nutritionally limiting amino acids, several studies have focused on developing strategies to modulate the activity of DAP pathway enzymes to improve the nutritional value of crops. More recently, research has emerged on the potential of inhibiting DAP pathway enzymes for the development of herbicides with a novel mode of action. Over reliance on a small number of modes of action has led to a herbicide resistance crisis, necessitating the development of new modes of action to which no resistance exists. As such, the first herbicidal inhibitors of the DAP pathway have been developed, which target the first three enzymes in lysine biosynthesis. This review explores the structure, function, and inhibition of these enzymes, as well as highlighting promising avenues for the future development of new plant lysine biosynthesis inhibitors.

Keywords: amino acid, catalysis, enzymes, herbicide development, herbicide resistance, inhibitors, lysine, weeds.

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