Distribution pattern of cytoplasmic organelles, spindle integrity, oxidative stress, octamer-binding transcription factor 4 (Oct4) expression and developmental potential of oocytes following multiple superovulation
Guruprasad Kalthur A E , Sujith Raj Salian A , Ramya Nair A , Jemey Mathew A , Satish Kumar Adiga A , Sneha Guruprasad Kalthur B , Dimphy Zeegers C and M. Prakash Hande C D
+ Author Affiliations
- Author Affiliations
A Division of Clinical Embryology, Department of Obstetrics and Gynecology, Kasturba Medical College, Manipal University, Manipal, 576104, India.
B Department of Anatomy, Kasturba Medical College, Manipal University, Manipal, 576104, India.
C Genome Stability Laboratory, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, 117597, Singapore.
D Tembusu College, National University of Singapore, 138598, Singapore.
E Corresponding author. Email: guru.kalthur@manipal.edu
Reproduction, Fertility and Development 28(12) 2027-2038 https://doi.org/10.1071/RD15184
Submitted: 9 May 2015 Accepted: 9 June 2015 Published: 15 July 2015
Abstract
The aim of the present study was to determine the effects of repeated superovulation on oocyte quality and embryo developmental potential. Female Swiss albino mice were injected with 5 IU pregnant mare’s serum gonadotropin followed 48 h by 10 IU human chorionic gonadotropin. Mice were superovulated up to four times with a gap of 7 days between each superovulation cycle. Ovarian weight increased significantly with an increasing number of superovulation cycles. Although the first stimulation cycle resulted in a threefold increase in the number of oocytes, the number of oocytes decreased gradually after subsequent stimulations. Increased cytoplasmic fragmentation, abnormal mitochondrial distribution, aggregation of Golgi apparatus, spindle damage, increased intracellular oxidative stress and a decrease in expression of octamer-binding transcription factor 4 (Oct4) expression were observed in these oocytes. Further, embryos derived from mice subjected to multiple stimulation cycles exhibited a low blastocyst rate, decreased hatching rate and increased apoptosis in blastocysts. In conclusion, the present study demonstrates that repeated superovulation adversely affects mouse oocyte quality by altering the distribution of cytoplasmic organelles, increasing oxidative stress and decreasing Oct4 expression, resulting in poor developmental potential of the embryos.
Additional keywords: embryo development, endoplasmic reticulum, Golgi apparatus, mitochondria, reactive oxygen species, spindle damage.
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